Development of a method for simultaneous multiple thoracic and abdominal organ procurement using ET-Kyoto solution

• Project/Area Number: 20591522
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyoto University
• Principal Investigator: ZHAO Xiangdong Kyoto University, 大学院・医学研究科, 助教 (00444464)
• Co-Investigator(Kenkyū-buntansha): FUJINAGA Takuji 京都大学, 大学院・医学研究科, 助教 (00444456) ; CHEN Fengshi 京都大学, 大学院・医学研究科, 助教 (00452334) ; BANDO Toru 京都大学, 大学院・医学研究科, 准教授 (20293954) ; DATE Hiroshi 京都大学, 大学院・医学研究科, 教授 (60252962)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 臓器保存 / 移植・再生医療 / トランスレーショナルリサーチ / 外科

Research Abstract

Currently, different preservation solutions are utilized for the procurement and preservation of different organs. In the present study, a novel method was developed by using ET-Kyoto solution (ET-K) alone for multiple organs. Rat heart, kidney and small bowel transplantation models were established and the methods for flushing and procurement of both thoracic and abdominal organs were discussed. The efficacy of ET-K on kidney and heart preservation was evaluated using rat transplantation models. In the renal preservation, ET-K has shown superior to HTK and comparable to UW. In the cardiac preservation, the effect of ET-K was equal to that of HTK. Furthermore, the left ventricular function was well preserved by ET-K as evaluated with Langendorff rat heart perfusion model.

