New therapeutic approach for nasopharyngeal carcinoma using induction of Epstein-Barr virus lytic cycle by anti-cancer chemotherapy

• Project/Area Number: 20592012
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kanazawa University
• Principal Investigator: MURONO Shigeyuki Kanazawa University, 附属病院, 講師 (20345622)
• Co-Investigator(Renkei-kenkyūsha): YOSHIZAKI Tomokazu 金沢大学, 医学系, 教授 (70262582) ; WAKISAKA Naohiro 金沢大学, 附属病院, 講師 (70377414) ; KONDO Satoru 金沢大学, 附属病院, 助教 (70436822)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 上咽頭癌 / 抗癌剤 / EBウイルス / 複製サイクル / ガンシクロビル

Research Abstract

Treatment of Epstein-Barr virus-positive epithelial cell lines with CDDP, one of anti-cancer drugs, induced BZLF1 protein, which play an important role in switching latent EBV infection to lytic cycle. No clear induction of apoptosis by the drug was observed. In the biopsy specimens taken from post-chemotherapy nasopharyngeal carcinoma patients, histological effect of chemotherapy was grade 0 or 1. On the other hand, immunohistochemistry for BZLF1 could not be properly performed.

Research Products

• [Journal Article] Current understanding and management of nasopharyngeal carcinoma (2011)
  • Author(s): Yoshizaki T, Ito M, Murono S, Wakisaka N, Kondo S, Endo K
  • Journal Title: Auris Nasus Larynx in press
  • NAID: 120003959461
  • Peer Reviewed
• [Journal Article] Epstein-Barr virus latent membrane protein 1 induces Snail and epithelial-mesenchymal transition in metastatic nasopharyngeal carcinoma (2011)
  • Author(s): Horikawa T, Yoshizaki T, Kondo S, Furukawa M, Kaizaki Y
  • Journal Title: British Journal of Cancer 104 Pages: 1160-1167
  • Peer Reviewed
• [Journal Article] EBウイルスに着目した上咽頭癌の診断と治療 (2010)
  • Author(s): 室野重之, 吉崎智一
  • Journal Title: 耳鼻と臨床 56
  • NAID: 130004555460
  • Peer Reviewed
• [Journal Article] EBウイルスに着目した上咽頭癌の診断と治療 (2010)
  • Author(s): 室野重之, ほか
  • Journal Title: 耳鼻と臨床 Volume: 56(補1)
  • NAID: 130004555460
• [Journal Article] Treatment of nasopharyngeal carcinoma : Treatment management and future view of Epstein-Barr virus-targeting treatment (2009)
  • Author(s): Murono S, Kondo S, Wakisaka N, Yoshizaki T
  • Journal Title: Current Cancer Therapy Reviews 5 Pages: 170-177
  • Peer Reviewed
• [Journal Article] Phosphorylated ezrin is associated with EBV latent membrane protein 1 in nasopharyngeal carcinoma and induces cell migration (2009)
  • Author(s): Endo K, Kondo S, Shackleford J, Horikawa T, Kitagawa N, Yoshizaki T, Furukawa M, Zen Y, Pagano JS
  • Journal Title: Oncogene 28 Pages: 1725-1735
  • Peer Reviewed
• [Journal Article] Induction of receptor for advanced glycation end products by EBV latent membrane protein 1 and its correlation with angiogenesis and cervical lymph node metastasis in nasopharyngeal carcinoma (2008)
  • Author(s): Tsuji A, Wakisaka N, Kondo S, Murono S, Furukawa M, Yoshizaki T
  • Journal Title: Clinical Cancer Research 14 Pages: 5368-5375
  • NAID: 120001130604
  • Peer Reviewed
• [Presentation] 当科における上咽頭癌症例の検討 (2010)
  • Author(s): 加藤理紗, 遠藤一平, 近藤悟, 脇坂尚宏, 室野重之, 吉崎智一
  • Organizer: 第34回日本頭頸部癌学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-06-11
• [Presentation] EBVにより上咽頭癌幹細胞は誘導されるか-第二報- (2010)
  • Author(s): 近藤悟, 北川典子, 広田京子, 脇坂尚宏, 遠藤一平, 室野重之, 吉崎智一
  • Organizer: 第34回日本頭頸部癌学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-06-11
• [Presentation] 舌癌のリンパ管新生に関する免疫組織学的研究 (2010)
  • Author(s): 広田京子, 脇坂尚宏, 近藤悟, 室野重之, 吉崎智一
  • Organizer: 第34回日本頭頸部癌学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2010-06-10
• [Presentation] EBVにより上咽頭癌幹細胞は誘導されるか-第一報- (2010)
  • Author(s): 近藤悟, 脇坂尚宏, 遠藤一平, 室野重之, 吉崎智一
  • Organizer: 第111回日本耳鼻咽喉科学会総会
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2010-05-20
• [Presentation] EBウイルスに着目した上咽頭癌の診断と治療 (2010)
  • Author(s): 室野重之, 吉崎智一
  • Organizer: 第15回頭頸部癌化学療法研究会
  • Place of Presentation: ホテルセントラーザ博多(福岡県)
  • Year and Date: 2010-03-06