Investigation on cell specific mechanism in the tube-like differentiation in extra-villous trophoblast

• Project/Area Number: 20591301
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Kyushu University
• Principal Investigator: FUKUSHIMA Kotaro Kyushu University, 大学病院, 講師 (40304779)
• Co-Investigator(Kenkyū-buntansha): 月森 清巳 九州大学, 大学病院, 講師 (90253450)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 絨毛細胞 / 血管内皮様分化 / 低酸素 / 活性酸素種 / 酸化ストレス / 妊娠高血圧症候群 / 胎盤 / 絨毛細胞分化 / 分化 / アポトーシス / HIF1A

Research Abstract

We investigated on cell specific mechanism in the tube-like differentiation in extra-villous trophoblast. As a result, the gene expression profile in EVT during tube formation is very different from that of endothelial cells despite these cells presented morphological change. HIF1A has a crucial role in regulating EVT behavior including matrigel-induced endovascular differentiation under normoxic condition. On the contrary, ROS produced by XO in hypoxia-reperfusion induced apoptosis and affected invasion ability and differentiation in EVT. M directly injures the placental syncytiotrophoblast in PIH patients and that elevated UA in PIH patients, at least partly, indicates placental damage induced by ROS. Moreover, the role and significance of ROS injury in the placenta may differ between early-onset and late-onset type PIH.

