Implication for long-term hypothermia of degradation of cytokine mRNA in endothelial cells stimulated with lipopolysaccharides
| Project/Area Number |
20592129
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Nihon University |
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Principal Investigator |
SAKURAI Atsushi Nihon University, 医学部, 助教 (40339320)
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| Co-Investigator(Kenkyū-buntansha) |
KINOSHITA Kosaku 日本大学, 医学部, 准教授 (90260968)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 内皮細胞 / 長期低温 / 炎症 / LPS / IL-8 / mRNA安定性 / 低温 / サイトカイン / 人臍帯血内皮細胞 / interleukin (IL)-8 / interleukin (IL)-6 / mRNAの安定性 |
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| Research Abstract |
This experimental study investigated the production of interleukin (IL)-8 protein and its mRNA expression and stability in endothelial cells stimulated by lipopolysaccharides (LPS) and undergoing long-term hypothermia. Under long-term hypothermia, IL-8 protein production was suppressed, while IL-8 mRNA was stabilized at the point of turn-over and expression increased. The potential of IL-8 production for the inflammatory stimulation in endothelial cells may persist during long-term hypothermia.
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Report
(5 results)
Research Products
(9 results)
