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Implication for long-term hypothermia of degradation of cytokine mRNA in endothelial cells stimulated with lipopolysaccharides

Research Project

Project/Area Number 20592129
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Emergency medicine
Research InstitutionNihon University

Principal Investigator

SAKURAI Atsushi  Nihon University, 医学部, 助教 (40339320)

Co-Investigator(Kenkyū-buntansha) KINOSHITA Kosaku  日本大学, 医学部, 准教授 (90260968)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords内皮細胞 / 長期低温 / 炎症 / LPS / IL-8 / mRNA安定性 / 低温 / サイトカイン / 人臍帯血内皮細胞 / interleukin (IL)-8 / interleukin (IL)-6 / mRNAの安定性
Research Abstract

This experimental study investigated the production of interleukin (IL)-8 protein and its mRNA expression and stability in endothelial cells stimulated by lipopolysaccharides (LPS) and undergoing long-term hypothermia. Under long-term hypothermia, IL-8 protein production was suppressed, while IL-8 mRNA was stabilized at the point of turn-over and expression increased. The potential of IL-8 production for the inflammatory stimulation in endothelial cells may persist during long-term hypothermia.

Report

(5 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Products Report
  • Research Products

    (9 results)

All 2012 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (7 results)

  • [Journal Article] Implication for Long-Term Hypothermia on Degradation of Interleukin-8 mRNA in Endothelial Cells Stimulated with Lipopolysaccharides2012

    • Author(s)
      Atsushi Sakurai
    • Journal Title

      Therapeutic Hypothermia and Temperature Management

      Volume: 2 Issue: 2 Pages: 67-72

    • DOI

      10.1089/ther.2012.0010

    • Related Report
      Products Report
    • Peer Reviewed
  • [Journal Article] Changes caused by hypothermia in interleukin-8 mRNA stability in human endothelial cells stimulated by lipopolysaccharide2010

    • Author(s)
      Atsushi Sakurai, Kosaku Kinoshita, Rikimaru Kogawa, Tsukasa Kuwana, Takashi Moriya, Akihiro Noda, Katsuhisa Tanjoh
    • Journal Title

      Critical Care Medicine 37巻12号(suppl)

    • Related Report
      2010 Final Research Report
  • [Presentation] Changes caused by hypothermia in interleukin-8 mRNA stability in human endothelial cells stimulated by lipopolysaccharide.2010

    • Author(s)
      櫻井淳
    • Organizer
      Society of critical care medicine 39^<th> critical care congress 2010
    • Place of Presentation
      Miami (USA)
    • Year and Date
      2010-01-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Changes caused by hypothermia in interleukin-8 mRNA stability in human endothelial cells stimulated by lipopolysaccharide2010

    • Author(s)
      Atsushi Sakurai
    • Organizer
      Society of critical care medicine 39^<th> critical care congress
    • Place of Presentation
      Miami, USA
    • Year and Date
      2010-01-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Lipopolysaccharide刺激で長期低温培養した内皮細胞でのinterleukin-8の蛋白産生とmRNA発現の経時的変化2009

    • Author(s)
      櫻井淳
    • Organizer
      第15回日本エンドトキシン研究会
    • Place of Presentation
      東京
    • Year and Date
      2009-11-14
    • Related Report
      2010 Final Research Report
  • [Presentation] Lipopolysaccharide刺激下に低温培養した人臍帯血内皮細胞でのActinomicin DによるIL-6とIL-8のmRNAの安定性の検討2009

    • Author(s)
      櫻井淳
    • Organizer
      第37回日本救急医学会
    • Place of Presentation
      盛岡
    • Year and Date
      2009-10-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Lipopolysaccharide刺激下に培養した人臍帯血内皮細胞での低温によるIL-6とIL-8のmRNAの安定性変化の検討2009

    • Author(s)
      櫻井淳
    • Organizer
      第13回日本脳低温療法学会
    • Place of Presentation
      札幌
    • Year and Date
      2009-07-03
    • Related Report
      2010 Final Research Report
  • [Presentation] 長期低温下培養にlipopolysaccharideで刺激した人臍帯血内皮細胞におけるinterleukin-8の蛋白産生とmRNA発現の経時的変化2008

    • Author(s)
      櫻井淳
    • Organizer
      第36回日本救急医学会
    • Place of Presentation
      札幌
    • Year and Date
      2008-10-14
    • Related Report
      2010 Final Research Report
  • [Presentation] Lipopolysaccharide刺激下に長期低体培養した人臍帯血内皮細胞でのinterleukin-8の蛋白産生とmRNA発現の経時的変化2008

    • Author(s)
      櫻井淳
    • Organizer
      第11回日本脳低温療法学会
    • Place of Presentation
      岐阜
    • Year and Date
      2008-07-04
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-09-09  

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