| Project/Area Number |
20591735
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Yamagata University |
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Principal Investigator |
HASHIMOTO Junichi (2009-2010) Yamagata University, 医学部, 講師 (10359565)
高原 政利 (2008) Yamagata University, 医学部, 准教授 (10236341)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGI Michiaki 山形大学, 医学部, 准教授 (40241707)
KIKUCHI Noriaki 山形大学, 医学部, 助教 (90361261)
WATANABE Tadayoshi 山形大学, 医学部, 助教 (90422170)
TABATA Yasuhiko 京都大学, 再生医科学研究所, 教授 (50211371)
NARITA Atsushi 山形大学, 医学部, 医員 (60400579)
橋本 淳一 山形大学, 医学部, 助教 (10359565)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 筋 / 神経病学 / 再生医学 / 生体材料 |
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| Research Abstract |
The aim of this study was to determine the appropriate concentration of platelet rich plasma (PRP) for a bone regeneration and evaluate the effect of PRP on MSCs. In in vitro study, we showed that PRP had a concentration-dependent effect on the cell proliferation. MSCs differentiated into osteoblasts with 125x10^4/μl and 62.5x10^4/ μl of PRP. In in vivo study, adequate bone regeneration was observed using 8x10^6 of MSCs and 2x10^8 of platelet. PRP could be candidate for safe and useful activator in clinical application.
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