Research Products

• [Journal Article] Comprehensive and innovative techniques for liver transplantation in rats : a surgical guide. (2010)
  • Author(s): Hori T, Nguyen JH, Zhao X, Ogura Y, Hata T, Yagi S, Chen F, Baine AM, Ohashi N, Eckman CB, Herdt AR, Egawa H, Takada Y, Oike F, Sakamoto S, Kasahara M, Ogawa K, Hata K, Iida T, Yonekawa Y, Sibulesky L, Kuribayashi K, Kato T, Saito K, Wang L, Torii M, Sahara N, Kamo N, Sahara T, Yasutomi M, Uemoto S.
  • Journal Title: World J Gastroenterol. 16(25) Pages: 3120-3132
  • Peer Reviewed
• [Journal Article] The generation of donor-specific CD4+CD25++CD45RA+ naive regulatory T cells in operationally tolerant patients after pediatric living-donor liver transplantation. (2010)
  • Author(s): Nafady-Hego H, Li Y, Ohe H, Zhao X, Satoda N, Sakaguchi S, Wood K, Uemoto S, Koshiba T.
  • Journal Title: Transplantation 90(12) Pages: 1547-1555
  • Peer Reviewed
• [Journal Article] Requirement of protocol biopsy before and after complete cessation of immunosuppression following liver transplantation. (2009)
  • Author(s): Yoshitomi M, Koshiba T, Haga H, Li Y, Zhao X, Cheng D, Miyagawa A, Sakashita H, Tsuruyama T, Ueda M, Okamoto S, Wood K, Sakaguchi S, Manabe T, Uemoto S.
  • Journal Title: Transplantation 87(4) Pages: 606-614
  • Peer Reviewed
• [Journal Article] Nebulized phosphodiesterase III inhibitor during warm ischemia attenuates pulmonary ischemia-reperfusion injury. (2009)
  • Author(s): Zhang J, Chen F, Zhao X, Aoyama A, Okamoto T, Fujinaga T, Shoji T, Sakai H, Cui Y, Bando T, Date H.
  • Journal Title: J Heart Lung Transplant. 28(1) Pages: 79-84
  • Peer Reviewed
• [Journal Article] Successful sub-zero non-freezing preservation of rat lungs at -2 degrees C utilizing a new supercooling technology. (2008)
  • Author(s): Okamoto T, Nakamura T, Zhang J, Aoyama A, Chen F, Fujinaga T, Shoji T, Hamakawa H, Sakai H, Manabe T, Wada H, Date H, Bando T.
  • Journal Title: J Heart Lung Transplant. 27(10) Pages: 1150-7
  • Peer Reviewed
• [Journal Article] Value of FOXP3 expression in peripheral blood as rejection marker after miniature swine lung transplantation. (2008)
  • Author(s): Satoda N, Shoji T, Wu Y, Fijinaga T, Chen F, Aoyama A, Zhang J, Takahashi A, Okamoto T, Matsumoto I, Sakai H, Li Y, Zhao X, Manabe T, Kobayashi E, Sakaguchi S, Wada H, Uemoto S, Tottori J, Bando T, Date H, Koshiba T.
  • Journal Title: J Heart Lung Transplant 27(12) Pages: 1293-1301
  • Peer Reviewed
• [Journal Article] ET-Kyoto solution plus Dibutyryl Adenosine Monophosphate is Superior to University of Wisconsin Solution in Rat Liver Preservation. (2008)
  • Author(s): Zhao X, Koshiba T, Nakamura T, Tsuruyama T, Li Y, Bnado T, Wada H, Tanaka K.
  • Journal Title: Cell Transplant 17 Pages: 99-109
  • Peer Reviewed
• [Journal Article] Value of FOXP3 Expression in Perpheral Blood as Rejection Marker Afer Miniature Swihe Lung Transplantation (2008)
  • Author(s): Naoki Satoda
  • Journal Title: The Journal of Heart and Lung Transplantation 27 Pages: 1293-1301
  • Peer Reviewed
• [Presentation] ET-Kyoto液を使用した肺移植症例の検討 (2011)
  • Author(s): 藤永卓司、中島大輔、阪本仁、趙向東、佐藤寿彦、陳豊史、庄司剛、園部誠、阪井宏彰、板東徹、伊達洋至
  • Organizer: 京都臓器保存セミナー
  • Place of Presentation: 京都市
  • Year and Date: 2011-03-19
• [Presentation] ET-Kyoto液の開発と臓器機能保存学講座5年の歩み。 (2011)
  • Author(s): 板東徹、和田洋巳、中村隆之、藤永卓司、陳豊史、趙向東、大角明宏、中島大輔、阪本仁、庄司剛、阪井宏彰、伊達洋至
  • Organizer: 京都臓器保存セミナー
  • Place of Presentation: 京都
  • Year and Date: 2011-03-19
• [Presentation] Prolonged Rat Kidney Preservation with ET-Kyoto Solution : Comparison with Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solutions. (2011)
  • Author(s): Zhao X, Fujinaga T, Li Y, Bando T, Wada H, Date H
  • Organizer: American Transplant Congress 2011
  • Place of Presentation: Philadelphia, PA, USA
• [Presentation] 臓器保存の現状と将来の展望肺移植における肺保存の現状と新たな肺保存方法の検討 (2010)
  • Author(s): 藤永卓司, 中島大輔, 阪本仁, 趙向東, 陳豊史, 庄司剛, 阪井宏彰, 板東徹, 伊達洋至
  • Organizer: 第37回日本臓器保存生物医学会学術集会
  • Place of Presentation: 新潟市
  • Year and Date: 2010-11-20
• [Presentation] ラット腎保存におけるET-Kyoto液とhistidine-tryptophan-ketoglutarate液およびUniversity of Wisconsin液の比較 (2010)
  • Author(s): 趙向東, 藤永卓司, 陳豊史, 板東徹, 和田洋巳, 伊達洋至
  • Organizer: 第46回日本移植学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-22
• [Presentation] ラット腎保存におけるET-Kyoto液とhistidine-tryptophan-ketoglutarate液及びUniversity of Wisconsin液の比較 (2010)
  • Author(s): 趙向東
  • Organizer: 第46回日本移植学会総会
  • Place of Presentation: みやこめっせ(京都市)(招待講演)
  • Year and Date: 2010-10-22
• [Remarks]
  • URL: http://www.thoracic-kyoto-u.gr.jp/
• [Remarks]
  • URL: http://www.thoracic-kyoto-u.gr.jp/
• [Remarks]
  • URL: http://www.thoracic-kyoto-u.gr.jp/