Identification of human corneal limbal stem cell markers based on the method of epithelial cell-lineaged stem cell isolation

• Project/Area Number: 20592072
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: YAMAGAMI Satoru Tokyo Women's Medical University, 医学部, 准教授 (10220245)
• Co-Investigator(Kenkyū-buntansha): OKOO Seiichi 東京大学, 医学部, 特任研究員 (20345052)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 角膜上皮細胞 / 幹細胞 / ラミニン5 / 結膜上皮細胞 / 杯細胞 / 角膜輪部上皮細胞 / サイトケラチン / 角膜上皮 / 結膜上皮 / bFGF

Research Abstract

We tried to isolate human conjunctival epithelial progenitor cells using laminin-5 expressing highly adherent cells isolation method. As a result, we have isolated conjunctival epithelial progenitor cells, which could differentiate into epithelial cells and goblet cells. Our data suggest that we can identify isolation marker by comparing character between conjunctival and corneal limbal stem cells.

Research Products

• [Journal Article] Adhesion, migration, and proliferation of cultured human corneal endothelial cells by laminin-5. (2011)
  • Author(s): Yamaguchi M, Ebihara N, Shima N, Kimoto M, Funaki T, Yokoo S, Murakami A, Yamagami S
  • Journal Title: Investigative Ophthalmology & Visual Science 52 Pages: 679-84
  • Peer Reviewed
• [Journal Article] Adhesion, migration, and proliferation of cultured human corneal endothelial cells by laminin-5 (2011)
  • Author(s): Yamaguchi M, Ebihara N, Shima N, Kimoto M, Funaki T, Yokoo S, Murakami A, Yamagami S
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 52 Pages: 679-684
  • Peer Reviewed
• [Journal Article] 羊膜を使った第角膜再生 (2010)
  • Author(s): 山上聡
  • Journal Title: 治療 92 Pages: 176-180
• [Journal Article] Isolation of adult progenitor cells with neuronal potential from rabbit corneal epithelial cells in serum-and feeder layer-free culture conditions. (2010)
  • Author(s): Mimura T, Yamagami S, Uchida S, Yokoo S, Ono K, Usui T, Amano S
  • Journal Title: Molecular Vision 24;16 Pages: 1712-1719
  • Peer Reviewed
• [Journal Article] Selective isolation of young cells from human corneal endothelium by the sphere-forming assay. (2010)
  • Author(s): Mimura T, Yamagami S, Yokoo S, Usui T, Amano S
  • Journal Title: Tissue Engeering Part C Methods. 16 Pages: 803-812
  • Peer Reviewed
• [Journal Article] Isolation of adult progenitor cells with neuronal potential from rabbit corneal epithelial cells in serum- and feeder layer-free culture conditions. (2010)
  • Author(s): Mimura T, Yamagami S, Uchida S, Yokoo S, Ono K, Usui T, Amano S
  • Journal Title: Molecular Vision Volume: 24 Pages: 1712-1719
  • Peer Reviewed
• [Journal Article] Selective isolation of young cells from human corneal endothelium by the sphere-forming assay. (2010)
  • Author(s): Mimura T, Yamagami S, Yokoo S, Usui T, Amano S
  • Journal Title: Tissue Engineering Part C Methods Volume: 16 Pages: 803-812
  • Peer Reviewed
• [Journal Article] 羊膜を使った再生医療 (2010)
  • Author(s): 山上聡
  • Journal Title: 治療 92 Pages: 176-180
• [Journal Article] スティーブンス・ジョンソン症候群 (2009)
  • Author(s): 山上聡
  • Journal Title: 全身疾患と眼所見Current Therapy 27 Pages: 50-52
• [Journal Article] Human corneal endothelial cells expressing programmed death-ligand 1(PD-L1) suppress PD-1^+ T helper 1 cells by a contact-dependent mechanism. (2009)
  • Author(s): Sugita S, Usui Y, Horie S, Futagami Y, Yamada Y, Ma J, Kezuka T, Hamada H, Usui T, Mochizuki M, Yamagami S
  • Journal Title: Investigative Ophthalmology & Visual Science 50 Pages: 263-272
  • Peer Reviewed
• [Journal Article] Immunologic mechanisms of corneal allografts reconstituted from cultured allogeneic endothelial cells in an immune-privileged site. (2009)
  • Author(s): Hayashi T, Yamagami S, Tanaka K, Yokoo S, Usui T, Amano S, Mizuki N
  • Journal Title: Investigative Ophthalmology & Visual Science 50 Pages: 3151-3158
  • Peer Reviewed
• [Journal Article] ステイーブンス・ジョンソン症候群 (2009)
  • Author(s): 山上聡
  • Journal Title: Current Therapy 27 Pages: 888-890
