Molecular mechanism of cyto-differentiation of proliferating bone resorping lesions

• Project/Area Number: 20592349
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: KAWAKAMI Toshiyuki Matsumoto Dental University, 大学院・歯学独立研究科, 教授 (80104892)
• Co-Investigator(Kenkyū-buntansha): KEISUKE Nakano 松本歯科大学, 歯学部, 准教授 (10325095) ; NAGATSUKA Hitoshi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70237535)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 口腔外科学一般 / 歯原性腫瘍 / エナメル上皮腫 / 石灰化嚢胞性歯原性腫瘍 / 石灰化歯原性腫瘍 / 軟骨肉腫 / Notch / Jagged / Delta / ameloblastoma / Notch signaling / 細胞分化 / 細胞増殖 / 免疫組織化学 / 腫瘍の組織型 / 骨吸収 / 顎骨 / 腫瘍 / 歯 / シグナリング

Research Abstract

We examined Notch signaling molecules, Notch1 and Jagged1, in serial large cases of typical solid/multicystic ameloblastoma. In summary, the results showed that these morphogenesis regulation Notch and related factors are closely related to cytological differentiation in neoplastic cells of ameloblastoma. The Notch and Jagged positive-cell ratio was frequently positive, and was nearly the same between the varied histopathological, cytological patterns; however, the less-differentiated cells were slightly fewer in number that that of well-differentiated cells.

Research Products

• [Journal Article] An unsuspected ameloblastoma in the subpontic region of the mandible with consideration of pathogenesis from the radiographic course. (2010)
  • Author(s): Siar CH, Nakano K, Chelvanayagam PI, Ng KH, Nagatsuka H, Kawakami T
  • Journal Title: Eur J Med Res 15(Peerreviewed) Pages: 153-158
• [Journal Article] Squamous odontogenic tumor of the mandible : A case report demonstrating immunoexpression of Notch1, 3, 4, Jagged1, 2 and Deltal. (2010)
  • Author(s): Siar CH, Nakano K, Ng KH, Tomida M, Nagatsuka H, Kawakami T
  • Journal Title: Eur J Med Res 15(Peerreviewed) Pages: 180-184
• [Journal Article] Notch4 overexpression in ameloblastoma correlates with the solid/multicystic phenotype. (2010)
  • Author(s): Siar CH, Nagatsuka H, Chuah KS, Rivera RS, Nakano K, Ng KH, Kawakami T
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110(Peerreviewd) Pages: 224-233
• [Journal Article] Differential expression of Notch receptors and their ligands in desmoplastic ameloblastoma. (2010)
  • Author(s): Siar CH, Nakano K, Han PP, Nagatsuka H, Ng KH, Kawakami T
  • Journal Title: J Oral Pathol Med 39(Peerreviewed) Pages: 552-558
• [Journal Article] Immunolocalization of Notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor. (2010)
  • Author(s): Siar CH, Ha KO, Aung LO, Nakano K, Tsujigiwa H, Nagatsuka H, Ng KH, Kawakami T
  • Journal Title: Eur J Med Res 15(Peerreviewed) Pages: 456-460
• [Journal Article] Immunohistochemical observation of Notch signaling in a case of calcifying cystic odontogenic tumor. (2010)
  • Author(s): Nakano K, Siar CH, Tomida M, Matsuura S, Tsujigiwa H, Nagatsuka H, Kawakani T
  • Journal Title: J Hard Tissue Biol 19(Peerreviewed) Pages: 147-152
  • NAID: 10027422106
• [Journal Article] Immunohistochemical study of Notch signaling proteins in the calcifying epithelial odontogenic tumor (Pindborg tumor). (2010)
  • Author(s): Siar CH, Chuah KS, Nakano K, Rivera RS, Tsujigiwa H, Nagatsuka H, Ng KH, Kawakami T
  • Journal Title: J Hard Tissue Biol 19(Peerreviewed) Pages: 167-174
  • NAID: 10027422168
• [Journal Article] An unsuspected ameloblastoma in the subpontic region of the mandible with consideration of pathogenesis from the radiographic course (2010)
  • Author(s): Siar CH, et al.
  • Journal Title: European Journal of Medical Research Volume: 15 Pages: 153-158
  • Peer Reviewed
• [Journal Article] Squamous odontogenic tumor of the mandible : A case report demonstrating immunoexpression of Notch1,3,4, Jagged1,2 and Delta1 (2010)
  • Author(s): Siar CH, et al.
