Establishment of aspiration biopsy-nucleic acid diagnosis detecting IgH monoclonality in malignant lymphoma

• Project/Area Number: 20590570
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Osaka University
• Principal Investigator: TAKANO Toru Osaka University, 医学系研究科, 講師 (00263236)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 悪性遺リンパ腫 / 甲状腺 / IgH遺伝子 / モノクロナリティー / 穿刺吸引細胞診 / 悪性リンパ腫

Research Abstract

VR was performed in patients suspected having thyroid malignant lymphoma. 0 of 2 and 7 of 11 patients of Hashimoto's and malignant lymphoma, respectively, showed a positive result in monoclonality in the IgH gene. A single tube VR method, which requires only a microcentrifuge machine and thermal cycler, was established.

Research Products

• [Journal Article] The fifteen percent issue in molecular-based diagnosis of follicular thyroid carcinoma. (2011)
  • Author(s): Takano. T
  • Journal Title: Pathol Int 61 Pages: 165-166
  • Peer Reviewed
• [Journal Article] Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines. (2011)
  • Author(s): Yamada, H., Takano, T., Matsuzuka, F., Watanabe, M., Miyauchi, A., Iwatani, Y.
  • Journal Title: Genetics Mol Biol 34 Pages: 6-10
  • Peer Reviewed
• [Journal Article] Transcriptional activity of the 5'-flanking region of the thyroid transcription factor-1 gene in human thyroid cell lines (2011)
  • Author(s): Yamada, H, Takano T, et al.
  • Journal Title: Genetics Mol Biol Volume: 34 Pages: 6-10
  • Peer Reviewed
• [Journal Article] The fifteen percent issue in molecular-based diagnosis of follicular thyroid carcinoma (2011)
  • Author(s): Takano, T
  • Journal Title: Pathol Int Volume: 61 Pages: 165-166
  • Peer Reviewed
• [Journal Article] Messenger RNA quantification after fluorescence activated cell sorting using intracellular antigens. (2010)
  • Author(s): Yamada, H., Maruo, R., Watanabe, M., Hidaka, Y., Iwatani, Y., Takano, T.
  • Journal Title: Biochem Biophys Res Commun 397 Pages: 425-428
  • Peer Reviewed
• [Journal Article] mRNA quantification after FACS using in situ hybridization. (2010)
  • Author(s): Yamada, H., Maruo, R., Watanabe, M., Hidaka, Y., Iwatani, Y., Takano, T.
  • Journal Title: Cytometry A 77A Pages: 1032-1037
  • Peer Reviewed
• [Journal Article] Postpartum increase of serum thioredoxin concentrations and the relation to CD8 lymphocytes. (2010)
  • Author(s): Kuroda, S., Watanabe, M., Santo, T., Shimizuishi, Y., Takano, T., Hidaka, Y., Kimura, T., Iwatani, Y.
  • Journal Title: Ann Clin Biochem 47 Pages: 62-66
  • Peer Reviewed
• [Journal Article] mRNA quantification after FACS using in situ hybridization (2010)
  • Author(s): Yamada H, Takano T, et al.
  • Journal Title: Cytometry A Volume: 77A Pages: 1032-1037
  • Peer Reviewed
• [Journal Article] Messenger RNA quantification after fluorescence activated cell sorting using intracellular antigens. (2010)
  • Author(s): Yamada, H, Takano T, et al.
  • Journal Title: Biochem Biophys Res Commun Volume: 397 Pages: 425-428
  • Peer Reviewed
• [Journal Article] Trefoil Factor 3 (TFF3) : A promising indicator for diagnosing thyroid follicular carcinoma. (2009)
  • Author(s): Takano, T, Yamada, H.
  • Journal Title: Endocr J 56 Pages: 9-16
  • Peer Reviewed
• [Journal Article] Outpatient administration of radioactive iodine after total thyroidectomy for pediatric thyroid cancer : a report of three cases. (2009)
  • Author(s): Takano. T., Yane, K., Oue, T., Otsuki, N., Nibu, K., Hidaka, Y.
  • Journal Title: Int J Pediatr Otorhinolaryngol 73 Pages: 1810-1813
  • Peer Reviewed
• [Journal Article] Expression of nestin mRNA is a differentiation marker in thyroid tumors. (2009)
