Immunological properties of cancer stem cells in squamous cell carcinoma of the head and neck

• Project/Area Number: 20592013
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: University of Yamanashi
• Principal Investigator: CHIKAMATSU Kazuaki University of Yamanashi, 医学部附属病院, 講師 (30301378)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 癌 / 免疫学 / 癌幹細胞 / 頭頸部扁平上皮癌 / CD44 / アポトーシス / 免疫抑制 / 免疫抑制機構 / 治療抵抗性

Research Abstract

Recent progress in the understanding of stem cell biology has greatly facilitated the identification and characterization of cancer stem cells (CSCs). In squamous cell carcinoma of the head and neck (SCCHN), CD44+ population has been demonstrated to possess the properties of CSCs. In this study, I compared the immunological properties of CD44+ cancer stem-like cells with CD44- cells. CD44+ cells have higher malignant potential with numerous escape strategies from immune attack. Moreover, CD44+ cells were also resistant to various apoptosis-inducing stimuli.

Research Products

• [Journal Article] Resistance to apoptosis-inducing stimuli in CD44+ head and neck squamous cell carcinoma cells. (2011)
  • Author(s): Chikamatsu K, Ishii H, Takahashi, G, Okamoto A, Moriyama M, Sakakura K, Masuyama K.
  • Journal Title: Head Neck (in press
  • Peer Reviewed
• [Journal Article] Immune regulation by CD4+CD25+ regulatory T cells in patients with Japanese cedar pollinosis. (2011)
  • Author(s): Yamanishi T, Chikamatsu K, Takahashi G, Endo S, Masuyama K.
  • Journal Title: Int Arch Allergy Immunol. (in press)
  • Peer Reviewed
• [Journal Article] Immunoregulatory properties of CD44+ cancer stem-like cells in squamous cell carcinoma of the head and neck. (2011)
  • Author(s): Chikamatsu K, Takahashi G, Sakakura K, Ferrone S, Masuyama K.
  • Journal Title: Head Neck 33 Pages: 208-215
  • Peer Reviewed
• [Journal Article] Resistance to apoptosis-inducing stimuli in CD44+ head and neck squamous cell carcinoma cells. (2011)
  • Author(s): Chikamatsu K, et al.
  • Journal Title: Head Neck Volume: (in press)
  • Peer Reviewed
• [Journal Article] Immunoregulatory properties of CD44+ cancer stem-like cells in squamous cell carcinoma of the head and neck. (2011)
  • Author(s): Chikamatsu K, et al.
  • Journal Title: Head Neck Volume: 33 Pages: 208-215
  • Peer Reviewed
• [Journal Article] Primary tumor induces sentinel lymph node lymphangiogenesis in oral squamous cell carcinoma. (2010)
  • Author(s): Ishii H, Chikamatsu K, Sakakura K, Miyata M, Furuya N, Masuyama K.
  • Journal Title: Oral Oncol. 46 Pages: 373-378
  • Peer Reviewed
• [Journal Article] Primary tumor induces sentinel lymph node lymphangiogenesis in oral squamous cell carcinoma. (2010)
  • Author(s): Ishii H, et al.
  • Journal Title: Oral Oncol Volume: 46 Pages: 373-378
  • Peer Reviewed
• [Journal Article] Immunoregulatory properties of CD44+cancer stem-like cells in squamous cell carcinoma of the head and neck. (2010)
  • Author(s): Chikamatsu K, et al.
  • Journal Title: Head Neck (In press)
  • Peer Reviewed
• [Journal Article] Primary tumor induces sentinel lymph node lymphangiogenesis in oral squamous cell carcinoma. (2010)
  • Author(s): Ishii H, et al.
  • Journal Title: Oral Oncol (In press)
  • Peer Reviewed
• [Journal Article] CD4+ T-cell responses to HLA-DP5-restricted wild-type sequence p53 peptides in patients with head and neck cancer. (2009)
  • Author(s): Chikamatsu K, Sakakura K, Takahashi G, Okamoto A, Furuya N, Whiteside TL, DeLeo AB, Masuyama K.
  • Journal Title: Cancer Immunol Immunother. 58 Pages: 1441-1448
  • Peer Reviewed
• [Journal Article] Expansion and characterization of cancer stem-like cells in squamous cell carcinoma of the head and neck. (2009)
  • Author(s): Chikamatsu K, Sakakura K, Hatsushika K, Takahashi G, Masuyama K.
