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Mechanical role of tyrosine kinase PYK2 in cardiovascular stress response and therapeutic regulation of the kinase activity

Research Project

Project/Area Number 20590884
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

OKIGAKI Mituhiko  京都府立医科大学, 医学研究科, 准教授 (10333197)

Co-Investigator(Kenkyū-buntansha) 松原 弘明  京都府立医科大学, 医学研究科, 教授 (10239072)
沢田 尚久  京都府立医科大学, 医学研究科, 講師 (00315938)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsストレス / PYK2 / チロシンキナーゼ / 活性酸素 / 血管生物学 / チロシンキナーぜ
Research Abstract

We have clarified that various cardiovascular stress activates tyrosine kinase PYK2.The activated PYK2 subsequently produce Reactive Oxygen Species(ROS) production and the ROS promotes cardiovascular inflammation, leading to many cardiovascular disease such as atherosclerosis, cardiovascular remodeling after myocardial ischemia and pressure-overload by aortic banding, neointimal hypertrophy after endothelial damages. In this study, we studied whether inhibition of PYK2 in vivo may attenuate the cardiovascular diseases in mice model. We intravenously introduced PYK2-shRNA into mice to knockdown PYK2 in vivo. Expression levels of PYK2 in heart, aorta and kidney decreased less than 30% of the control. Then, 14 days after introducing shRNA for PYK2, the mice was conducted the cardiovascular injury model described above. We observed that endothelial injury by guide wire, hypercholesterolemia under Apo-E deficiency, medial hypertrophy after Angiotensin-II infusion, myocardial hypertrophy after aortic banding was markedly attenuated. Thus, PYK2 is novel and potential therapeutic target of various cardiovascular diseases.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (7 results)

All 2011 2010 2009 2008

All Journal Article (3 results) Presentation (4 results)

  • [Journal Article] Early inflammatory reactions in atherosclerosis are induced by proline-rich tyrosine kinase/reactive oxygen species-mediated release of tumor necrosis factor-alpha and subsequent activation of the p21Cip1/Ets-1/p300 system2011

    • Author(s)
      Katsume A, Okigaki M, Matsui A, Che J, Adachi Y, Kishita E, Yamaguchi S, Ikeda K, Ueyama T, Matoba S, Yamada H, Matsubara H
    • Journal Title

      Arterioscler Thromb Vasc Biol

      Volume: 31 Pages: 1084-92

    • Related Report
      2010 Final Research Report
  • [Journal Article] Endothelial FGF receptor signaling accelerates atherosclerosis2011

    • Author(s)
      Che J, Okigaki M, Takahashi T, Katsume A, Adachi Y, Yamaguchi S, Matsunaga S, Takeda M, Matsui A, Kishita E, Ikeda K, Yamada H, Matsubara H
    • Journal Title

      Am J Physiol Heart Circ Physiol

      Volume: 300 Pages: 154-61

    • Related Report
      2010 Final Research Report
  • [Journal Article] Klotho is associated with VEGF receptor-2 and the transient receptor potential canonical-1 Ca2+channel to maintain endothelial integrity2010

    • Author(s)
      Kusaba T, Okigaki M, Matui A, Murakami M, Ishikawa K, Kimura T, Sonomura K, Adachi Y, Shibuya M, Shirayama T, Tanda S, Hatta T, Sasaki S, Mori Y, Matsubara H
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 107 Pages: 19308-13

    • Related Report
      2010 Final Research Report
  • [Presentation] Aldosterone promotes vascular inflammation mediated by trans-activation of Caveolin/Angiotensin-II-Receptor-Type-1/tyrosine kinase PYK2 pathway2011

    • Author(s)
      Yamaguchi S, Matui A, Okigaki M, Fukai K, Matsubara H
    • Organizer
      American Heart Association Scientific Sessions
    • Place of Presentation
      Orland, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Non-genomic action of Aldosterone in VSMC requires translocation of Mineralocorticoid Receptor to the Caveolae in the plasma-membrane and sub-sequent trans-activation of AngiotensinII Type1 Receptor and its downstream tyrosine kinase PYK2/ROS/MAPK pathway2010

    • Author(s)
      Yamaguchi S, Matui A, Okigaki M, Matsubara H
    • Organizer
      American Heart Association Scientific Sessions
    • Place of Presentation
      Chicago, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Ca2+/Redox-Sensitive Tyrosine Kinase PYK2 Promotes Atherogenesis Through OxLDL/ROS/p21-Dependent Premature Senescence in Endothelium2009

    • Author(s)
      Katsume A, Okigaki M, Matsui A, Jisan C, Yamaguchi S, Yamada H, Matsubara H
    • Organizer
      American Heart Association Scientific Sessions
    • Place of Presentation
      Orland, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Calcium/Redox Sensitive Tyrosine Kinase Pyk2 Aggravates Myocardial Ishemia by Promoting Inflammatory Reactions and Cell Apoptosis2008

    • Author(s)
      Kishita E, Honsho S, Yamaguchi S, Katsume A, Matsui A, Matsunaga S, Okigaki M, Matsubara H
    • Organizer
      American Heart Association Scientific Sessions
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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