Corneal Tissue Engineering Using Alginate

• Project/Area Number: 20592083
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Doshisha University
• Principal Investigator: KOIZUMI Noriko Doshisha University, 生命医科学部, 教授 (20373087)
• Co-Investigator(Kenkyū-buntansha): KINOSHITA Shigeru 京都府立医科大学, 医学研究科, 教授 (30116024)
• Research Collaborator: TORII Ryuzou 滋賀医科大学, 動物生命科学研究センター, 教授 (50106647) ; OKUMURA Naoki 京都府立医科大学, 眼科, 病院助教 (10581499)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 角膜再生医療 / アルジネート / 細胞移植 / 角膜 / 再生医療 / 組織保存

Research Abstract

We tried to develop a new cryopreservation method for human corneal tissue using alginate encapsulation and we studied the feasibility of cultivated corneal endothelial cell transplantation using a corneal lamellar graft. We cultivated monkey corneal endothelial cells on a frozen preserved human corneal lamellar graft and successfully transplanted this construct into animal eyes. Our results suggest the possibility of cultivated corneal endothelial cell transplantation using a frozen preserved corneal lamellar graft.

Research Products

• [Journal Article] 臨床応用を目指した角膜内皮再生医療の開発 (2010)
  • Author(s): 小泉範子
  • Journal Title: 同志社大学生命医科学会報 第2号 Pages: 2-7
  • NAID: 110008792409
• [Journal Article] 角膜疾患の未来医療 (2010)
  • Author(s): 木下茂、小泉範子, 他
  • Journal Title: 日本眼科学会雑誌 Volume: 114 Pages: 161-199
  • NAID: 10026261146
  • Peer Reviewed
• [Journal Article] Enhancement on primate corneal endothelial cell survival in vitro by a ROCK inhibitor (2009)
  • Author(s): Okumura N, Koizumi N, Kinoshita S, et al
  • Journal Title: Invest Ophthalmol Vis Sci. 50(8) Pages: 3680-3687
  • Peer Reviewed
• [Journal Article] Cultivated Corneal Endothelial Transplantation in a Primate : Possible Future Clinical Application in Corneal Endothelial Regenerative Medicine. (2008)
  • Author(s): Koizumi N, Kinoshita S, et al.
  • Journal Title: Cornea. 27(8)
  • Peer Reviewed
• [Presentation] New strategy for corneal endothelial tissue engineering. (2011)
  • Author(s): 小泉範子
  • Organizer: Asia-ARVO 2011
  • Place of Presentation: Singapore
  • Year and Date: 2011-01-20
• [Presentation] New strategy for corneal endothelial tissue engineering (2010)
  • Author(s): Koizumi N
  • Organizer: 2^<nd> Asia Cornea Society Biennial Scientific Meeting
  • Place of Presentation: The Westin Miyako, Kyoto, Japan
  • Year and Date: 2010-11-30
• [Presentation] カニクイザルを用いた角膜内皮再生医療の開発 (2010)
  • Author(s): 小泉範子
  • Organizer: 第14回予防衛生協会セミナー
  • Place of Presentation: つくば(平成21年度予防衛生協会研究奨励賞受賞講演)
  • Year and Date: 2010-11-19
• [Presentation] The existence of dead cells in corneal endothelium preserved with storage media. (2010)
  • Author(s): Tanioka H, Kawasaki S, Adachi H, Inatomi T, Hieda O, Fukuoka H, Okumura N, Koizumi N, Iliakis B, Kinoshita S
  • Organizer: Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO)
  • Place of Presentation: Fort Lauderdale, Florida, USA
  • Year and Date: 2010-05-04
• [Presentation] The efficacy of a ROCK inhibitor containing corneal storage medium for corneal endothelial cells. (2010)
  • Author(s): Takahashi H, Koizumi N, Okumura N, Ueno M, Sakamoto Y, Hamuro J, Kinoshita S
  • Organizer: Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO)
  • Place of Presentation: Fort Lauderdale, Florida, USA
  • Year and Date: 2010-05-04
• [Presentation] Cultivated corneal endothelial transplantation in a primate for the future clinical application of corneal endothelial regenerative medicine (2010)
  • Author(s): 小泉範子
  • Organizer: Gordon Research Conference Biology & Pathobiology of the Cornea
  • Place of Presentation: Ventura, USA
  • Year and Date: 2010-03-11
• [Presentation] Cultivated corneal endothelial transplantation in a primate for the future clinical application of corneal endothelial regenerative medicine. (2009)
  • Author(s): Koizumi N
  • Organizer: the 107th Congress of the German Society of Ophthalmology
  • Place of Presentation: Congress Center Leipzig(ドイツ)
  • Year and Date: 2009-09-24
• [Presentation] 角膜内皮再生医療の現状と展望 (2009)
  • Author(s): 小泉範子
  • Organizer: 第115回京都眼科学会
  • Place of Presentation: 京都大学芝蘭会館(京都)
  • Year and Date: 2009-09-06
• [Presentation] Cultivated monkey corneal endothelial cell transplantation using corneal lamellar graft. (2009)
  • Author(s): N.Koizumi, et al.
  • Organizer: 2009 Annual Meeting of the Association for Research in Vision and Ophthalmology, Fort Lauderdale
  • Place of Presentation: Florida, USA
  • Year and Date: 2009-05-04
• [Presentation] Cultivated Monkey Corneal Endothelial Cell Transplantation Using Corneal Lamellar Graft. (2009)
  • Author(s): Koizumi, N., et al.
  • Organizer: 2009 Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Greater Fort Lauderdale/Broward County Convention Center(米国)
  • Year and Date: 2009-05-04
• [Presentation] 霊長類を用いた培養角膜内皮移植術の開発 (2009)
  • Author(s): 小泉範子
  • Organizer: 第113回日本眼科学会総会
  • Place of Presentation: 東京(日本眼科学会学術奨励賞記念講演)
  • Year and Date: 2009-04-18
• [Presentation] 霊長類を用いた培養角膜内皮移植術の開発 (2009)
  • Author(s): 小泉範子, 他
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-05
• [Presentation] ヒト角膜実質を用いた培養角膜内皮移植術の試み (2009)
  • Author(s): 小泉範子, 他
  • Organizer: 第33回角膜カンファランス・第25回角膜移植学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-02-21
• [Presentation] Cultivated corneal endothelial cell sheet transplantation in a primate mode. (2008)
  • Author(s): 小泉範子, et al
  • Organizer: XVIII International Congress of Eye Research (ICER)
  • Place of Presentation: Beijing, China
  • Year and Date: 2008-09-25
• [Presentation] Cryopreservation of corneal tissue combined with alginate encapsulation. (2008)
  • Author(s): Connon CJ, Koizumi N, et al.
  • Organizer: 2008 Annual Meeting of the Association for Research in Vision and Ophthalmology, Fort Lauderdale
  • Place of Presentation: Florida, U.S.A.
  • Year and Date: 2008-04-28
• [Presentation] Cryopreservation of Corneal Tissue Combined With Alginate Encapsulation. (2008)
  • Author(s): Connon CJ (Koizumi N)
  • Organizer: 2008 Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Fort Lauderdale, Florida, U. S. A
  • Year and Date: 2008-04-28
• [Book] encyclopedia of the eye.(The Ocular Surface : The surgical treatment for corneal epithelial stem cell deficiency, corneal epithelial defect, and peripheral corneal ulcer.) (2011)
  • Author(s): Koizumi N, Kinoshita S
  • Publisher: Elsevier Ltd., Oxfordshire(in press)