  • Journal Title: Oral Oncol. 45 Pages: 633-639
  • Peer Reviewed
• [Journal Article] Analysis of helper T cell responses to Cry j 1-derived peptides in patients with nasal allergy : candidate for peptide-based immunotherapy of Japanese cedar pollinosis. (2009)
  • Author(s): Masuyama K, Chikamatsu K, Ikagawa S, Matsuoka T, Takahashi G, Yamamoto T, Endo S.
  • Journal Title: Allergol Int. 58 Pages: 63-70
  • NAID: 10025109090
  • Peer Reviewed
• [Journal Article] Expansion and characterization of cancer stem-like cells in squamous cell carcinoma of the head and neck. (2009)
  • Author(s): Okamoto A, et al.
  • Journal Title: Oral Oncol 45 Pages: 633-639
  • Peer Reviewed
• [Journal Article] CD4+ T-cell responses to HLA-DP5-restricted wild-type sequence p53 peptides in patients with head and neck cancer. (2009)
  • Author(s): Chikamatsu K, et al.
  • Journal Title: Cancer Immunol Immunother (In press)
  • Peer Reviewed
• [Journal Article] Expansion and characterization of cancer stem-like cells in squamous cell careinoma of the head and neck. (2009)
  • Author(s): Okamoto A, et al.
  • Journal Title: Oral Oncol (In press)
  • Peer Reviewed
• [Journal Article] Analysis of T helper responses and FOXP3 gene expression in patients with Japanese cedar pollinosis. (2008)
  • Author(s): Chikamatsu K, Sakakura K, Matsuoka T, Endo S, Takahashi G, Matsuzaki Z, Masuyama K.
  • Journal Title: Am J Rhinol. 22 Pages: 582-588
  • Peer Reviewed
• [Journal Article] Congenital laryngeal anomalies presenting as chronic stridor : A retrospective study of 55 patients. (2008)
  • Author(s): Sakakura K, Chikamatsu K, Toyoda M, Kaai M, Yasuoka Y, Furuya N.
  • Journal Title: Auris Nasus Larynx. 35 Pages: 527-533
  • Peer Reviewed
• [Journal Article] CD4+ T helper responses in squamous cell carcinoma of the head and neck. (2008)
  • Author(s): Chikamatsu K, Sakakura K, Yamamoto T, Furuya N, Whiteside TL, Masuyama K.
  • Journal Title: Oral Oncol. 44 Pages: 870-877
  • Peer Reviewed
• [Journal Article] CD4+ T helper responses in squamous cell carcinoma of the head and neck. (2008)
  • Author(s): Chikamatsu K, et al.
  • Journal Title: Oral Oncol 44 Pages: 870-877
  • Peer Reviewed
• [Presentation] 頭頸部扁平上皮癌患者の免疫抑制に及ぼすmyeloid-derived suppressor cellの役割. (2011)
  • Author(s): 近松一朗, 坂倉浩一, 山本卓典, 森山元大, 増山敬祐.
  • Organizer: 第29回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 大分
• [Presentation] 頭頸部扁平上皮癌における癌抗原CSPG4の発現と抗原特異的免疫応答の誘導. (2011)
  • Author(s): 坂倉浩一, 近松一朗.
  • Organizer: 第29回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 大分
• [Presentation] Immunosuppressive properties of CD44+ cancer stem-like cells in squamous cell carcinoma of the head and neck. (2010)
  • Author(s): Chikamatsu K, Ishii H, Takahashi G, Sakakura K, Ferrone S, Masuyama K.
  • Organizer: 4^<th> World Congress of International Federation of Head and Neck Oncologic Societies.
  • Place of Presentation: Seoul, Korea
• [Presentation] Primary tumor induces sentinel lymph node lymphangiogenesis in oral squamous cell carcinoma. (2010)
  • Author(s): 石井裕貴, 近松一朗, 坂倉浩一, 宮田政則,古屋信彦, 増山敬祐.
  • Organizer: 4^<th> World Congress of International Federation of Head and Neck Oncologic Societies.
