Treatment of ischemic neuronal damage by the preservation of mitochondrial membrane potential

• Project/Area Number: 20591804
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Okayama University
• Principal Investigator: MORITA Kiyoshi Okayama University, 大学院・医歯薬学総合研究科, 教授 (40108171)
• Co-Investigator(Kenkyū-buntansha): TAKEDA Yoshimasa 岡山大学, 岡山大学病院, 講師 (30294466)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 蘇生学 / ミトコンドリア / 脳虚血

Research Abstract

The present research revealed following phenomenon.
1. Potassium channel opener decreases mitochondrial membrane potential to 51% of control level in rats (in vivo).
2. Concentration gradient of potassium is one of the most important factors that generate mitochondrial membrane potential.
3. Attenuation of mitochondrial potential does not activate glycolysis.
4. Attenuation of potassium gradient in mitochondria does not affect energy production in rat brain (in vivo).

Research Products

• [Journal Article] Dynamic changes in cortical NADH fluorescence in rat focal ischemia : Evaluation of the effects of hypothermia on propagation of peri-infarct depolarization by temporal and spatial analysis (2009)
  • Author(s): Toshihiro Sasaki, Yoshimasa Takeda, Hideki Taninishi, Minako Arai, Kensuke Shiraishi, Kiyoshi Morita
  • Journal Title: Neuroscience Letters 449 Pages: 61-65
  • Peer Reviewed
• [Journal Article] Dynamic changes in cortical NADH fluorescence in rat focal ischemia : Evaluation of the effects of hypothermia on propagation of peri-infarct depolarization by temporal and spatial analysis (2009)
  • Author(s): I Sasaki, Y laKeda, H Taninishi, M Arai, K Shiraishi, K Morita
  • Journal Title: Neuroscience Letters 449 Pages: 61-65
  • Peer Reviewed
• [Presentation] ラット脳部分虚血における低体温による再発性脱分極の伝播様式に対する影響(NADH蛍光画像を用いた検討) (2008)
  • Author(s): 佐々木俊弘
  • Organizer: 麻酔科学会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-12
• [Presentation] 低体温による再発性脱分極の発生・伝播に対する影響のNADH蛍光画像を用いた検討 (2008)
  • Author(s): 佐々木俊弘
  • Organizer: 神経麻酔集中治療研究会
  • Place of Presentation: 新潟
  • Year and Date: 2008-04-12