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Experimental study for anterior cruciate ligament using cell sheet technology

Research Project

Project/Area Number 20591747
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionTokai University

Principal Investigator

MITANI Genya  Tokai University, 医学部, 講師 (80307280)

Co-Investigator(Kenkyū-buntansha) SATOU Masato  東海大学, 医学部, 准教授 (10056335)
KUTUNA Toshiharu  東海大学, 医学部, 講師 (90328120)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsACL / 細胞シート / 再生医療 / 前十字靭帯 / Tenomodulin / 前十字靱帯
Research Abstract

Triple-layered ACL cell sheets and SCL-derived cell sheets were fabricated successfully without enzymatic digestion. Furthermore, 3-dimensional bioengineered ACL was fabricated by a combination of triple-layered ACL cell sheets and bioabsorbable mesh composite. Immunohistochemical examination and real-time PCR revealed that TnmD is a specific marker of the human ACL, and ACL sheets have a similar phenotype to the ACL. The superior expression of TnmD in synovial tissues, and synovium-derived cell sheets indicates that synovium is a potential cell source for ACL regeneration.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (10 results)

All 2011 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (8 results)

  • [Journal Article] The properties of bioengineered chondrocyte sheets for cartilage regeneration BMC biotechnology 20092009

    • Author(s)
      Genya Mitani, Masato Sato, Jeong IK Lee1, Nagatoshi Kaneshiro, Miya Ishihara, Naoshi Ota, Mami Kokubo, Hideaki Sakai,Tetsutaro Kikuchi, Joji Mochida
    • Related Report
      2010 Final Research Report
  • [Journal Article] The properties of bioengineered chondrocyte sheets for cartilage regeneration2009

    • Author(s)
      G. Mitani
    • Journal Title

      BMC Biotechnology 2009vol. 9, 17

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 再生医療に向けた前十字靱帯、滑膜由来細胞シートの検討2011

    • Author(s)
      三谷玄弥
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      京王プラザホテル(東京)
    • Year and Date
      2011-03-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト膝関節内組織におけるTenomodulin、Scleraxisの発現状況2011

    • Author(s)
      三谷玄弥
    • Organizer
      第10回日本再生医療学会総会
    • Related Report
      2010 Final Research Report
  • [Presentation] 再生医療に向けた前十字靱帯、滑膜由来細胞シートの検討2010

    • Author(s)
      三谷玄弥
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Place of Presentation
      京都国際会議場(京都)
    • Year and Date
      2010-10-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 1.ヒト膝関節内組織におけるTenomodulin、Scleraxisの発現状況2010

    • Author(s)
      三谷玄弥
    • Organizer
      1.2^<nd> JOSKAS
    • Place of Presentation
      沖縄コンベンションセンター(沖縄)
    • Year and Date
      2010-07-03
    • Related Report
      2010 Annual Research Report
  • [Presentation]2010

    • Author(s)
      三谷玄弥
    • Organizer
      第25回日本整形外科学会基礎学術集会
    • Related Report
      2010 Final Research Report
  • [Presentation] JOSKAS ヒト膝関節内組織におけるTenomodulin、Scleraxisの発現状況2010

    • Author(s)
      三谷玄弥
    • Related Report
      2010 Final Research Report
  • [Presentation] ヒト前十字靭帯細胞シート、滑膜組織におけるTenomodulinの発現状況2009

    • Author(s)
      三谷玄弥
    • Organizer
      第23回日本整形外科学会基礎学術集会(再生医療に向けた前十字靱帯、滑膜由来細胞シートの検討)
    • Related Report
      2010 Final Research Report
  • [Presentation] ヒト前十字靭帯細胞シート、滑膜組織におけるTenomodulinの発現状況2008

    • Author(s)
      三谷 玄弥
    • Organizer
      第23回日本整形外科学会基礎学術集会
    • Place of Presentation
      京都
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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