  • Journal Title: European Journal of Medical Research Volume: 15 Pages: 180-184
  • Peer Reviewed
• [Journal Article] Notch4 overexpression in ameloblastoma correlates with the solid/multicystic phenotype (2010)
  • Author(s): Siar CH, et al.
  • Journal Title: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiolgy and Endodontology Volume: 110 Pages: 224-233
  • Peer Reviewed
• [Journal Article] Differential expression of Notch receptors and their ligands in desmoplastic ameloblastoma (2010)
  • Author(s): Siar CH, et al.
  • Journal Title: Journal of Oral Pathology & Medicine Volume: 39 Pages: 552-558
  • Peer Reviewed
• [Journal Article] Immunohistochemical observation of Notch signaling in a case of calcifying cystic odontogenic tumor (2010)
  • Author(s): Nakano K, et al.
  • Journal Title: Journal of Hard Tissue Biology Volume: 19 Pages: 147-152
  • NAID: 10027422106
  • Peer Reviewed
• [Journal Article] Immunohistochemical study of Notch signaling proteins in the calcifying epithelial odontogenic tumor (Pindborg tumor) (2010)
  • Author(s): Siar CH, et al.
  • Journal Title: Journal of Hard Tissue Biology Volume: 19 Pages: 167-174
  • Peer Reviewed
• [Journal Article] Immunolocalization of Notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor (2010)
  • Author(s): Siar CH, et al.
  • Journal Title: European Journal of Medical Research Volume: 15 Pages: 456-460
  • Peer Reviewed
• [Journal Article] Heparanase and its related molecules in odontogenic tumors. (2009)
  • Author(s): Nagatsuka H, Katase N, Han PP, Tsujigiwa H, Siar CH, Nakajima M, Naomoto Y, Tamamura R, Kawakami T, Gunduz M
  • Journal Title: Oral Med Pathol 13(Peerreviewed) Pages: 81-89
  • NAID: 130004850527
• [Journal Article] Angiogenic squamous dysplasia-like phenomenon in oral epithelial precursor lesions. (2009)
  • Author(s): Siar CH, Oo VPA, Nagatsuka H, Nakano K, Ng KH, Kawakami T
  • Journal Title: Eur J Med Res 14(Peerreviewed) Pages: 315-319
• [Journal Article] Wingless-type protein-1 (Wnt-1) expression in primary conventional and unicystic ameloblastomas and their recurrent tumors. (2009)
  • Author(s): Chuah KS, Siar CH, Nakano K, Nagatsuka H, Khoo SP, Ng KH, Kawakami T
  • Journal Title: J Hard Tissue Biol 18(Peerreviewed) Pages: 63-70
  • NAID: 10025927728
• [Journal Article] Heparanase and its related molecules in odontogenic tumors (2009)
  • Author(s): Nagatsuka H, et al.
  • Journal Title: Oral Medicine and Pathology 13 Pages: 81-89
  • Peer Reviewed
• [Journal Article] Angiogenic squamous dysplasia-like phenomenon in oralepithelial precursor lesions (2009)
  • Author(s): Siar CH, et al.
  • Journal Title: European Journal of Medical Research 14 Pages: 315-319
  • Peer Reviewed
• [Journal Article] Wingless-type protein-1(Wnt-1)expression in primary conventional and unicystic ameloblastomas and their recurrent tumors (2009)
  • Author(s): Chuah KS, et al.
  • Journal Title: Journal of Hard Tissue Biology 18 Pages: 63-70
  • Peer Reviewed
• [Journal Article] A study of recurrent giant odontogenic myxoma of the mandible with immunohistochemical examination of Notch. (2008)
  • Author(s): Nakano K, Chelvanayagam P, Born K, Siar CH, Ng KH, Nagatsuka H, Kawakami T
  • Journal Title: Oral Med Pathol 12(Peerreviewed) Pages: 53-56
  • NAID: 130004850521
• [Journal Article] Notch signaling in benign and malignant ameloblastic neoplasms. (2008)
  • Author(s): Nakano K, Siar CH, Tsujigiwa H, Nagatsuka H, Nagai N, Kawakami T
  • Journal Title: Eur J Med Res 13(Peerreviewed) Pages: 476-480
• [Journal Article] Juxta-epithelial hyalini-zation inhibits tumor growth and invasion in ameloblastoma. (2008)
  • Author(s): Sathi GS, Fujii M, Tmamura R, Borkosky SS, Katase N, Kawakami T, Nagatuska H, Nagai N
  • Journal Title: J Hard Tissue Biol 17(2)(Peerreviewed) Pages: 63-68
• [Journal Article] Immunohistochemical character-ristics of odontogenic neoplasms and their physiological counterparts. (2008)
  • Author(s): Nakano K, Nagatsuka H, Tsujigiwa H, Gunduz M, Katase N, Siar CH, Kawakami T
  • Journal Title: J Hard Tissue Biol 17(3)(Peerreviewed) Pages: 79-90
• [Journal Article] A study of recurrent giant odontogenic myxoma of the mandible with immunohistochemical examination of Notch (2008)
  • Author(s): Nakano K, et al.