  • Place of Presentation: Seoul, Korea
• [Presentation] 口腔癌におけるリンパ節転移メカニズムの検討:原発巣とセンチネルリンパ節におけるリンパ管増生の関係. (2010)
  • Author(s): 近松一朗, 石井裕貴, 宮田政則, 坂倉浩一, 古屋信彦, 増山敬祐.
  • Organizer: 第111回日本耳鼻咽喉科学会総会
  • Place of Presentation: 仙台
• [Presentation] Expression of VEGF-C in oral squamous cell carcinoma correlates with sentinel lymph node lymphangiogenesis. (2010)
  • Author(s): 石井裕貴, 近松一朗, 坂倉浩一, 宮田政則,古屋信彦, 増山敬祐.
  • Organizer: American Head and Neck Society 2010 Annual Meeting.
  • Place of Presentation: Las Vegas, USA.
• [Presentation] CD44+ cancer stem-like cells are responsible for treatment resistance in squamous cell carcinoma of the head and neck. (2010)
  • Author(s): 近松一朗, 石井裕貴, 岡本篤司, 高橋吾郎, 坂倉浩一, 増山敬祐.
  • Organizer: American Head and Neck Society 2010 Annual Meeting.
  • Place of Presentation: Las Vegas, USA.
• [Presentation] Immunoregulatory properties of CD44+ cancer stem-like cells in squamous cell carcinoma of the head and neck. (2010)
  • Author(s): Chikamatsu K, Takahashi G, Sakakura K, Ferrone S, Masuyama K
  • Organizer: 101^<st> Annual Meeting American Association for Cancer Research.
  • Place of Presentation: Washington DC, USA
• [Presentation] Tumor microenvironmentにおけるCD44+ cancer stem ce11の免疫抑制機構に果たす役割. (2010)
  • Author(s): 近松一朗, 石井裕貴, 高橋吾郎, 坂倉浩一, 増山敬祐.
  • Organizer: 第28回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 福井
• [Presentation] CD44+ cencer stem cellは様々なアポトーシス誘導刺激に対して抵抗性を示す. (2010)
  • Author(s): 石井裕貴, 近松一朗, 高橋吾郎, 坂倉浩一, 増山敬祐.
  • Organizer: 第28回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 福井
• [Presentation] Immunosuppressive properties of CD44+ cancer stem-like cells in squamous cell carcinoma of the head and neck. (2010)
  • Author(s): Chikamatsu K, et al.
  • Organizer: 4^<th> World Congress of International Federation of Head and Neck Oncologic Societies
  • Place of Presentation: Seoul, Korea
• [Presentation] CD44+ cancer stem-like cells are responsible for treatment resistance in squamous cell carcinoma of the head and neck. (2010)
  • Author(s): Chikamatsu K, et al.
  • Organizer: American Head and Neck Society 2010 Annual Meeting
  • Place of Presentation: Las Vegas, USA
• [Presentation] Immunoregulatory properties of CD44+ cancer stem-like cells in squamous cell carcinoma of the head and neck. (2010)
  • Author(s): Chikamatsu K, et al.
  • Organizer: 101^<st> Annual Meeting American Association for Cancer Research
  • Place of Presentation: Washington DC, USA
• [Presentation] Tumor microenvironmentにおけるCD44+ cancer stem cellの免疫抑制機構に果たす役割. (2010)
  • Author(s): 近松一郎, 他
  • Organizer: 第28回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 福井
• [Presentation] Immunological properties of CD44+ cancer stem cells in squamous cell carcinoma of the head and neck. (2009)
  • Author(s): 近松一朗, 岡本篤司, 坂倉浩一, 初鹿恭介, 高橋吾郎, 増山敬祐.
  • Organizer: 2^<nd> World Congress of the International Academy of Oral Oncology.
  • Place of Presentation: Toronto, Canada
• [Presentation] Cancer stem cells as mediators of treatment resistance in squamous cell carcinoma of the head and neck. (2009)
  • Author(s): 岡本篤司, 近松一朗, 坂倉浩一, 初鹿恭介, 高橋吾郎, 増山敬祐.
  • Organizer: 2^<nd> World Congress of the International Academy of Oral Oncology.
  • Place of Presentation: Toronto, Canada
• [Presentation] 頭頸部扁平上皮癌における癌幹細胞の免疫抑制機構に果たす役割. (2009)
  • Author(s): 近松一朗, 坂倉浩一, 増山敬祐.
