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Study for therapeutic effect of hepatocyte specific Pten deficiency on severe obesity.

Research Project

Project/Area Number 20591041
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionAkita University

Principal Investigator

OHSHIMA Shigetoshi  Akita University, 医学部, 講師 (50375268)

Co-Investigator(Kenkyū-buntansha) HORIE Yasuo  秋田大学, 医学部, 講師 (30282164)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsPTEN / 肥満 / 遺伝子治療 / Pten遺伝子 / ob/obマウス / Ob / マウス / Obマウス
Research Abstract

Our aim is to investigate whether hepatocyte-specific Pten deficiency can improve obesity in the leptin-deficient ob/ob mouse by activating lipogenesis in the liver. For this purpose, we have mated hepatocyte-specific Pten deficient mouse with leptin-deficient ob/ob mouse and established ob/ob/AlbCre/Pten^<flox/flox> mouse. ob/ob mice (ob/ob group), ob/ob/AlbCre/Pten^<flox/flox> mice (double KO group), AlbCre/Pten^<flox/flox> mice (KO group), Wild type mice (WT group) were sacrificed at 40 weeks just after weaning and measured body weight, fat tissue weight around testis or ovary, liver weight, triglyceride content in the liver, serum transaminase level and total ROS level in serum. Moreover we investigated Akt activation by western blot analysis and examined the expression of various genes especially related to β-oxidation by quantitative real-time PCR. The result is Hepatocyte-specific Pten deficiency improved obesity in the leptin-deficient ob/ob mouse by activating lipogenesis in the liver.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (2 results)

All 2011

All Presentation (2 results)

  • [Presentation] 肝細胞特異的Pten欠損によるレプチン欠損ob/obマウスの肥満の改善2011

    • Author(s)
      大嶋重敏,堀江泰夫,後藤隆,三浦光一,澁谷友美,佐藤亘,道免孝洋,姉崎有美子,山田育弘,鎌田健太郎,鈴木聡,大西洋英
    • Organizer
      肝臓病学会総会
    • Place of Presentation
      東京
    • Year and Date
      2011-06-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Hepatocyte-specific Pten deficiency improves obesity in the leptin-deficient ob/ob mouse.2011

    • Author(s)
      Shigetoshi Ohshima, Yasuo Horie, Takashi Goto, Kouichi Miura, Akira Suzuki, Hirohide Ohnishi
    • Organizer
      DDW2011
    • Place of Presentation
      Chicago
    • Year and Date
      2011-05-10
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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