| Project/Area Number |
20590895
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
HOSHINO Yuma Kyoto University, 医学研究科, 助教 (00378746)
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| Co-Investigator(Kenkyū-buntansha) |
MURO Shigeo 京都大学, 医学研究科, 講師 (60344454)
ITO Isao 京都大学, 医学研究科, 助教 (40447975)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Hajime 田附興風会北野病院, 健診部長 (70303914)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 閉塞性肺疾患 / 動物モデル / COPD / 自己免疫 / Th17炎症 / 喫煙 / 慢性肺疾患 / 獲得免疫 / 慢性閉塞性肺疾患 |
|---|
| Research Abstract |
Involvement ofTh17 in the persistent neutrophil inflammation in COPD was examined using a mouse model of cigarette smoke-induced emphysema. Smoke exposure upregulated mRNA expression of interleukin-17 and increaesd Th17 cells in the lungs and spleen of "smoke-susceptible" mouse strain but not in those of "smoke-resistant" strain. To explore mechanisms of the Th17 induction by cigarette smoke, naive mice were sensitized with lung-lysate of smoke-exposed mice and challaged with cigarette smoke itself. The sensitization, however, was not sufficient for Th17 induction. We then examined adaptive transfer of splenic cells or bone marrow-derived dendritic cell exposed to cigarette smoke extract-treated lung lysate in advance. The adaptive transfer of the immune cells, however, did not induce Th17 response in the lungs of smoke-challanged mice. In conclusion, Th17 was indeed induced by cigarette smoking in mice and was related to disease susceptibility. Further studies are required to prove causal relationship between Th17 and emphysema progression.
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