Experimental pancreatic cancer model using in vivo imaging system and its application

• Project/Area Number: 20591556
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Nippon Medical School
• Principal Investigator: UCHIDA Eiji Nippon Medical School, 大学院・医学研究科, 教授 (70176684)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 膵癌 / 実験モデル / イメージング

Research Abstract

We developed short-term pancreatic cancer transplantation models in hamsters using PGHAM-1-Luc, which is luciferase-positive PGHAM-1 cells. Administration of luciferin and usage of IVIS Imaging System could provide us with PGHAM-1 tumor imaging without sacrifice. This system might be useful not only for the research on tumor invasion and metastasis but also for the application to therapeutic trial with new substances. However, decrease of luciferase activity with culture passage is a problem to be solved.

Research Products

• [Journal Article] Nestin expression correlates with nerve and retroperitoneal tissue invasion in pancreatic cancer. (2009)
  • Author(s): M.Kawamoto, T.Ishiwata, K.Cho, E.Uchida, M.Korc, Z.Naito, T.Tajiri
  • Journal Title: Human Pathol. 40 Pages: 189-198
  • Peer Reviewed
• [Journal Article] Nestin expression correlates with nerve and retroperitoneal tissue invasion in pancreatic cancer (2009)
  • Author(s): M.Kawamoto
  • Journal Title: Human Pathol. 40 Pages: 189-198
  • Peer Reviewed
• [Journal Article] Experimental pancreatic cencer model using PGHAM-1 cells : Characteristics and experimental therapeutic trials. (2008)
  • Author(s): E.Uchida, A.Matsushita, K.Yanagi, M.Hiroi, T.Aimoto, Y.Nakamura, T.Yokoyama, T.Takashi
  • Journal Title: J.Nippon Med.School 75 Pages: 325-331
  • Peer Reviewed
• [Journal Article] Experimental pancreatic cancer model using PGHAM-1 cells : Characteristies and experimental therapeutic trials (2008)
  • Author(s): E. Uchida
  • Journal Title: J Nippon Med Sch 75 Pages: 325-331
  • Peer Reviewed
• [Presentation] Hamster pancreatic cancer model for research on metastasis and therapeutic trials (2009)
  • Author(s): 内田英二
  • Organizer: 40^<th> Anniversary Joint Meeting of the American Pancreatic Association & Japan Pancreas society.
  • Place of Presentation: Honolulu (Hawaii)
  • Year and Date: 2009-11-04
• [Presentation] Hamster pancreatic cancer model for research on metastasis and therapeutictrials (2009)
  • Author(s): E.Uchida
  • Organizer: 40^<th> Anniversary Joint Meeting of the American Pancreatic Association & Japan Pancreas society
  • Place of Presentation: Honolulu (Hawaii)
  • Year and Date: 2009-11-04
• [Presentation] イメージングシステムを用いたハムスター膵がんモデル (2008)
  • Author(s): 内田英二、相本隆幸、中村慶春、張一光、川本聖郎、山初和也、廣井信、田尻孝、島田隆
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] イメージングシステムを用いたハムスター膵がんモデル (2008)
  • Author(s): 内田英二
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28