| Project/Area Number |
20591283
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
KAWASAKI Yukihiko Fukushima Medical University, 医学部, 准教授 (00305369)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOYA Mitsuaki 福島県立医科大学, 医学部, 教授 (80192318)
HASHIMOTO Kouichi 福島県立医科大学, 医学部, 准教授 (50322342)
SUYAMA Kazuhide 福島県立医科大学, 医学部, 助教 (90423798)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | HUS / VEGF / Angiopoietin-1 / 糸球体内皮細胞障害 / 再生機序 / LPS / Stx / Angiopoietin 1 / 糸球体内皮細胞 / 再生 / リポポリザカライド / 腎糸球体内皮細胞 |
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| Research Abstract |
We examined the process of the renal recovery in mice with HUS and the relationship between its renal recovery and VEGF, Angiopoietin-1. C57BL/6 mice were divided into three groups. Group 1 was consisted of mice received an inyraperitoneal injection of dose of LPS at 300 μ g/kg and 225 ng/kg Stx2, Group 2 was consisted of mice received an inyraperitoneal injection of a low dose of LPS at 100 μ g/kg and 100 ng/kg Stx2. Mice in the control group were inoculated with saline. The kidneys were extirpated from 5 mice of each group sacrificed with time after administration for histological evaluation. Serum Crs in Group 1 were higher than those in Group 2 and all mice in Group 1 were died at 72-80 hours and all mice in Group 2 were alive. The fragmented erythrocytes were found in both groups. Endothelial injury and mesangiolysis scores at 24 hours in Group 1 were higher than those in Group 2. In addition, mesangial proliferation and mesangial matrix scores, CD31 positive expression at 72 hours in Group 2 were higher than those in Group 1. Glomerular VEGF and Angiopoietin-1 expressing in Group 2 gradually increased and were peak from 72 hours to 7 days. HUS mice induced by low dose of LPS and Stx2 were useful model for the recovery process of acute renal injury and VEGF and Angiopoietin-1 play important roles of its recovery process.
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