Physiology and application of deimination of humnan hair follicle S100A3

• Project/Area Number: 20591358
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kinki University
• Principal Investigator: KAWADA Akira Kinki University, 医学部, 教授 (90160986)
• Co-Investigator(Kenkyū-buntansha): TAKAHARA Hidenari 茨城大学, 農学部, 教授 (30122063)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: S100A3,脱イミノ化反応 / ヒト毛嚢 / peptidylarginine deimase / 線構造解析 / カルシウム結合タンパク質 / S100A3 / Ca^<2+>結合蛋白質 / ヒト毛嚢キューテクル / 脱イミノ化 / 結晶化 / X線 / シトルリン残基 / ヒト毛嚢キューティクル / 架橋反応 / トランスグルタミナーゼ / 蛍光分光分析 / 小角散乱 / シトルリン残

Research Abstract

S100A3 protein specifically expressed in the cuticular cells of hair follicle. Human S100A3 is characterized by two disulphide bridges, a preformed Zn^<2+>-binding site, and a specific citrullination site by deimination. Conversion to the citrulline residues, symmetrically located on the S100A3 dimer plane-causes the assembly of the Ca^<2+>-bound S100A3 homotetramer. S100A3 acts as a Ca^<2+> buffering protein providing Ca^<2+> ions in turn to the deimination.

Research Products

• [Journal Article] Transcriptional regulation of peptidylarginine deiminase expression in human keratinocytes (2009)
  • Author(s): S.Ying, S.Dong, A.Kawada, T.Kojima, S.Chavanas, M.C.Mechin, V.Adoue, G.Serre, M.Simon, H.Takahara
  • Journal Title: J.Dermatol.Sci. 53 Pages: 2-9
  • NAID: 10025331723
  • Peer Reviewed
• [Journal Article] Transcriptional Regulation of Peptidylargine Deiminase Expression in Keratinocytes (2009)
  • Author(s): Ying S
  • Journal Title: J. Dermatol. Sci. 53 Pages: 2-9
  • Peer Reviewed
• [Journal Article] Specific citrullination causes assembly of a globular S100A3 homotetramer : a putative Ca2+ modulator matures human hair cuticle. (2008)
  • Author(s): K.Kizawa, H.Takahara, H.Troxler, P.Kleinert, U.Mochida, C.W.Heizmann
  • Journal Title: J.Biol.Chem. 283 Pages: 5004-5013
  • Peer Reviewed
• [Journal Article] Specific Citrullination Causes Assembly of a Globular SI00A3 Homotetramer : A Putative Ca2+-Modulator Matures Human Hair Cuticle (2008)
  • Author(s): Kizawa K
  • Journal Title: J. Biol. Chem. 283 Pages: 5004-5013
  • Peer Reviewed
• [Journal Article] An intronic enhancer driven by NF-_KB contributes to transcriptional regulation of peptidylarginine deiminase type I gene in human keratinocytes.
  • Author(s): S.Ying, T.Kojima, A.Kawada, R Nachat, G.Serre, M.Simon, H Takahara
  • Journal Title: J Invest Dermatol. 201
  • Peer Reviewed
• [Journal Article] Refined crystal structure of human Ca^2+/Zn^<2+>-binding S100A3 protein characteraized by two disulphide bridges
  • Author(s): M.Unno, T.Kawasaki, H.Takahara, C.W.Heizmann, K.Kizawa
  • Journal Title: J.Mol.Biol. (In press)
  • Peer Reviewed