Role of ROD1 in the pathogenesis of allergic inflammation

• Project/Area Number: 20591186
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Nagoya University
• Principal Investigator: MASUDA Aklo Nagoya University, 医学系研究科, 助教 (10343203)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ROD1 / RNA / RNA結合タンパク / splicing / RODI

Research Abstract

We found that ROD1 binds to GU/GC rich regions in introns to regulate splicing. We succeeded to analyze RNA-protein interaction with CLIP method, which is a quite effective method established recently. We identified 4 isoforms of ROD1 and found difference in the ability of nuclear-cytoplasmic shuttling.

Research Products

• [Journal Article] (2010)
  • Author(s): Chiba N, Kakimoto K, Masuda A, Matsuguchi T.
  • Journal Title: Biochem Biophys Res Commun. 402 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Functional roles of Cot/Tp12 in mast cell responses to lipopolysaccharide and FcepsilonRI-clustering. (2010)
  • Author(s): Norika Chiba
  • Journal Title: Biochem Biophys Res Commun. Volume: 402 Pages: 1-6
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Ohno K, Ito M, Masuda A.
  • Journal Title: Nihon Shinkei Seishin Yakurigaku Zasshi. 29(4) Pages: 145-151
• [Journal Article] (2009)
  • Author(s): Fu Y, Ito M, Fujita Y, Ito M, Ichihara M, Masuda A, Suzuki Y, Maesawa S, Kajita Y, Hirayama M, Ohsawa I, Ohta S, Ohno K.
  • Journal Title: Neuroscience Letters 453 Pages: 81-85
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Bian Y, Masuda A, Matsuura T, Ito M, Okushin K, Engel AG, Ohno K.
  • Journal Title: Hum Mol Genet 18 Pages: 1229-1237
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Matsuguchi T, Chiba N, Bandow K, Kakimoto K, Masuda A, Ohnishi T.
  • Journal Title: J Bone Miner Res. 24(3) Pages: 398-410
  • Peer Reviewed
• [Journal Article] Molecular bases and therapeutic strategies in defective neuromuscular transmissions : lessons learned from a prototypical synapse. (2009)
  • Author(s): Kinji Ohno
  • Journal Title: Nihon Shinkei Seishin Yakurigaku Zasshi. Volume: 29 Pages: 145-151
• [Journal Article] Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson's disease. (2009)
  • Author(s): Fu Yuan
  • Journal Title: Neuroscience Letters Volume: 453 Pages: 81-85
  • Peer Reviewed
• [Journal Article] Tannic acid facilitates expression of the polypyrimidine tract binding protein and alleviates deleterious inclusion of CHRNA1 exon P3A due to an hnRNP H-disrupting mutation in congenital myasthenic syndrome. (2009)
  • Author(s): Yang Bian
  • Journal Title: Hum Mol Genet Volume: 18 Pages: 1229-1237
  • Peer Reviewed
• [Journal Article] JNK activity is essential for Atf4 expression and late-stage osteoblast differentiation. (2009)
  • Author(s): Tetsuya Matsuguchi
  • Journal Title: J Bone Miner Res. Volume: 24 Pages: 398-410
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): Masuda A, Shen X-M, Ito M, Matsuura T, Engel AG, Ohno K.
  • Journal Title: Hum Mol Genet. 17 Pages: 4022-4035
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): Ito M, Masuda A, Jinno S, Katagiri T, Krejci E, Ohno K.
  • Journal Title: Chem Biol Interact. 175 Pages: 346-348
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): Gao K, Masuda A, Matsuura T, Ohno K.
  • Journal Title: Nucleic Acids Research 36 Pages: 2257-2267
  • Peer Reviewed
• [Journal Article] Viral vector-medicated expression of human collagen Q in cultured cells (2008)
  • Author(s): Mikako Ito
  • Journal Title: Chem Biol Interact 175 Pages: 346-348
  • Peer Reviewed
• [Journal Article] Human branch point consensus sequence is yUnAy (2008)
  • Author(s): Kaiping Gao
  • Journal Title: Nucleic Acids Research 36 Pages: 2257-2267
  • Peer Reviewed
• [Journal Article] HnRNP H enhances skipping of a nonfunctional exon P3A in CHRNA1 and a mutation disrupting its binding causes congenital myas thenic syndrome (2008)
  • Author(s): Akio Masuda
  • Journal Title: Hum Mol Genet 17 Pages: 4022-4035
  • Peer Reviewed
• [Presentation] Henriette Skovgaard Andersen, Thomas Koed Doktor, Brage Storstein Andresen,大野欽司HITS-CLIP法により同定したスプライシング因子CUGBP1およびMBNL1の標的RNA配列の機能解析 (2010)
  • Author(s): 増田章男
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 一橋記念講堂(東京)
  • Year and Date: 2010-07-27
• [Presentation] HITS-CLIP法により同定したスプライシング因子CUGBP1およびMBNL1の標的RNA配列の機能解析 (2010)
  • Author(s): 増田章男
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 一橋記念講堂
  • Year and Date: 2010-07-27
• [Remarks] ホームページ等
  • URL: http://www.med.nagoya-u.ac.jp/neurogenetics/
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/neurogenetics/
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/neurogenetics/
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/neurogenetics/