Study for therapeutic effect of hepatocyte specific Pten deficiency on severe obesity.

• Project/Area Number: 20591041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Akita University
• Principal Investigator: OHSHIMA Shigetoshi Akita University, 医学部, 講師 (50375268)
• Co-Investigator(Kenkyū-buntansha): HORIE Yasuo 秋田大学, 医学部, 講師 (30282164)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: PTEN / 肥満 / 遺伝子治療 / Pten遺伝子 / ob/obマウス / Ob / マウス / Obマウス

Research Abstract

Our aim is to investigate whether hepatocyte-specific Pten deficiency can improve obesity in the leptin-deficient ob/ob mouse by activating lipogenesis in the liver. For this purpose, we have mated hepatocyte-specific Pten deficient mouse with leptin-deficient ob/ob mouse and established ob/ob/AlbCre/Pten^<flox/flox> mouse. ob/ob mice (ob/ob group), ob/ob/AlbCre/Pten^<flox/flox> mice (double KO group), AlbCre/Pten^<flox/flox> mice (KO group), Wild type mice (WT group) were sacrificed at 40 weeks just after weaning and measured body weight, fat tissue weight around testis or ovary, liver weight, triglyceride content in the liver, serum transaminase level and total ROS level in serum. Moreover we investigated Akt activation by western blot analysis and examined the expression of various genes especially related to β-oxidation by quantitative real-time PCR. The result is Hepatocyte-specific Pten deficiency improved obesity in the leptin-deficient ob/ob mouse by activating lipogenesis in the liver.

Research Products

• [Presentation] 肝細胞特異的Pten欠損によるレプチン欠損ob/obマウスの肥満の改善 (2011)
  • Author(s): 大嶋重敏,堀江泰夫,後藤隆,三浦光一,澁谷友美,佐藤亘,道免孝洋,姉崎有美子,山田育弘,鎌田健太郎,鈴木聡,大西洋英
  • Organizer: 肝臓病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-03
• [Presentation] Hepatocyte-specific Pten deficiency improves obesity in the leptin-deficient ob/ob mouse. (2011)
  • Author(s): Shigetoshi Ohshima, Yasuo Horie, Takashi Goto, Kouichi Miura, Akira Suzuki, Hirohide Ohnishi
  • Organizer: DDW2011
  • Place of Presentation: Chicago
  • Year and Date: 2011-05-10