  • Author(s): Yamada, H., Takano, T., Ito, Y., Matsuzuka, F., Miya, A., Kobayashi, K., Yoshida, H., Watanabe, M., Iwatani, Y., Miyauchi, A.
  • Journal Title: Cancer Lett 280 Pages: 61-64
  • Peer Reviewed
• [Journal Article] BRAF mutation in papillary thyroid carcinoma in a Japanese population : its lack of correlation with high-risk clinicopathological features and disease-free survival of patients. (2009)
  • Author(s): Ito, Y., Yoshida, H., Maruo, R., Morita, S., Takano, T., Hirokawa, M., Yabuta, T., Fukushima, M., Inoue, H., Tomoda, C., Kihara, M., Uruno, T., Higashiyama, T., Takamura, Y., Miya, A., Kobayashi, K., Matsuzuka, F., Miyauchi, A.
  • Journal Title: Endocr J 56 Pages: 89-97
  • Peer Reviewed
• [Journal Article] Acquired activated protein C resistance is associated with IgG antibodies to protein S in patients with systemic lupus erythematosus. (2009)
  • Author(s): Nojima, J., Iwatani, Y., Ichihara, K., Tsuneoka, H., Ishikawa, T., Yanagihara, M., Takano, T., Hidaka, Y.
  • Journal Title: Thromb Res 124 Pages: 127-131
  • Peer Reviewed
• [Journal Article] Increased expression levels of tensin3 mRNA in thyroid functional adenomas as compared to non-functioning adenomas. (2009)
  • Author(s): Maeda, I., Yamada, H., Takano, T., Nishihara, E., Ito, Y., Matsuzuka, F., Miya, A., Kobayashi, K., Yoshida, H., Miyauchi, A., Hidaka, Y.
  • Journal Title: Exp Clin Endocrinol Diabetes 117 Pages: 191-193
  • Peer Reviewed
• [Journal Article] Acquired activated protein C resistance is associated with IgG antibodies to protein S in patients with systemic lupus erythematosus (2009)
  • Author(s): Nojima, J., et al.
  • Journal Title: Thromb Res 124 Pages: 127-131
  • Peer Reviewed
• [Journal Article] Increased expression levels of tensin3 mRNA in thyroid functional adenomas as compared to non-functioning adenomas (2009)
  • Author(s): Maeda, I., et al.
  • Journal Title: Exp Clin Endocrinol Diabetes 117 Pages: 191-193
  • Peer Reviewed
• [Journal Article] Expression of nestin mRNA is a differentiation marker in thyroid tumors (2009)
  • Author(s): Yamada, H., et al.
  • Journal Title: Cancer Lett 280 Pages: 61-64
  • Peer Reviewed
• [Journal Article] Outpatient administration of radioactive iodine after total thyroidectomy for pediatric thyroid cancer : a report of three cases (2009)
  • Author(s): Takano. T., et al.
  • Journal Title: Int J Pediatr Otorhinolaryngol 73 Pages: 1810-1813
  • Peer Reviewed
• [Journal Article] Trefoil Factor 3 (TFF3) : A Promising Indicator for Diagnosing Thyroid Follicular Carcinoma (2009)
  • Author(s): Takano T., Yamada H.
  • Journal Title: Endocr J (In press)
  • Peer Reviewed
• [Journal Article] Arteriosclerosis obliterans associated with anti-cardiolipin antibody/beta2-glycoprotein I antibodies as a strong risk factor for ischaemic heart disease in patients with systemic lupus erythematosus. (2008)
  • Author(s): Nojima, J., Masuda, Y., Iwatani, Y., Kuratsune, H., Watanabe, Y., Suehisa, E., Takano, T., Hidaka, Y., Kanakura, Y.
  • Journal Title: Rheumatology (Oxford) 47 Pages: 684-689
  • Peer Reviewed
• [Journal Article] Preparation of thyroid tumor cells in aspiration biopsies fro aspiration bipsy-nucleic acid diagnosis. (2008)
  • Author(s): Takano, T., Higashiyama, T., Uruno, T., Yamada, H., Yoshida, H., Miyauchi, A.
  • Journal Title: Head & Neck 30 Pages: 983-990
  • Peer Reviewed
• [Journal Article] Preparation of thyroid tumor cells in aspiration biopsiesfor aspiration biopsy nucleic acid diagnosis (2008)
  • Author(s): Takano T, et al.
  • Journal Title: Head Neek 3 0 Pages: 983-90
  • Peer Reviewed
