Functional analysis of cholesterol synthetic pathway in osteochondrodysplasia of congenital cranio-maxillo-facial anomalies
| Project/Area Number |
20592336
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
SUMI Ken-saku Hiroshima University, 大学院・医歯薬学総合研究科, 助教 (30403629)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUI Yasuto 広島大学, 大学院・医歯薬学総合研究科, 助教 (90363085)
OKAMOTO Tetsuji 広島大学, 大学院・医歯薬学総合研究科, 教授 (00169153)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 軟骨分化 / 骨分化 / コレステロール合成 / ステロール代謝 / ATDC5細胞 / MC3T3細胞 / 細胞増殖因子 / sonic hedgehog / 頭蓋・顎・顔面 / 骨・軟骨異常 / 唇顎口蓋裂 / メタボローム解析 / ソニックヘッジホッグ / 先天異常 |
|---|
| Research Abstract |
Purpose : In this study, we have studied the effect of C27 and C30 sterols on growth and differentiation of chondrogenic cell line, ATDC5 cells in serum-free cell culture.
Material and Methods : Mouse chondrogenic cell line ATCD5 cells have been used to study the effect of cholesterol and its precursor (C27 sterol and C30 sterol) on the growth and differentiation at early and late stage. The expression of the C14-demethylase, 3-ketoreductase, C4-demethylase, and several marker genes in the process of chondrogenic differentiation of the cells have been studied by RT-PCR analysis.
Results and discussion : By using ATDC5 cell line, which mimic in vivo chondrogenic differentiation process, we have studied effect of C27 and C30 sterols on the growth and differentiation of the cells. As a result, we have found that exogenously added C27 sterols has various effect on the growth and differentiation of the cell, suggesting that C27 sterol has an important role in the growth and differentiation of the ATDC5 cell line.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] 当科における口腔顎顔面骨折の臨床統計的検討2011
Author(s)
坂上泰士, 福井康人, 伊藤翼, 山崎佐知子, 岡崎文彦, 木村直大, 竹末奈七子, 鍋島巧, 石田康隆, 浜名智昭, 角健作, 岡本康正, 神田拓, 新谷智章, 吉岡幸男, 谷亮治, 林堂安貴, 虎谷茂昭, 岡本哲治
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Peer Reviewed
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[Journal Article] FGFR1 abrogates inhibitory effect of androgen receptor concurrent with induction of androgen-receptor variants in androgen receptor-negative prostate tumor epithelial cells.2011
Author(s)
Kobayashi M., Huang, Y., Jin, C., Luo, Y., Okamoto T., Wang, F., McKeehan, WL.
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Peer Reviewed
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[Journal Article] Coincident two mutations and one single nucleotide polymorphism of the PTCH1 gene in a family with naeboid basal cell carcinoma syndrome.2008
Author(s)
Abe, S., Kabashima, K., Sakabe, J., Shimauchi, T., Zhang, Y., Okamoto T., Tokura, Y.
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Journal Title
Acta Derm Venereol 88(6)
Pages: 635-636
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Peer Reviewed
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[Book] 口腔外科学2010
Author(s)
白砂兼光, 古郷幹彦, 他
Total Pages
815
Publisher
医歯薬出版
Related Report