Research Products

• [Journal Article] 8-hydroxy-2-deoxyguanosine staining in placenta is associated with maternal serum uric acid levels and gestational age at diagnosis in preeclampsia. (2011)
  • Author(s): Fukushima K, et al.
  • Journal Title: American Journal of Hypertension Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] 絨毛細胞の分化機構と妊娠高血圧症候群 (2010)
  • Author(s): 福嶋恒太郎, 村田将春, 月森清巳, 和氣徳夫
  • Journal Title: 産婦人科の実際 59巻7号 Pages: 1043-1050
• [Journal Article] 絨毛細胞の分化機構と妊娠高血圧症候群 (2010)
  • Author(s): 福嶋恒太郎, ら
  • Journal Title: 産婦人科の実際 Volume: 59 Pages: 1043-1050
• [Journal Article] Xanthine oxidaseが産生する活性酸素種はヒト不死化絨毛外絨毛細胞(EVT)由来細胞株のアポトーシスを誘導する (2009)
  • Author(s): 村田将春、福嶋恒太郎、関博之、竹田省、和氣徳夫, ほか
  • Journal Title: 日本妊娠高血圧学会雑誌なし 17 Pages: 240-241
• [Journal Article] Xanthine oxidase が産生する活性酸素種はヒト不死化絨毛外絨毛細胞(EVT)由来細胞株のアポトーシスを誘導する (2009)
  • Author(s): 村田将春, 福嶋恒太郎, 関博之, 竹田省, 和氣徳夫, ほか
  • Journal Title: 日本妊娠高血圧学会雑誌 17 Pages: 240-241
• [Journal Article] Can we diagnose invasive cervical cancer during pregnancy as precise as in non pregnant women? Maternal and perinatal outcome in pregnanties complicated with cervical cancers (2009)
  • Author(s): Fukushima K, et al
  • Journal Title: Intenational Journal of Gynocologic oncology 19 Pages: 1439-45
  • Peer Reviewed
• [Journal Article] Hypoxia Inducible Factor 1 Alpha Regulates Matrigel-induced Endovascular Differentiation under Normoxia in a Human Extravillous Trophoblast Cell Line. (2008)
  • Author(s): Fukushima K, Murata M, Hachisuga M, Tsukimori K, Seki H, Takeda S, Asanoma K, Wake N
  • Journal Title: Placenta. 29(4) Pages: 324-331
• [Journal Article] Nakano H and Wake N Neutrophil-derived Reactive Oxygen Species Can Modulate Neutrophil Adhesion to Endothelial Cells in Preeclampsia. (2008)
  • Author(s): Tsukimori K, Tsushima A, Fukushima K
  • Journal Title: Am J Hypertens 21 Pages: 587-591
• [Journal Article] Gene Expression Profiles by Microarray Analysis during Matrigel-induced Tube Formation in a Human Extravillous Trophoblast Cell line : Comparison with Endothelial Cells. (2008)
  • Author(s): Fukushima K, Murata M, Hachisuga M, Tsukimori K, Seki H, Takeda S, Kato K, Wake N
  • Journal Title: Placenta 29 Pages: 898-904
• [Journal Article] Serum uric acid levels correlate with plasma hydrogen peroxide and protein carbonyl levels in Preeclampsia. (2008)
  • Author(s): Tsukimori K, Yoshitomi T, Morokuma S, Fukushima K, Wake N
  • Journal Title: Am J Hypertens
• [Journal Article] Gene expression profiles by microarray analysis during matrige-induced tube formation in a human extravillous trophoblast cell line : comparison with endothelial cells. (2008)
  • Author(s): Fukushima K, et al.
  • Journal Title: Placenta 29 Pages: 898-904
  • Peer Reviewed
• [Journal Article] Hypoxia inducible factor 1 alpha regulates matrigel-induced endovascular differentiation under normoxia in a human extravillous trophoblast cell line. (2008)
  • Author(s): Fukushima K, et al.
  • Journal Title: Placenta 29 Pages: 324-31
  • Peer Reviewed
• [Journal Article] 8-hydroxy-2-deoxyguanosine staining in placenta is associated with maternal serum uric acid levels and gestational age at diagnosis in preeclampsia.
  • Author(s): Fukushima K, Murata M, Tsukimori K, Kaneki E, Wake N
  • Journal Title: Am J Hypertens in press
• [Presentation] Xanthine oxidaseが産生する活性酸素種はヒト不死化絨毛外絨毛細胞(EVT)由来細胞株のアポトーシスを誘導する (2010)
  • Author(s): 村田将春、福嶋恒太郎、関博之、竹田省、和氣徳夫
  • Organizer: 第17回日本胎盤学会学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2010-10-17
• [Presentation] Xanthine oxidase が産生する活性酸素種はヒト不死化絨毛外絨毛細胞(EVT)由来細胞株のアポトーシスを誘導する (2010)
  • Author(s): 村田将春, 福嶋恒太郎, 関博之, 竹田省, 和氣徳夫
  • Organizer: 第17回日本胎盤学会学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2010-10-17
• [Presentation] Xanthine oxidaseが産生する活性酸素種はヒト不死化絨毛外絨毛細胞(EVT)由来細胞株のアポトーシスを誘導する (2009)
  • Author(s): 村田将春, 福嶋恒太郎, 月森清巳, 関博之, 竹田省, 和氣徳夫
  • Organizer: 日本妊娠高血圧症候群学会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-08-29
• [Presentation] Xanthine oxidase が産生する活性酸素種はヒト不死化絨毛外絨毛細胞(EVT)由来細胞株のアポトーシスを誘導する (2009)
  • Author(s): 村田将春, 福嶋恒太郎, 月森清巳, 関博之, 竹田省, 和氣徳夫
  • Organizer: 日本妊娠高血圧症候群学会
  • Place of Presentation: 岡山市
  • Year and Date: 2009-08-29
• [Presentation] Xanthine oxidaseが産生する活性酸素種はヒト不死化絨毛外絨毛細胞(EVT)由来細胞株のアポトーシスを誘導する (2009)
  • Author(s): 村田将春, 福嶋恒太郎, 月森清巳, 関博之, 竹田省, 和氣徳夫
  • Organizer: 第61回日本産科婦人科学会総会・学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2009-04-03
• [Presentation] Xanthine oxidase が産生する活性酸素種はヒト不死化絨毛外絨毛細胞(EVT)由来細胞株のアポトーシスを誘導する (2009)
  • Author(s): 村田将春, 福嶋恒太郎, 月森清巳, 関博之, 竹田省, 和氣徳夫
  • Organizer: 第61回日本産科婦人科学会総会・学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2009-04-03
• [Presentation] 絨毛外トロフォブラストの血管内皮様分化制御機構 (2008)
  • Author(s): 福嶋恒太郎, ほか
  • Organizer: 第16回 日本胎盤学会シンポジウム
  • Place of Presentation: 浜松市
  • Year and Date: 2008-11-13
• [Presentation] (2008)
  • Author(s): 福嶋恒太郎, ほか
  • Organizer: 第16回 日本胎盤学会
  • Place of Presentation: 浜松市
  • Year and Date: 2008-11-13
• [Presentation] マイクロアレイ法を用いた胎盤絨毛外トロフォブラストの分化に関わる遺伝子のスクリーニング (2008)
  • Author(s): 村田将春、福嶋恒太郎, ほか
  • Organizer: 第60回 日本産科婦人科学会学術講演会平
  • Place of Presentation: 横浜市
  • Year and Date: 2008-04-12
• [Presentation] マイクロアレイ法を用いた胎盤絨毛外トロフォブラストの分化に関わる遺伝子のスクリーニング (2008)
  • Author(s): 村田 将春, 福嶋恒太郎, ほか
  • Organizer: 第60回 日本産科婦人科学会学術講演会
  • Place of Presentation: 横浜市
  • Year and Date: 2008-04-12
• [Remarks] ホームページ等