Analysis of the elevated expression of cartducin gene in osteosarcoma and the mechanisms of tumor development

• Project/Area Number: 20592197
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Osaka University
• Principal Investigator: MAEDA Takashi Osaka University, 大学院・歯学研究科, 講師 (80324789)
• Co-Investigator(Kenkyū-buntansha): FURUKAWA SOUHEI 大阪大学, 大学院・歯学研究科, 教授 (80173524)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: カートデューシン / 骨肉腫細胞 / 増殖 / 遊走 / 浸潤 / ERK1 / 2 / ERK1/2 / p38-MAPK / TGF-beta

Research Abstract

Cartducin is expressed in human osteosarcomas. The mRNA level of Cartducin was increased in murine osteosarcoma cell lines compared with its level in normal murine osteoblastic cells. The protein level of Cartducin was also increased in these osteosarcoma cell lines. Stimulation of these osteosarcoma cells by Cartducin promoted tumor cell growth but not migration in vitro. Further, Cartducin stimulation led to the activation of extracellular signal-regulated kinase 1/2 (ERK1/2) in these osteosarcoma cell lines. Our results suggest that Cartducin expression may play a role in osteosarcoma tumor growth associated with activation of the ERK1/2 signaling pathway.

Research Products

• [Journal Article] Functional analysis of CTRP3/cartducin to Meckel's cartilage and developing condylar cartilage in fetal mouse mandible. (2011)
  • Author(s): Yokohama-Tamaki T, Maeda T, Shibata S.
  • Journal Title: J.Anat. 218 Pages: 517-533
  • Peer Reviewed
• [Journal Article] CTRP3/cartducin is induced by transforming growth factor-beta1 and promotes vascular smooth muscle cell proliferation. (2010)
  • Author(s): Maeda T, Wakisaka S.
  • Journal Title: Cell Biol.Int. 34 Pages: 261-266
  • Peer Reviewed
• [Journal Article] CTRP3/cartducin is induced by transforming growth factor-betaland promotes vascular smooth muscle cell proliferation (2010)
  • Author(s): Maeda T, Wakisaka S
  • Journal Title: Cell Biology International Volume: 34 Pages: 261-266
  • Peer Reviewed
• [Journal Article] Elevated expression of CTRP3/cartducin contributes to promotion of osteosarcoma cell proliferation (2009)
  • Author(s): Akiyama H, Furukawa S, Wakisaka S, Maeda T.
  • Journal Title: Oncol.Rep. 21 Pages: 1477-1481
  • Peer Reviewed
• [Journal Article] Assessment of influence of smoking, drinking, leukoplakia and dental irritation on local control of early oral tongue carcinoma treated with brachytherapy : age and dental factors are potential prognostic factors. (2009)
  • Author(s): Yamazaki H, Inoue T, Yoshida K, Kotsuma T, Yoshioka Y, koizumi M, Furukawa S, Kakimoto N, Shimizutani K, Nishimura T.
  • Journal Title: Tumori 95 Pages: 461-466
  • Peer Reviewed
• [Journal Article] Diagnostic imaging findings for mandibular metastasis from gastric adenocarcinoma. (2009)
  • Author(s): Uchiyama Y, Murakami S, Kakimoto N, Nakatani A, Kishino M, Hamab Y, Furukawa S.
  • Journal Title: Oral Surg.Oral Med.Oral Pathol.Oral Radiol.Endod. 107 Pages: 49-53
  • Peer Reviewed
• [Journal Article] Metallic artifacts caused by dental metal prostheses on caused by dental metal prostheses on PET images : a PET/CT phantom study using different PET/CT scanners. (2009)
  • Author(s): Shimamoto H, Kakimoto N, Fujino K, Hamada S, Shimosegawa E, Murakami S, Furukawa S, Hatakezawa J.
  • Journal Title: Ann.Nucl.Med. 23 Pages: 443-449
  • Peer Reviewed
• [Journal Article] Elevated expression of CTRP3/cartducin contributes to promotion of osteosarcoma cell proliferation (2009)
  • Author(s): Akiyama H, Furukawa S, Wakisaka S, Maeda T
  • Journal Title: Oncology Reports Volume: 21 Pages: 1477-1481
  • Peer Reviewed
• [Journal Article] Retinoic acid affects craniofacial patterning by changing Fgf8 expression in the pharyngeal ectoderm. (2008)
  • Author(s): Abe M, Maeda T, Wakisaka S.
  • Journal Title: Dev.Growth Differ. 50 Pages: 717-729
  • NAID: 10023963261
  • Peer Reviewed
• [Journal Article] (18)F-FDG accumulation in the oral cavity is associated with periodontal disease and apical periodontitis : an initial demonstraion on PET/CT. (2008)
  • Author(s): Shimamoto S, Tatsumi M, Kakimoto N, Hamada S, Shimosegawa E, Murakami S, Furukawa S, Hatakezawa J.
  • Journal Title: Ann.Nucl.Med. 22 Pages: 587-593
  • Peer Reviewed
• [Journal Article] Retinoic acid affects craniofacial patterning by changing Fgf8 expression in the pharyngeal ectoderm (2008)
  • Author(s): Abe M, Maeda T, Wakisaka S
  • Journal Title: Development, Growth & Differentiation 50 Pages: 717-729
  • NAID: 10023963261
  • Peer Reviewed
• [Presentation] 骨肉腫細胞におけるC1qTNFファミリー分泌蛋白CTRP3/cartducinの発現増強と増殖促進作用 (2009)
  • Author(s): 前田隆史、脇坂聡
  • Organizer: 第51回歯科基礎医学会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-11
• [Presentation] 骨肉腫細胞におけるC1qTNFファミリー分泌蛋白CTRP3/cartducinの発現増強と増殖促進作用 (2009)
  • Author(s): 前田隆史
  • Organizer: 第51回 歯科基礎医学会
  • Place of Presentation: 朱鷺メッセ新潟コンベンションセンター(新潟)
  • Year and Date: 2009-09-11
• [Presentation] Function of cartducin in mandibular condylar cartilage formation (2009)
  • Author(s): Yokohama-Tamaki T, Maeda T, Shibata S
  • Organizer: 2nd Meeting of International Association for Dental Research (IADR) Pan Asian Pacific Federation (PAPF)
  • Place of Presentation: Wuhan, China
• [Presentation] マウス下顎頭軟骨の発生過程におけるCartducinの発現と機能解析 (2008)
  • Author(s): 田巻玉器、前田隆史,柴田俊一
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-24
• [Remarks] ホームページ等
  • URL: http://www.osaka-u.ac.jp/ja/research/list.html