Development of the new diagnosis and the new therapy for rheumatoid arthritis by microRNA

• Project/Area Number: 20591171
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Kobe University
• Principal Investigator: NAKAMACHI Yuji Kobe University, 医学部附属病院, 臨床検査技師 (80379429)
• Co-Investigator(Kenkyū-buntansha): KAWANO Seiji 神戸大学, 医学部附属病院, 講師 (20351512) ; KUMAGAI Shunichi 神戸大学, 大学院・医学研究科, 教授 (00153346)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: マイクロRNA / 関節リウマチ

Research Abstract

We investigated the possibility of microRNA as a useful diagnostic marker or therapeutic drug for the treatment of rheumatoid arthritis. We used the rat adjuvant-induced arthritis model. We found that microRNA expression profile was different between the arthritis rat and non-arthritis rat at joint tissues and in mononuclear cells. We treated the adjuvant-induced arthritis rat with miR-124 by injecting into right hind heel, and the arthritis of miR-124 injected rats subsided compared to that of control rats. These results suggested that microRNA could be useful as a new diagnostic and therapueutic tool for rheumatoid arthritis.

Research Products

• [Journal Article] miR-124a as a key regulator of proliferation and MCP1 secretion in synoviocytes from RA patient. (2011)
  • Author(s): 河野誠司, 中町祐司
  • Journal Title: Ann Rheum Dis. 70 Suppl 1.
  • Peer Reviewed
• [Journal Article] 関節リウマチにおけるmicroRNAの意義と役割 (2011)
  • Author(s): 河野誠司, 中町祐司, 熊谷俊一
  • Journal Title: リウマチ科 44(1) Pages: 105-111
• [Journal Article] Quantitative Detection of PML-RARa Fusion Transcript by Real-Time PCR with A Single Primer Pair (2009)
  • Author(s): Takenokuchi M., Nakamachi Y., Yoneda K., Joo K., Kawano S., Tatsumi E., Saigo K., Kumagai S.
  • Journal Title: J Clin Lab Anal. 23 Pages: 223-230
  • Peer Reviewed
• [Journal Article] miR-124a is a key regulator of proliferation and MCP1 secretion in fibroblast like synoviocytes from patients with rheumatoid arthritis. (2009)
  • Author(s): Nakamachi Y., Kawano S., Takenokuchi M.Nishimura K.Sakai Y.Chin T.Saura R.Kurosaka M., Kumagai S.
  • Journal Title: Arthritis Rheum. 60 Pages: 1294-1304
  • Peer Reviewed
• [Journal Article] DR negativity is a distinctive feature of M1/M2 AML cases with NPM1 mutation. (2008)
  • Author(s): Syampurnawati M, Tatsumi E, Ardianto B, Takenokuchi M, Nakamachi Y, Kawano S, Kumagai S, Saigo K, Matsui T, Takahashi T, Nagai K, Gunadi, Nishio H, Yabe H, Kondo S, Hayashi Y.
  • Journal Title: Leuk Res. 32 Pages: 1141-1143
  • Peer Reviewed
• [Presentation] microRNA-124aとその両側1塩基が欠失したmicroRNA-124の生理的機能の違い (2010)
  • Author(s): 野口依子、中町祐司、河野誠司、熊谷俊一, 他
  • Organizer: 日本臨床検査医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-11
• [Presentation] マイクロRNAと自己免疫疾患 (2010)
  • Author(s): 河野誠司、中町祐司、熊谷俊一
  • Organizer: 日本臨床検査医学会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Presentation] miR-124a is a regulator of proliferation and MCP1 secretion in fibroblast like synoviocytes from patients with rheumatoid arthritis (2010)
  • Author(s): 中町祐司.河野誠司, 熊谷俊一, et al.
  • Organizer: 国際免疫学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-25
• [Presentation] 関節リウマチ特異的マイクロRNAの病態解析 (2009)
  • Author(s): 中町祐司、河野誠司、熊谷俊一, 他
  • Organizer: 日本臨床検査医学会
  • Place of Presentation: 札幌
  • Year and Date: 2009-08-28
• [Presentation] 免疫疾患の環境因子とエビジェネティクス (2009)
  • Author(s): 河野誠司、中町祐司、熊谷俊一
  • Organizer: 日本臨床検査医学会
  • Place of Presentation: 札幌
  • Year and Date: 2009-08-27
• [Presentation] miR-124aはRA滑膜細胞で細胞増殖とMCP1分泌を調整する (2009)
  • Author(s): 中町祐司、河野誠司、熊谷俊一, 他
  • Organizer: 日本リウマチ学会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-26
• [Presentation] 関節リウマチ特異的マイクロRNAの病態解析 (2009)
  • Author(s): 中町祐司
  • Organizer: リウマチフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2009-01-17
• [Presentation] miR-124a is a regulator of proliferation and MCP1 secretion in fibroblast like synoviocytes from patients with rheumatoid arthritis. (2008)
  • Author(s): 中町祐司, 河野誠司, 熊谷俊一
  • Organizer: 日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] miR-124a is a regulator of proliferation and MCP1 secretion in fibroblast like synoviocytes from patients with rheumatoid arthritis. (2008)
  • Author(s): 中町祐司, 河野誠司, 熊谷俊一, et al.
  • Organizer: ACR.
  • Place of Presentation: San Francisco
  • Year and Date: 2008-10-25
• [Patent(Industrial Property Rights)] 関節リウマチ関連マイクロRNA (2009)
  • Inventor(s): 河野誠司、中町祐司
  • Industrial Property Rights Holder: 神戸大学
  • Filing Date: 2009-02-23
• [Patent(Industrial Property Rights)] 関節リウマチ関連マイクロRNA (2008)
  • Inventor(s): 河野誠司、中町祐司
  • Industrial Property Rights Holder: 神戸大学
  • Industrial Property Number: 2008-174072
  • Filing Date: 2008-07-03