  • Journal Title: Oral Medicine and Pathology 12 Pages: 53-56
  • NAID: 130004850521
  • Peer Reviewed
• [Journal Article] Notch signaling in benign and malignant ameloblastic neoplasms (2008)
  • Author(s): Nakano K, et al.
  • Journal Title: European Journal of Medical Research 13 Pages: 476-480
  • Peer Reviewed
• [Journal Article] Juxta-epithelial hyalinization inhibits tumor growth and invasion in ameloblastoma (2008)
  • Author(s): Sathi GS, et al.
  • Journal Title: Journal of Hard Tissue Biology 17 Pages: 63-68
  • Peer Reviewed
• [Presentation] Role of Msx2 as an activator of Runx2 in periodontal tension side by orthodontic mechanical stress (2010)
  • Author(s): Nakano K, et al.
  • Organizer: Asian Science Seminar (6th)
  • Place of Presentation: Taichung, Taiwan
  • Year and Date: 2010-11-20
• [Presentation] Immunolocalization of Notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor (2010)
  • Author(s): Siar CH, et al.
  • Organizer: Annual Scientific Congress of Malaysian Oncological Society
  • Place of Presentation: Kelantan, Malaysia
  • Year and Date: 2010-09-30
• [Presentation] Immunohistchemical expression of Notch signaling in calcifying cystic odontogenic tumor (2010)
  • Author(s): Kawakami T, Siar CH, Nakano K, Tomida M, Matsuura S, Tsujigiwa H, Nagatsuka H
  • Organizer: Biennial Conference of European Association of Oral Medicine (10^<th>) Incorporating the World Workshop on Oral Medicine (Oral Dis 16: 517, 2010)
  • Place of Presentation: London
  • Year and Date: 2010-09-25
• [Presentation] Immunohistchemical expression of Notch signaling in calcifying cystic odontogenic tumor (2010)
  • Author(s): Kawakami T, et al.
  • Organizer: Biennial Conference of European Association of Oral Medicine (10th) Incorporating the World Workshop on Oral Medicine
  • Place of Presentation: London
  • Year and Date: 2010-09-24
• [Presentation] Wnt-antagonist sFRP-2 inhibits bone formation and promotes cell proliferation in ameloblastoma (2010)
  • Author(s): Nagatsuka H, Ara SGS, Katase N, Siar CH, Kawakami T, Tamamura R, Hirata Y, Tsujigiwa H
  • Organizer: International Congress of Oral Pathology and Medicine (15^<th>)(Program and Abstract book p88)
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-08-17
• [Presentation] 石灰化嚢胞性歯原性腫瘍の1例-Notchの免疫組織学的検討- (2010)
  • Author(s): 中野敬介,辻極秀次,長塚仁,長谷川博雅,川上敏行
  • Organizer: 日本臨床口腔病理学会総会(第21回)(プログラム・抄録集 p133)
  • Place of Presentation: 大阪
  • Year and Date: 2010-07-31
• [Presentation] 石灰化嚢胞性歯原性腫瘍の1例-Notchの免疫組織学的検討- (2010)
  • Author(s): 中野敬介, 他
  • Organizer: 日本臨床口腔病理学会総会(第21回)
  • Place of Presentation: 大阪
  • Year and Date: 2010-07-31
• [Presentation] Notch expression patterns and ameloblastoma phenotypes (2010)
  • Author(s): Siar CH, Nakano K, Chuah KS, Nagatsuka H, Ng KH, Kawakami T
  • Organizer: International Association for Dental Research (88^<th>) (J Dent Res 89 (Sp Iss B) Abstract No. 3478, 2010)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-15
• [Presentation] Notch expression patterns and ameloblastoma phenotypes (2010)
  • Author(s): Siar CH, et al.
