Novel cancer related genes of lung based on the genomic and epigenomic aberrations.

• Project/Area Number: 20591664
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: YOKOI Sana Tokyo Medical and Dental University, がんゲノムセンター, 部長 (30372452)
• Co-Investigator(Renkei-kenkyūsha): INAZAWA Johji 東京医科歯科大学, 難治疾患研究所, 教授 (30193551)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肺がん / ゲノム / DNAコピー数 / 癌 / マイクロアレイ / 肺癌 / 遺伝子増幅 / エピゲノム / ホモ欠失 / メチル化 / 免疫沈降 / X染色体

Research Abstract

To explore novel lung cancer related genes, genomic and epigenomic aberrations were detected by DNA microarray in lung cancer cell lines and primary lung tumors from Japanese patients. We detected novel lung cancer related gene in the genomic amplified regions. We also make genetically modified mice of the gene.

Research Products

• [Journal Article] Detection of Epithelial Growth Factor Receptor Mutations in Cerebrospinal Fluid from Patients with Lung Adenocarcinoma Suspected of Neoplastic Meningitis. (2011)
  • Author(s): Shingyoji M, Kageyama H, Sakaida T, Nakajima T, Matsui Y, Itakura M, Iuchi T, Yokoi S, Kimura H, Iizasa T
  • Journal Title: J Thorac Oncol. (in press)
• [Journal Article] Genome-wide DNA methylation profiles in renal tumors of various histological subtypes and non-tumorous renal tissues. (2011)
  • Author(s): Arai E, Wakai-Ushijima S, Fujimoto H, Hosoda F, Shibata T, Kondo T, Yokoi S, Imoto I, Inazawa J, Hirohashi S, Kanai Y
  • Journal Title: Pathobiology. 78 Pages: 1-9
• [Journal Article] Diagnosis and prognostication of ductal adenocarcinomas of the pancreas based on genome-wide DNA methylation profiling by bacterial artificial chromosome array-based methylated CpG island amplification. (2011)
  • Author(s): Gotoh M, Arai E, Wakai-Ushijima S, Hiraoka N, Kosuge T, Hosoda F, Shibata T, Kondo T, Yokoi S, Imoto I, Inazawa J, Kanai Y
  • Journal Title: J Biomed Biotechnol. 2011 Pages: 780836-780836
• [Journal Article] Copy number alterations in urothelial carcinomas : Their clinicopathological significance and correlation with DNA methylation alterations. (2011)
  • Author(s): Nishiyama N, Arai E, Nagashio R, Fujimoto H, Hosoda F, Shibata T, Tsukamoto T, Yokoi S, Imoto I, Inazawa J, Kanai Y
  • Journal Title: Carcinogenesis. 32 Pages: 462-9
• [Journal Article] Genome-wide DNA methylation profiles in renal tumors of various histological subtypes and non-tumorous renal tissues. (2011)
  • Author(s): Arai E
  • Journal Title: Pathobiology Volume: 78 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Diagnosis and prognostication of ductal adenocarcinomas of the pancreas based on genome-wide DNA methylation profiling by bacterial artificial chromosome array-based methylated CpG island amplification. (2011)
  • Author(s): Gotoh M
  • Journal Title: J Biomed Biotechnol Volume: 201 Pages: 780836-780836
  • Peer Reviewed
• [Journal Article] Copy number alterations in urothelial carcinomas : Their clinicopathological significance and correlation with DNA methylation alterations. (2011)
  • Author(s): Nishiyama N
  • Journal Title: Carcinogenesis Volume: 32 Pages: 462-469
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation profiles in urothelial carcinomas and urothelia at the precancerous stage. (2010)
  • Author(s): Nishiyama N, Arai E, Chihara Y, Fujimoto H, Hosoda F, Shibata T, Kondo T, Tsukamoto T, Yokoi S, Imoto I, Inazawa J, Hirohashi S, Kanai Y
  • Journal Title: Cancer Sci. 101 Pages: 231-240
  • NAID: 10027200383
• [Journal Article] Genome-wide DNA methylation profiles in urothelial carcinomas and urothelia at the precancerous stage (2010)
  • Author(s): Nishiyama N
  • Journal Title: Cancer Sci. 101 Pages: 231-240
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation profiles in liver tissue at the precancerous stage and in hepatocellular carcinoma. (2009)
  • Author(s): Arai E, Ushijima S, Gotoh M, Ojima H, Kosuge T, Hosoda F, Shibata T, Kondo T, Yokoi S, Imoto I, Inazawa J, Hirohashi S, Kanai Y
  • Journal Title: Int J Cancer. 125 Pages: 2854-2862
