| Project/Area Number |
20592271
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Prosthetic dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KASHIWABARA Toshiya The University of Tokushima, 病院, 助教 (90274232)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIKAWA Tetsuo 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (90193432)
HONGAMA Seiko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (60380078)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAKE Yoichiro 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (80136093)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | エリスリトール / 糖アルコール / カンジダ / 義歯洗浄剤 / カンジダアルビカンス / バイオフィルム / 機能水 |
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| Research Abstract |
The purpose of the present study was to develop a new and safer denture cleanser with "Erythritol", one of sugar alcohols for preventing dental caries, periodontal disease, stomatitis and aspiration pneumonia through dental and denture plaque control.
In vitro experiment, candidal biofilm formation and assessment of sterilized effect were established. An in vitro Candida albicans biofilm was formed on a plastic coverslip coated with type I collagen following two methods and were compared : 1) yeast nitrogen base (YNB) /100 mM glucose medium supplemented with 2.5 mM N-acetylglucosamine (GlcNAc) ; and 2) BHI medium. The former method was employed in this experiment. The quantitative CFU counting in SEM images was employed as the assessment of sterilized effect. The enhancement of the fungicidal effect was evaluated using microbial assay after treating the biofilm with the test solutions (sugar alcohols : erythritol, xylitol, and sorbitol, each containing BTC solution). No fungicidal effect was observed with sugar alcohols without BTC. The
fungicidal effect of erythritol-containing BTC increased with the erythritol concentration. Further, the level of enhancement of erythritol was the highest in three sugar alcohols. Sugar alcohols, especially erythritol, enhanced the fungicidal effect of sterilized agents toward in vitro candidal biofilms. In vivo experiment, the sterilized effect of the solution with and without erythritol on the sterilization of periodontal pocket was evaluated by Randomized Control Trials and PCR test. The sterilized effect of the solution with erythritol was somewhat higher.
The sterilized solution with sugar alcohols, especially erythritol would be a safe and effective denture cleanser.
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