Myocardial protection for normothermic ischemia and reperfusion in immature heart : effect of mitochondria permeability transition pore inhibitor.

• Project/Area Number: 20591659
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Nippon Medical School
• Principal Investigator: IMURA Hajime 日本医科大学, 医学部, 准教授 (40281422)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 未熟心筋 / ミトコンドリア / 心筋保護 / MPTP阻害剤 / プロポフォール / 小児開心術 / ランゲンドルフ / 開心術 / 虚血 / 再還流障害 / MPTP / 人工心肺

Research Abstract

Propofol, which is widely used as an anesthetic agent, inhibits the mitochondria permeability transition pore(MPTP) during reperfusion after ischemia and may work for myocardial protection. In the present study, we investigated whether propofol would protect the immature heart during normothermic ischemia and reperfusion. The study was carried out by using immature rabbit hearts in the Langendorff model. Propofol showed protective effect at concentrations of 1-4μg/ml, and the best protection was at 2μg/ml. The protection at 2μg/ml was equal to that of cyclosporine(another MPTP inhibitor) in a viewpoint of functional recovery of the heart. However, we also observed significant negative inotropic effect at 4μg/ml or higher concentrations and this side effect was crucial at 10μg/ml or higher.