Are odontogenesis-related genes detected with microarray really involved in odontogenesis?
| Project/Area Number |
20592151
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Morphological basic dentistry
|
|---|
| Research Institution | Tokyo Dental College |
|---|
Principal Investigator |
MURAMATSU Takashi Tokyo Dental College, 歯学部, 講師 (00276982)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Hitoshi 日本大学, 松戸歯学部, 講師 (80265165)
KOHNO Yohko 昭和大学, 歯学部, 准教授 (40195681)
SHIMONO Masaki 東京歯科大学, 歯学部, 教授 (00085771)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 歯牙形成性能 / 歯胚 / 歯乳頭 / 遺伝子導入 / 歯牙再生 / 歯牙形成 / Adamts4 / Aldh1a2 / Lef-1 / 遺伝子解析 / in situ hybridization / Aldhla2 |
|---|
| Research Abstract |
Important factors involved in odontogenesis in mouse dental papillae disappear between the pre- and post-natal stages of development. Therefore, we hypothesized that certain genes involved in odontogenesis in dental papillae were subject to pre-/post-natal down-regulation. Our goal was to identify, by microarray analysis, which genes were down-regulated. Dental papillae were isolated from embryonic 16-day-, 18-day- (E16, E18), and post-natal 3-day-old (P3) murine first mandibular molar germs and analyzed by microarray. The number of down-regulated genes was 2269 between E16 and E18, and 3130 between E18 and P3. Drastic down-regulation (fold change > 10.0) of Adamts4, Aldha1a2, and Lef1 was observed at both E16 and E18, and quantitative RT-PCR revealed a post-natal reduction in their expression (Adamts4, 1/3; Aldh1a2, 1/13; and Lef1, 1/37). These results suggest that down-regulation of these three genes is an important factor in normal odontogenesis in dental papillae. To confirm whether these genes are involved in odontogenesis or not, we constructed expression vectors of these genes. We transfected the genes into adult pulp cells and combined with dental epithelium, and transplanted under kidney capsule. However, odontogenesis could not be seen histologically. Further investigation is necessary to confirm that these genes are involved in odontogenesis.
|
Report
(4 results)
Research Products
(58 results)