  • Organizer: 第13回日本がん免疫学会
  • Place of Presentation: 北九州
• [Presentation] 頭頸部扁平上皮癌における治療抵抗性細胞としての癌幹細胞の解析. (2009)
  • Author(s): 近松一朗, 高橋吾郎, 増山敬祐.
  • Organizer: 第33回日本頭頸部癌学会
  • Place of Presentation: 札幌
• [Presentation] Expansion and characterization of CD44+ cancer stem-like cells in squamous cell carcinoma of the head and neck. (2009)
  • Author(s): 近松一朗, 岡本篤司, 坂倉浩一, 初鹿恭介, 高橋吾郎, 増山敬祐.
  • Organizer: 100^<th> Annual Meeting American Association for Cancer Research.
  • Place of Presentation: Denver, USA
• [Presentation] 頭頸部扁平上皮癌における癌幹細胞の同定とその免疫学的解析. (2009)
  • Author(s): 近松一朗, 岡本篤司, 初鹿恭介, 高橋吾郎, 増山敬祐.
  • Organizer: 第27回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 千葉
• [Presentation] Immunological properties of CD44+ cancer stem cells in squamous cell carcinoma of the head and neck. (2009)
  • Author(s): Chikamatsu K, et al.
  • Organizer: 2^<nd> World Congress of the International Academy of Oral Oncology
  • Place of Presentation: Toronto, Canada
• [Presentation] Cancer stem cells as mediators of treatment resistance in squamous cell carcinoma of the head and neck. (2009)
  • Author(s): Okamoto A, et al.
  • Organizer: 2^<nd> World Congress of the International Academy of Oral Oncology
  • Place of Presentation: Toronto, Canada
• [Presentation] Expansion and characterization of CD44+ cancer stem-like cells in squamous cell carcinoma of the head and neck. (2009)
  • Author(s): Chikamatsu K, et al.
  • Organizer: 100^<th> Annual Meeting American Association for Cancer Research
  • Place of Presentation: Denver, USA
• [Presentation] 頭頚部扁平上皮癌における癌幹細胞の同定とその免疫学的解析. (2009)
  • Author(s): 近松一朗, 他
  • Organizer: 第27回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 千葉
• [Presentation] Isolation and characterization of cancer stem cells in squamous cell carcinoma of the head and neck. (2008)
  • Author(s): 岡本篤司, 近松一朗, 坂倉浩一, 増山敬祐.
  • Organizer: 7^<th> International Conference on Head and Neck Cancer.
  • Place of Presentation: San Francisco, USA
• [Presentation] Thl/Th2 CD4+ T helper responses against wild-type sequence p53 peptides in HLA-DP5 patients with head and neck cancer. (2008)
  • Author(s): Chikamatsu K, Sakakura K, Okamoto A, Whiteside TL, DeLeo AB, Masuyama K
  • Organizer: 7^<th> International Conference on Head and Neck Cancer.
  • Place of Presentation: San Francisco, USA
• [Presentation] CD4+ Th responses to HLA-DP5-restricted wild-type sequence p53 peptides in patients with head and neck cancer. (2008)
  • Author(s): Chikamatsu K, Sakakura K, Yamamoto T, Whiteside TL, DeLeo AB, Masuyama K
  • Organizer: 99^<th> Annual Meeting American Association for Cancer Research.
  • Place of Presentation: San Diego, USA
• [Presentation] Isolation and characterization of cancer stem cells in squamous cell carcinoma of the head and neck. (2008)
  • Author(s): Okamoto A, et al.
  • Organizer: 7^<th> International Conference on Head and Neck Cancer
  • Place of Presentation: San Francisco
• [Presentation] Th1/Th2 CD4+ T helper responses against wild-type sequence p53 peptides in HLA-DP5 patients with head and neck cancer (2008)
  • Author(s): Chikamatsu K, et al.
  • Organizer: 7^<th> International Conference on Head and Neck Cancer
  • Place of Presentation: San Francisco
• [Presentation] CD4+ Th responses to HLA-DP5-restricted wild-type sequence p53 peptides in patients with head and neck cancer. (2008)
  • Author(s): Ch ikamatsu K, et al.
  • Organizer: 99^<th> Annual Meeting American Association for Cancer Research
  • Place of Presentation: San Diego