| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
We identified a novel human myeloid progenitor population that expressed Notch-1 at a high level. These Notch-1 positive progenitors preferentially differentiated into the monocyte/dendritic cell lineage in vitro. Notch-1 positive progenitors emerged in the culture of Notch-1 negative granulocyte/macrophage progenitors (GMPs), suggesting that they are more committed to the monocyte/dendritic cell lineage compared to GMPs. Indeed, they highly expressed monocyte/dendritic cell-affiliated genes including SpiB, FLT3, and PU.1. When Notch-1 positive progenitors were cultured in the presence of Notch-ligand (Delta-like 1), their progeny dendritic cells expressed lower levels of CD86 and HLA-DR compared to cells cultured without Delta-like 1, suggesting that Notch signals suppress the activation/maturation of dendritic cell lineage.
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