Isolation and characterization of murine hepatic stem cells (HPCs) based on a new surface antigen.

• Project/Area Number: 20591610
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto University
• Principal Investigator: YASUCHIKA Kentaro Kyoto University, 医学研究科, 病院特定助教 (00378895)
• Co-Investigator(Kenkyū-buntansha): IKAI Iwao 京都大学, 医学研究科, 非常勤講師 (60263084) ; UEMOTO Shinji 京都大学, 医学研究科, 教授 (40252449) ; KAMO Naoko 京都大学, 医学研究科, 医員 (50452355) ; ISHII Takamichi 京都大学, 医学研究科, 医員 (70456789)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 肝幹細胞 / 表面抗原 / gp38

Research Abstract

Previously, we clarified the surface antigen profiles of hepatic progenitor cells (HPCs) in fetal liver tissue as the CD49f+/CD45-/Thyl- cell fraction. In this study, we represented that more immature HPCs could be detected by using a novel surface antigen gp38 which was expressed in fetal livers but not in adult livers. Furthermore, the characterization of gp38-positive HPCs revealed that activation of Wnt signal pathway was related to the maintenance the undifferentiated state of HPCs.

Research Products

• [Journal Article] A transmembrane glycoprotein, gp38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers (2011)
  • Author(s): 小西小百合、安近健太郎、石井隆道、福光剣、加茂直子、藤田直哉、猪飼伊和夫、上本伸二
  • Journal Title: In Vitro Cell.Dev.Biol.Animal Vol.47 Pages: 45-53
  • Peer Reviewed
• [Journal Article] A transmembrane glycoprotein, gp 38, is a novel marker for immature hepatic progenitor cells in fetal mouse livers. (2011)
  • Author(s): 小西小百合、安近健太郎, ほか
  • Journal Title: In vitro Cellular & Developmental biology.Animal Volume: 47 Pages: 45-53
  • Peer Reviewed
• [Journal Article] In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers (2010)
  • Author(s): 石井隆道、安近健太郎、福光剣、猪飼伊和夫以下7名
  • Journal Title: Cell Tissue Research Vol.339、No.3 Pages: 505-512
  • NAID: 120002511339
  • Peer Reviewed
• [Journal Article] In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers. (2010)
  • Author(s): 石井隆道、安近健太郎, ほか
  • Journal Title: Cell Tissue Research Volume: 339 Pages: 505-512
  • NAID: 120002511339
  • Peer Reviewed
• [Presentation] Variation in hepatic differentiation propensity among human induced pluripotent stem cell (iPSC) lines (2010)
  • Author(s): 梶原正俊、石井隆道、猪飼伊和夫、上本伸二、山中伸弥
  • Organizer: The 8th International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: San Francisco, U.S.A.
• [Presentation] Comparative study of transplantation of cells at various differentitation stages into mice with induced lethal liver damage (2010)
  • Author(s): 上村良、石井隆道、安近健太郎、猪飼伊和夫
  • Organizer: 第45回欧州肝臓学会議(EASL)
  • Place of Presentation: ウィーン、オーストリア
• [Presentation] Isolation of hepatic stem cells using novel surface antigen ; gp38 (2008)
  • Author(s): Konishi S, et. al
  • Organizer: 43th European association for the study of the liver
  • Place of Presentation: Milan, Italy
  • Year and Date: 2008-04-24