• [Journal Article] Arteriosclerosis obliterans associated with anti-cardiolipinantibody/beta2-glycoprotein l antibodies as a strong risk factor for ischaemic heart disease in patients with systemiclupus erythematosus (2008)
  • Author(s): Noj ima J, et al.
  • Journal Title: Rheumatology (Oxford) 4 7 Pages: 684-9
  • Peer Reviewed
• [Journal Article] mRNA quantification after fluorescence activated cell sorting using locked nucleic acid probes.
  • Author(s): Maruo, R., Yamada, H., Watanabe, M., Hidaka, Y., Iwatani Y., Takano T.
  • Journal Title: Mol biotechnol in press
  • Peer Reviewed
• [Presentation] 甲状腺癌へのアプローチ:新たな発癌理論、芽細胞発癌説(fetal cell carcinogenesis)で見た甲状腺癌の臨床(Meet the Specialist) (2011)
  • Author(s): 高野徹
  • Organizer: 第20回臨床内分泌代謝Update
  • Place of Presentation: 札幌市
  • Year and Date: 2011-01-28
• [Presentation] 新たな発癌理論、芽細胞発癌説で見た甲状腺癌の臨床 (2011)
  • Author(s): 高野徹
  • Organizer: 第20回臨床内分泌代謝Update
  • Place of Presentation: 札幌市(招待講演)
  • Year and Date: 2011-01-28
• [Presentation] 新たな発癌理論、芽細胞発癌説(fetal cell carcinogenesis)の理論と実践 (2010)
  • Author(s): 高野徹
  • Organizer: 第49回日本臨床細胞学会秋季大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-11-21
• [Presentation] 細胞材料からの核酸診断-穿刺吸引核酸診断法の開発の経験から (2010)
  • Author(s): 高野徹
  • Organizer: 第49回日本臨床細胞学会秋季大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-11-21
• [Presentation] 細胞材料からの核酸診断-穿刺吸引核酸診断法の開発の経験から (2010)
  • Author(s): 高野徹
  • Organizer: 第49回日本臨床細胞学会秋季大会
  • Place of Presentation: 神戸市(招待講演)
  • Year and Date: 2010-11-21
• [Presentation] 甲状腺腫瘍に対する穿刺吸引核酸診断法の開発 (2010)
  • Author(s): 高野徹、山田宏哉、渡邊幹夫、岩谷良則、日高洋
  • Organizer: 第57回日本臨床検査医学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-11
• [Presentation] 芽細胞発癌説(fetal cell carcinogenesis)がリードする癌研究の新展開 (2009)
  • Author(s): 高野徹
  • Organizer: 第267回伊藤病院クリニカルカンファレンス
  • Place of Presentation: 東京
  • Year and Date: 2009-06-16
• [Presentation] 多段階発癌説に代わる発癌理論:芽細胞発癌説(fetal cell carcinogenesis)と癌研究の新戦略 (2009)
  • Author(s): 高野徹
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 前橋市
  • Year and Date: 2009-04-24
• [Presentation] 多段階発癌説に代わる発癌理論 : 芽細胞発癌説 (fetal cell carcinogenesis) と癌研究の新戦略 (2009)
  • Author(s): 高野徹
  • Organizer: 第82回 日本内分泌学会学術総会
  • Place of Presentation: 前橋市
  • Year and Date: 2009-04-24
• [Presentation] 新規発癌モデル、芽細胞発癌説(fetal cell carcinogenesis)と臨床検査の将来 (2009)
  • Author(s): 高野徹
  • Organizer: 第54回日本臨床検査医学会中国・四国支部総会、第149回日本臨床化学中国支部例会・総会、第19回日本臨床化学会四国支部例会・総会第5回合同地方会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-02-08
• [Presentation] シンポジウム : 新規発癌モデル、芽細胞発癌説(fetal cellcarcinogenesis)と臨床検査の将来 (2009)
  • Author(s): 高野 徹
  • Organizer: 第54回日本臨床検査医学会中国・四国支部総会
  • Place of Presentation: 岡山大学
  • Year and Date: 2009-02-08
• [Presentation] 多段階発癌説に代わる発癌理論:芽細胞発癌説(fetal cell carcinogenesis)の理論と実践(シンポジウム) (2008)
  • Author(s): 高野徹
  • Organizer: 第41回日本甲状腺外科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-16
• [Presentation] シンポジウム : 多段階発癌説に代わる発癌理論 : 芽細胞発癌説(fetalcell carcinogenesis)の理論と実践 (2008)
  • Author(s): 高野 徹
  • Organizer: 第41回日本甲状腺外科学会学術集会
  • Place of Presentation: 東京・都市センターホテル
  • Year and Date: 2008-10-16
• [Remarks] ホームページ等
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• [Patent(Industrial Property Rights)] 再構成された遺伝子の単クローン性を判定する方法、およびこれを利用した腫瘍性病変の鑑別方法 (2010)
  • Inventor(s): 高野徹
  • Industrial Property Rights Holder: 高野徹
  • Filing Date: 2010-09-17
• [Patent(Industrial Property Rights)] 甲状腺腫瘍マーカーおよび甲状腺腫瘍の分子分類法 (2009)
  • Inventor(s): 高野徹
  • Industrial Property Rights Holder: 高野徹
  • Filing Date: 2009-12-25
• [Patent(Industrial Property Rights)] 甲状腺腫瘍マーカーおよび甲状腺腫瘍の分子分類法 (2009)
  • Inventor(s): 高野徹
  • Industrial Property Rights Holder: 高野徹
  • Filing Date: 2009-12-25