Functional analysis of polycomb proteins and target molecules in rheumatoid arthritis pathogenesis
| Project/Area Number |
20591786
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
KOMIYA Setsuro (2009-2010) Kagoshima University, 医歯学総合研究科, 教授 (30178371)
有島 善也 (2008) Kagoshima University, 大学院・医歯学総合研究科, 客員研究員 (90336339)
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| Co-Investigator(Kenkyū-buntansha) |
SETOGUCHI Takao 鹿児島大学, 医学部・歯学部附属病院, 助教 (40423727)
ARISHIMA Yoshiya 鹿児島大学, 医学部・歯学部附属病院, 助教 (90336339)
小宮 節郎 鹿児島大学, 大学院・医歯学総合研究科, 教授 (30178371)
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| Research Collaborator |
NAGAO Hiroko 鹿児島大学, 医歯学総合研究科, 大学院生
SASAKI Hiromi 鹿児島大学, 医歯学総合研究科, 大学院生
MATSUNOSHITA Yukihiro 鹿児島大学, 医歯学総合研究科, 大学院生
KUNIGOU Osamu 鹿児島大学, 医歯学総合研究科, 大学院生
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ポリコーム蛋白 / EZH2,BMI-1 / 関節リウマチ / 骨肉腫 / BMI-1 / EZH2 |
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| Research Abstract |
Expression of EZH2 in synovial cells of rheumatoid arthritis and osteosarcoma cells was increased. BMI-1 was also up-regulated in synovial cells of rheumatoid arthritis and osteosarcoma cells. Expression of EZH2 and BMI-1 was up-regulated in osteosarcoma patient' biopsy specimens. Immunohistochemical examinations showed that EZH2 and BMI-1 were up-regulated in osteosarcoma cells and that trimethylation of histone H3K27 was increased. Unexpectedly, knock down of EZH2 and BMI-1 prevented osteosarcoma growth neither in vitro nor in vivo. Our findings suggest that EZH2 and BMI-1 may be a antigen of rheumatoid arthritis or osteosarcoma, but are not useful molecular targets of treatment.
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Report
(4 results)
Research Products
(37 results)