• [Journal Article] Genome-wide DNA methylation profiles in both precancerous conditions and clear cell renal cell carcinomas are correlated with malignant potential and patient outcome. (2009)
  • Author(s): Arai E., Ushijima S., Fujimoto H., Hosoda F., Shibata T., Kondo T., Yokoi S, Imoto I, Inazawa J, Hirohashi S, Kanai Y
  • Journal Title: Carcinogenesis. 30 Pages: 214-21
• [Journal Article] Genome-wide DNA methylation profiles in liver tissue at the precancerous stage and in hepatocellular carcinoma (2009)
  • Author(s): Arai E
  • Journal Title: Int J Cancer. 125 Pages: 2854-2862
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation profiles in both precancerousconditions and clear cell renal cell carcinomas are correlatedwith malignant potential and patient outcome (2009)
  • Author(s): Arai E
  • Journal Title: Carcinogenesis 30 Pages: 214-221
  • Peer Reviewed
• [Journal Article] 遺伝子異常の全ゲノム探索 (2008)
  • Author(s): 横井左奈、稲澤譲治
  • Journal Title: 肺癌 66 Pages: 45-49
• [Journal Article] 遺伝子異常の全ゲノム探索 (2008)
  • Author(s): 横井左奈
  • Journal Title: 日本臨林 増刊 肺癌 66 Pages: 45-49
• [Presentation] 癌性髄膜炎が疑われた肺癌症例における髄液中EGFR変異の検討 (2010)
  • Author(s): 新行内雅斗
  • Organizer: 第51回日本肺癌学会総会
  • Place of Presentation: 広島
• [Presentation] バンクーバー (2010)
  • Author(s): CHEST
  • Place of Presentation: カナダ
• [Presentation] EGFR, K-ras, p53 gene mutation analysis in NSCLC using microsamples obtained by EBUS-TBNA (2010)
  • Author(s): Nakajima T
  • Organizer: CHEST
  • Place of Presentation: バンクーバー、カナダ
• [Presentation] The tripartite motif 33 (TRIM33), an amplification target for 1p13, promoted growth of breast cancer (2009)
  • Author(s): 横井左奈
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Presentation] Genome-wide DNA methylation profiles in urothelial carcinomas and urothelia at the precancerous stage (2009)
  • Author(s): 西山直隆
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Presentation] Genome-wide DNA methylation profiles in liver tissue at the precancerous stage and in hepatocellular carcinoma (2009)
  • Author(s): 新井恵吏
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Presentation] The tripartite motif 33(TRIM33), an amplification target for 1p13, promoted growth of breast cancer (2009)
  • Author(s): 横井左奈
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Presentation] 1p13増幅領域の標的遺伝子tripartite motif 33(TRIM33)は乳癌の予後不良因子である (2009)
  • Author(s): 横井左奈
  • Organizer: 日本人類遺伝学会第54回大会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-25
• [Presentation] 1p13増幅領域の標的遺伝子 tripartite motif 33(TRIM33)は乳癌の予後不良因子である (2009)
  • Author(s): 横井左奈
  • Organizer: 日本人類遺伝学会 第54回大会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-25
• [Presentation] The tripartite motif 33 (TRIM33) gene is probable target for 1p13 amplification in small cell lung cancer (2008)
  • Author(s): 横井左奈
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] X-tiling アレイを用いたX染色体上の癌関連遺伝子群の探索 (2008)
  • Author(s): 坂本宙子
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] The tripartite motif 33 (TRIM33)gene is probable target forlpl3 amplification in small cell lung cancer (2008)
  • Author(s): 横井左奈
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] X-tilingアレイを用いたX染色体上の癌関連遺伝子群の探索 (2008)
  • Author(s): 坂本宙子
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] アレイCGH 法による肺小細胞癌の潜在的ゲノムコピー数解析と癌関連遺伝子の同定 (2008)
  • Author(s): 横井左奈
  • Organizer: 日本人類遺伝学会第53回大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-29
• [Presentation] アレイCGH法による肺小細胞癌の潜在的ゲノムコピー数解析と癌関連遺伝子の同定 (2008)
  • Author(s): 横井左奈
  • Organizer: 日本人類遺伝学会 第53回大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-09-29
• [Presentation] レット症候群の自閉症状の要因としてのシナプス関連分子のエピジェネテックな制御異常 (2008)
  • Author(s): 久保田健夫
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] レッド症候群の自閉症状の要因としてのシナプス関連分子の工ピジェネテックな制御異常 (2008)
  • Author(s): 久保田健夫
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] EGFR, K-ras, p53 gene mutation analysis in NSCLC using microsamples obtained by EBUS-TBNA
  • Author(s): Nakajima T