Elucidation of penetration mechanisms of sensory nerve fibers on a basement membrane in atopic dermatitis and application to intractable pruritus
Project/Area Number |
20591354
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Dermatology
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Research Institution | Juntendo University |
Principal Investigator |
TAKAMORI Kenji Juntendo University, 医学部, 特任教授 (40053144)
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Co-Investigator(Kenkyū-buntansha) |
TOMINAGA Mitsutoshi 順天堂大学, 医学研究科, 研究員 (50468592)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 痒み / 知覚神経 / アトピー性皮膚炎 / 細胞外マトリックス / MMP-2 / MMP-8 / 難治性痒み / 知覚神経線維 / 真皮 / Sema3A / NGF / コラーゲン / 基底膜 |
Research Abstract |
This study was performed to investigate mechanisms of penetration of sensory nerve fibres into the basement membrane of the skin. A rat dorsal root ganglion neuron culture system consisting of Matrigel and a Boyden chamber containing a nerve growth factor (NGF) concentration gradient was used, and membrane-associated MMP-2 contributes to penetration of nerve fibres into Matrigel through modulation by axonal guidance molecules and/or extracellular matrix components. Another study was performed to investigate elongation mechanisms of dermal nerve fibers within interstitial collagen matrix. Our study using the neuron culture system suggested that MMP-8 is involved in sensory nerve growth within interstitial collagen matrix.
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Report
(4 results)
Research Products
(124 results)
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[Journal Article] Antimicrobial peptides human beta-defensins and cathelicidin LL-37 induce the secretion of a pruritogenic cytokine interleukin-31 by human mast cells.2010
Author(s)
Niyonsaba F, Ushio H, Hara M, Yokoi H, Tominaga M, Takamori K, Kajiwara N, Saito H, Nagaoka I, Ogawa H, Okumura K.
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Journal Title
J Immunol. 184
Pages: 3526-34
Related Report
Peer Reviewed
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[Journal Article] Effect of a novel kappa-receptor agonist, nalfurafine hydrochloride, on severe itch in 337 haemodialysis patients : a Phase III, randomized, double-blind, placebo-controlled study.2010
Author(s)
Kumagai H, Ebata T, Takamori K, Muramatsu T, Nakamoto H, Suzuki H.
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Journal Title
Nephrol Dial Transplant. 25
Pages: 1251-1257
Related Report
Peer Reviewed
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[Journal Article] Antimicrobial peptides human beta-defensins and cathelicidin LL-37 induce the secretion of a pruritogenic cytokine interleukin-31 by human mast cells2010
Author(s)
Niyonsaba F, Ushio H, Hara M, Yokoi H, Tominaga M, Takamori K, Kajiwara N, Saito H, Nagaoka I, Ogawa H, Okumura K
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Journal Title
J Immunol
Volume: 184
Pages: 3526-3534
Related Report
Peer Reviewed
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[Journal Article] Equatorin : identification and characterization of the epitope of the MN9 antibody in the mouse.2009
Author(s)
Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Takamori K, Ogawa H, Araki Y, Miyado K, Toyama Y, Toshimori K.
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Journal Title
Biol Reprod. 81
Pages: 889-97
Related Report
Peer Reviewed
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[Journal Article] Autoantibody to NA14 is an independent marker primarily for Sjogren's syndrome.2009
Author(s)
Nozawa K, Ikeda K, Satoh M, Reeves WH, Stewart CM, Li YC, Yen TJ, Rios RM, Takamori K, Ogawa H, Sekigawa I, Takasaki Y, Chan EK.
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Journal Title
Front Biosci. 14
Pages: 3733-9
Related Report
Peer Reviewed
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[Journal Article] ABCA12 is a major causative gene for non-bullous congenital ichthyosiform erythroderma.2009
Author(s)
Sakai K, Akiyama M, Yanagi T, McMillan JR, Suzuki T, Tsukamoto K, Sugiyama H, Hatano Y, Hayashitani M, Takamori K, Nakashima K, Shimizu H.
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Journal Title
J Invest Dermatol. 129
Pages: 2306-9
Related Report
Peer Reviewed
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[Journal Article] Molecular diversity of TEX101, a marker glycoprotein for germ cells monitored with monoclonal antibodies : Variety of the molecular characteristics according to subcellular localization within the mouse testis.2008
Author(s)
Yoshitake H, Shirai Y, Mochizuki Y, Iwanari H, Tsubamoto H, Koyama K, Tominaga M, Ogawa H, Hasegawa A, Kodama T, Hamakubo T, Araki Y.
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Journal Title
J Reprod Immunol. 79
Pages: 1-11
Related Report
Peer Reviewed
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[Journal Article] Protein biomarker analysis by mass spectrometry in patients with rheumatoid arthritis receiving anti-tumor necrosis factor-alpha antibody therapy.2008
Author(s)
Sekigawa I, Yanagida M, Iwabuchi K, Kaneda K, Kaneko H, Takasaki Y, Jung G, Sone S, Tanaka Y, Ogawa H, Tominaga M
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Journal Title
Clin Exp Rheumatol. 26
Pages: 261-7
Related Report
Peer Reviewed
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[Journal Article] Involvement of very long fatty acid-containing lactosylceramide in lactosylceramide-mediated superoxide generation and migration in neutrophils.2008
Author(s)
Iwabuchi K, Prinetti A, Sonnino S, Mauri L, Kobayashi T, Ishii K, Kaga N, Murayama K, Kurihara H, Nakayama H, Yoshizaki F, Takamori K, Ogawa H, Nagaoka I.
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Journal Title
Glycoconj J. 5
Pages: 357-74
Related Report
Peer Reviewed
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