• [Journal Article] 拒絶反応の無い理想的な角膜移植手術を目指して-全層角膜移植から内皮細胞移植へ- (2008)
  • Author(s): 山上聡
  • Journal Title: 日本眼科学会雑誌 112 Pages: 266-278
  • Peer Reviewed
• [Journal Article] Isolation and distribution of rabbit keratocyte precursors. (2008)
  • Author(s): Mimura T, Amano S, Yokoo S, Uchida S, Usui T, Yamagami S
  • Journal Title: Molecular Vision 14 Pages: 197-203
  • Peer Reviewed
• [Journal Article] A mouse model of allogeneic corneal endothelial cell transplantation. (2008)
  • Author(s): Hayashi T, Yamagami S, Tanaka K, Yokoo S, Usui T, Amano S, Mizuki N
  • Journal Title: Cornea 27 Pages: 699-705
  • Peer Reviewed
• [Journal Article] Human corneal epithelial equivalents for ocular surface reconstruction in complete serum free culture system without unknown factors. (2008)
  • Author(s): Yokoo S, Yamagami S, Usui T, Amano S, Araie M
  • Journal Title: Investigative Ophthalmology & Visual Science 49 Pages: 2438-2443
  • Peer Reviewed
• [Journal Article] Anovel isolation technique of progenitor cells in human corneal epithelium using non-tissue culture dishes. (2008)
  • Author(s): Yokoo S, Yamagami S, Shimada T, Usui T,Sato T, Amano S, Araie M, Hamuro J
  • Journal Title: Stem Cells 26 Pages: 1743-1748
  • Peer Reviewed
• [Journal Article] A novel isolation technique of progenitor cells in human corneal epithelium using non-tissue culture dishes. (2008)
  • Author(s): Seiichi Yokoo, Satoru Yamagami, et al.
  • Journal Title: Stem Cells 26 Pages: 1743-1748
  • Peer Reviewed
• [Journal Article] Human corneal epithelial equivalents for ocular surface reconstruction in a complete serum-free culture system without unknown factors. (2008)
  • Author(s): Seiichi Yokoo, Satoru Yamagami, et al.
  • Journal Title: Invest Ophthalmol Vis Sci 49 Pages: 2438-2443
  • Peer Reviewed
• [Presentation] Fibroblast growth factor 2 promotes differentiation of human keratinocyte progenitor cells to goblet cells. (2010)
  • Author(s): 山上聡, 横尾誠一
  • Organizer: ARVO (The Association for Research in Vision and Ophthalmology)
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2010-05-03
• [Presentation] 浮遊細胞用培養皿を用いたヒト第角膜輪部上皮前駆細胞の新規単離法 (2010)
  • Author(s): 横尾誠一
  • Organizer: 第114回日本眼科学会総会 日本眼科学会学術奨励賞記念講演
  • Year and Date: 2010-04-17
• [Presentation] EGF刺激による角膜輪部上皮前駆細胞の細胞内シグナル伝達経路 (2010)
  • Author(s): 横尾誠一、山上聡、天野史郎、新家眞
  • Organizer: 第114回日本眼科学会総会
  • Year and Date: 2010-04-15
• [Presentation] KGF添加無フィーダー細胞・無血清角膜上皮細胞シートのサイトケラチン15の発現 (2010)
  • Author(s): 横尾誠一、山上聡、天野史郎、新家眞
  • Organizer: 第34回角膜カンファランス・第26回日本角膜移植学会
  • Year and Date: 2010-02-11
• [Presentation] 角膜内皮細胞の免疫学的特徴と再生医療への期待 (2009)
  • Author(s): 山上聡
  • Organizer: 第63回日本臨床眼科学会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-10-09
• [Presentation] ヒト角膜輪部上皮前駆細胞の分化能の検討 (2009)
  • Author(s): 横尾誠一、臼井智彦、三村達哉、杉崎顕史、天野史郎、山上聡
  • Organizer: 第113回日本眼科学会総会
  • Year and Date: 2009-04-17
• [Presentation] ヒト角膜輪部上皮前駆細胞の分化能の検討 (2009)
  • Author(s): 横尾誠一
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-17
• [Presentation] Anovel isolation technique of limbal epithelial progenitor cells using uncoated dishes. (2008)
  • Author(s): Yamagami S, Yokoo S , Usui T, Amano S
  • Organizer: ARVO (The Association for Research in Vision and Ophthalmology)
  • Place of Presentation: Fort Lauderdale, USA
  • Year and Date: 2008-05-01
• [Presentation] A novel isolation technique of limbal epithelial progenitor cells using uncoated dishes (2008)
  • Author(s): Satoru Yamagami
  • Organizer: The association for research in vision and ophthal mology
  • Place of Presentation: Fortlauderdale, in USA
  • Year and Date: 2008-05-01
• [Presentation] 眼科幹細胞研究の現状.角膜の幹細胞研究 (2008)
  • Author(s): 山上聡
  • Organizer: 第112回日本眼科学会総会シンポジウム
  • Place of Presentation: 横浜市
  • Year and Date: 2008-04-17
• [Presentation] ヒト角結膜の組織幹細胞・前駆細胞の特徴シンポジウム 眼科幹細胞研究の現状 (2008)
  • Author(s): 横尾誠一
  • Organizer: 第112回日本眼科学会総会
  • Year and Date: 2008-04-17
• [Book] 細胞分離・操作技術の最前線(ヒト第角膜の前駆細胞・組織幹細胞の分離) (2008)
  • Author(s): 横尾誠一、山上聡