| Project/Area Number |
20590389
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
ISHIKAWA Naoshi Tokyo Metropolitan Institute of Gerontology, 研究員 (30184485)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA KenーIchi 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 研究員 (60159069)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKUBO Kaiyo 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 研究部長 (00154956)
NAKAMURA Ken-Ichi 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 研究員 (60159069)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 老化 / がん化 / ウェルナー症候群 / テロメア / FISH / 染色体組換え |
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| Research Abstract |
we measured terminal restriction fragment (TRF) lengths of 10 skin samples collected from extremities of 8 WS patients aged between 30 and 61 years that had been surgically amputated because of skin ulceration. Whereas the values of TRF length in younger WS patients (in their thirties) were within the normal range, those in older WS patients were markedly shorter relative to non-WS controls. Regression analyses indicated that the TRF length in WS was significantly shorter than that in controls (p <0.001). Furthermore, we found that TRF lengths in muscle adjacent to the examined epidermis were also significantly shorter than those of controls (p = 0.047). These data demonstrate for the first time that in vivo telomere loss is accelerated in systemic organs of WS patients, suggesting that abnormal telomere erosion is one of the major causes of early onset of age-related symptoms and a predisposition to sarcoma and carcinoma in WS.
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