Proteomic prediction of radiotherapy outcome in cervical cancer

• Project/Area Number: 20591956
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: University of the Ryukyus
• Principal Investigator: ASATO Tsuyoshi University of the Ryukyus, 大学院・医学研究科, 助教 (40253950)
• Co-Investigator(Renkei-kenkyūsha): NAGAI Yutaka 琉球大学, 医学部附属病院, 講師 (70305209) ; INAMINE Morihiko 琉球大学, 医学研究科, 助教 (90437989) ; KARIYA Ken-Ichi 琉球大学, 医学研究科, 教授 (40263371)
• Research Collaborator: YAMASHIRO Yoshito 琉球大学, 医学研究科・博士課程, 日本学術振興会特別研究員
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 子宮頸癌 / 放射線治療 / 子宮頚癌

Research Abstract

The present study was conducted in order to obtain data that serve as a basis for developing novel molecular markers for prediction of the outcome of radiotherapy in patients with cervical cancer. Subjects were selected from cases that were sensitive or resistant to the concurrent chemoradiotherapy (CCRT). Comprehensive protein expression profiles of pre-threapy biopsy specimen were compared between the sensitive and resistant cases. Eight proteins were identified that were expressed only in sensitive cases, while 18 proteins were identified that were expressed only in resistant cases.

Research Products

• [Journal Article] Ectopic coexpression of keratin 8 and 18 promotes invasion of transformed keratinocytes and is induced in patients with cutaneous squamous cell carcinoma. (2010)
  • Author(s): Yoshito Yamashiro, Kimiko Takei, Masato Umikawa, Tsuyoshi Asato, Minoru Oshiro, Yukiko Uechi, Takahiro Ishikawa, Kiyohito Taira, Hiroshi Uezato, Ken-ichi Kariya
  • Journal Title: Biochemical and Biophysical Research Communications 399 Pages: 365-372
  • Peer Reviewed
• [Journal Article] Impact of human papillomavirus genotype on response to treatment and survical in patients receiving radiotherapy for squamous cell carcioma of the cervix. (2010)
  • Author(s): Jannatul Ferdousi, Yutaka Nagai, Tsuyoshi Asato, Makoto Hirakawa, Morihiko Inamine, Wataru Kudaka, Ken-ichi Kariya, Yoichi Aoki
  • Journal Title: Experimental and Therapeutic Medicine 1 Pages: 525-530
  • Peer Reviewed
• [Journal Article] Ectopic coexpression of keratin 8 and 18 promotes invasion of transformed keratinocytes and is induced in patients with cutaneous squamous cell carcinoma. (2010)
  • Author(s): Yamashiro, Y., et al.
  • Journal Title: Biochem Biophys Res Commun Volume: 399 Pages: 365-372
  • Peer Reviewed
• [Journal Article] Impact of human papillomavirus genotype on response to treatment and survical in patients receiving radiotherapy for squamous cell carcioma of the cervix. (2010)
  • Author(s): Ferdousi, J., et al.
  • Journal Title: Exp Therap Med Volume: 1 Pages: 525-530
  • Peer Reviewed
• [Journal Article] Rap2 function requires palmitoylation and recycling endosome localization. (2009)
  • Author(s): Yukiko Uechi, Maitsetseg Bayarjargal, Masato Umikawa, Minoru Oshiro, Kimiko Takei, Yoshito Yamashiro, Tsuyoshi Asato, Shogo Endo, Ryo Misaki, Tomohiko Taguchi, Ken-ichi Kariya
  • Journal Title: Biochemical and Biophysical Research Communications 378 Pages: 732-737
  • Peer Reviewed
• [Journal Article] Rap2 function requires palmitoylation and recycling endosome localization. (2009)
  • Author(s): Uechi Y, et al
  • Journal Title: Biochemical and Biophysical Research Communications 378 Pages: 732-737
  • Peer Reviewed
• [Journal Article] MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. (2008)
  • Author(s): Hideo Nonaka, Kimiko Takei, Masato Umikawa, Minoru Oshiro, Kouichi Kuninaka, Maitsetseg Bayarjargal, Tsuyoshi Asato, Yoshito Yamashiro, Yukiko Uechi, Shogo Endo, Tatsuo Suzuki, Ken-ichi Kariya
  • Journal Title: Biochemical and Biophysical Research Communications 377 Pages: 573-578
  • Peer Reviewed
• [Journal Article] MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. (2008)
  • Author(s): Nonaka H, et al
  • Journal Title: Biochemical and Biophysical Research Communications 377 Pages: 573-578
  • Peer Reviewed
• [Presentation] The proteomic search for prognosis markers of cutaneous squamous cell carcinoma. (2009)
  • Author(s): Yoshito Yamashiro (代表発表者), Kimiko Takei, Masato Umikawa, Tsuyoshi Asato, Kiyohito Taira, Hiroshi Uezato, Ken-ichi Kariya
  • Organizer: MBSJ2009(第32回日本分子生物学会年会)プログラム:3P-0743 : 418
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] プロテオミクス解析による有棘細胞癌予後マーカーの探索 (2009)
  • Author(s): 山城義人, 他
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] Localization of Rap2 in recycling endosome in a palmitoylationdependent manner. (2008)
  • Author(s): Maitsetseg Bayarjargal (代表発表者), Yukiko Uechi, Shogo Endo, Kimiko Nonaka-Takei, Minoru Oshiro, Masato Umikawa, Tsuyoshi Asato, Yoshito Yamashiro, Tomohiko Taguchi, Ken-ichi Kariya
  • Organizer: Biochemistry Molecular Biology (BMB) 2008(第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会)プログラム:1P-0444 : 219
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-09
• [Presentation] Localization of Rap2 in recycling endosome in a palmitoylation-dependent manner. (2008)
  • Author(s): Bayarjargal M, et al
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 兵庫県、神戸国際展示場
  • Year and Date: 2008-12-09