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Quantitative detection and identification of a wide range of bacteria and fungi composing vaginal flora without culture.

Research Project

Project/Area Number 20591923
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionFukushima Medical University

Principal Investigator

SUZUTANI Tatsuo  Fukushima Medical University, 医学部, 教授 (40196895)

Co-Investigator(Kenkyū-buntansha) SATO Akira  福島県立医科大学, 医学部, 教授 (30004948)
ISHIOKA Ken  福島県立医科大学, 医学部, 助教 (50305356)
KANEKO Hisatoshi  福島県立医科大学, 医学部, 助教 (90360007)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords感染症 / 細菌 / 菌類 / 検査法 / 常在細菌叢 / 腟正常細菌叢 / 細菌性腟症 / カンジダ性腟症 / t-RLFP / real-time PCR / 正常菌叢 / 定量的菌種同定法 / サブトラクション法 / ゲノム
Research Abstract

There are many species of microbes that can not be cultured in media ; therefore, many infectious diseases have been left without diagnosis. For the identification of infectious agents, novel identification methods based on PCR without culture have been reported in the field of bacteriology. In this study, we developed a novel identification method for fungi. Using these examination techniques for bacteria and fungi, we analyzed vaginal swabs taken from patients with informed consent. The results showed that there were no correlations between the number of Candida in the samples and symptoms or the diagnosis of Candida vaginitis. Moreover, bacterial flora and number of fungi in the vagina were found to fluctuate independently.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (11 results)

All 2010 2009 2008 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (6 results)

  • [Journal Article] Cutaneous cryptococcosis in a patient with cirrhosis and hepatitis C virus infection.2010

    • Author(s)
      Miura T, Kawakami Y, Otsuka M, Hachiya M, Yamanoi T, Ohashi K, Suzutani T, Yamamoto T.
    • Journal Title

      Acta Derm Venereol

      Volume: 90 Pages: 106-107

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cutaneous cryptococcosis in a patient with cirrhosis and hepatitis C virus infection.2010

    • Author(s)
      Miura T, Kawakami Y, Otsuka M, Hachiya M, Yamanoi T, Ohashi K, Suzutani T, Yamamoto T.
    • Journal Title

      Acta Derm Venereol 90

      Pages: 106-107

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An improved rapid quantitative detection and identification method for a wide range of fungi.2009

    • Author(s)
      Soeta N, Terashima M, Gotoh M, Mori S, Nishiyama K, Ishioka K, Kaneko H, Suzutani T.
    • Journal Title

      J Med Microbiol 58

      Pages: 1037-1044

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] An improved rapid quantitative detection and identification method for a wide range of fungi.2009

    • Author(s)
      Soeta N, Terashima M, Gotoh M, Mori S, Nishiyama K, Ishioka K, Kaneko H, Suzutani T.
    • Journal Title

      J. Med. Microbiol. (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Childhood tinea incognito caused by Trichophyton mentagrophytes var . interdigitale mimicking pustular psoriasis.

    • Author(s)
      Kawakami Y, Oyama N, Sakai E, NishiyamaK, Suzutani T, Yamamoto T.
    • Journal Title

      Pediatr Dermatol in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] 正常細菌叢中に存在する真菌の新しい定量法2009

    • Author(s)
      岡崎友亮、西山恭子、石岡 賢、金子久俊、錫谷達夫
    • Organizer
      第63回日本細菌学会東北支部総会
    • Place of Presentation
      盛岡
    • Year and Date
      2009-08-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 培養によらない分子生物学的手法を用いた腟常在細菌叢の検討2009

    • Author(s)
      西山恭子、岡崎友亮、石岡 賢、金子久俊、錫谷達夫
    • Organizer
      第63回日本細菌学会東北支部総会
    • Place of Presentation
      盛岡
    • Year and Date
      2009-08-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 正常菌叢中に存在する真菌の新しい定量法2009

    • Author(s)
      岡崎友亮、西山恭子、石岡賢、金子久俊、錫谷達夫
    • Organizer
      日本細菌学会東北支部総会
    • Place of Presentation
      岩手県歯科医師会館(岩手県)
    • Year and Date
      2009-08-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] 培養によらない分子生物学的手法を用いた膣常在菌叢の検討2009

    • Author(s)
      西山恭子、岡崎友亮、石岡賢、金子久俊、錫谷達夫
    • Organizer
      日本細菌学会東北支部総会
    • Place of Presentation
      岩手県歯科医師会館(岩手県)
    • Year and Date
      2009-08-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] 培養によらない細菌・真菌の定量的検査法~産婦人科領域での応用~2008

    • Author(s)
      錫谷達夫
    • Organizer
      第540回日本産婦人科学会宮城地方部会集会
    • Place of Presentation
      仙台市(特別講演)
    • Year and Date
      2008-12-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 培養によらない細菌・真菌検査2008

    • Author(s)
      錫谷達夫
    • Organizer
      第125回日本産婦人科学会東北連合地方部会学術集会
    • Place of Presentation
      福島市(特別講演)
    • Year and Date
      2008-06-08
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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