  • Organizer: International Association for Dental Research (88th)
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-14
• [Presentation] Squamous odontogenic tumorの1症例:Notchとその関連因子の検討 (2010)
  • Author(s): 中野敬介,長塚仁,川上敏行
  • Organizer: 日本病理学会総会(第99回)(日病会誌 99 : 313, 2010)
  • Place of Presentation: 東京
  • Year and Date: 2010-04-28
• [Presentation] Squamous odontogenic tumorの1症例:Notchとその関連因子の検討 (2010)
  • Author(s): 中野敬介, 他
  • Organizer: 日本病理学会総会(第99回)
  • Place of Presentation: 東京
  • Year and Date: 2010-04-28
• [Presentation] Notch receptors and their ligands in ameloblastomas (2009)
  • Author(s): Siar CH, Kawakami T, Nakano K, Nagatsuka H, Ng KH
  • Organizer: 2009 FDI Annual World Dental Congress
  • Place of Presentation: Singapore
  • Year and Date: 2009-09-04
• [Presentation] 口腔粘膜の境界悪性病変におけるデスモゾームタンパク質の異常発現 (2009)
  • Author(s): 相沢聡一,落合隆永,中野敬介,木村晃大,川上敏行,長谷川博雅
  • Organizer: 日本臨床口腔病理学会総会(第20回)(プログラム・抄録集 p86;Oral Med Pathol 14:75, 2009)
  • Place of Presentation: 札幌
  • Year and Date: 2009-07-30
• [Presentation] 口腔粘膜の境界悪性病変におけるデスモゾームタンパク質の異常発現 (2009)
  • Author(s): 相沢聡一, 他
  • Organizer: 日本臨床口腔病理学会総会(第20回)
  • Place of Presentation: 札幌
  • Year and Date: 2009-07-30
• [Presentation] Malaysian Dental Association Annual Dental Meeting (66^<th>) (2009)
  • Author(s): Siar CH, Ng KH, Nagatsuka H, Nakano K, Kawakami T
  • Organizer: Notch signaling molecules in ameloblastoma
  • Place of Presentation: Penang, Malaysia
  • Year and Date: 2009-06-10
• [Presentation] Ameloblastomaとameloblastic carcinomaにおけるHSP27の発現 (2009)
  • Author(s): 中野敬介,川上敏行
  • Organizer: 日本病理学会総会(第98回)(日病会誌 98:360, 2009)
  • Place of Presentation: 京都
  • Year and Date: 2009-05-03
• [Presentation] Ameloblastomaとameloblastic carcinomaにおけるHSP27の発現 (2009)
  • Author(s): 中野敬介, 他
  • Organizer: 日本病理学会総会(第98回)
  • Place of Presentation: 京都
  • Year and Date: 2009-05-03
• [Presentation] 歯胚およびその新生物における基底膜の構造変化とその意義 (2008)
  • Author(s): 中野敬介, 他
  • Organizer: 第21回日本歯科医学会総会
  • Place of Presentation: 横浜市, パシフイコ横浜
  • Year and Date: 2008-11-15
• [Presentation] 歯胚およびその新生物における基底膜の構造変化とその意義 (2008)
  • Author(s): 中野敬介,長塚仁,永井教之,川上敏行
  • Organizer: 日本歯科医学会総会(第21回)(日歯会誌61:111, 2008)
  • Place of Presentation: 横浜
  • Year and Date: 2008-11-14
• [Presentation] 下顎に発生した巨大な歯原性粘液腫の1症例-Notchの免疫組織化学的検討- (2008)
  • Author(s): 中野敬介, 他
  • Organizer: 第19回日本臨床口腔病理学会総会
  • Place of Presentation: 東京都, 水道橋
  • Year and Date: 2008-08-21
• [Presentation] Recurrent giant odontogenic myxoma of the mandible (2008)
  • Author(s): Kawakami T, Nakano K, Chelvanayagam P, Born K, Siar CH, Ng KH
  • Organizer: International congress of oral pathology and medicine (International association of oral pathologists 14^<th>, American academy of oral & maxillofacial pathology 62^<nd>)(Program and abstract p23)
  • Place of Presentation: San Francisco
  • Year and Date: 2008-06-23
• [Presentation] Recurrent giant odontogen ic myxoma of the mandible (2008)
  • Author(s): Kawakami T, et al.
  • Organizer: International Congress of Oral Pathology and Medicine, 14th IAOP, 62nd AAOMP
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2008-06-23
• [Presentation] AmeloblastomaとAmeloblastic CarcinomaにおけるNotchの発現 (2008)
  • Author(s): 中野敬介,長塚仁,永井教之,川上敏行
  • Organizer: 日本病理学会総会(第97回)(日病会誌 97:75, 2008)
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-16
• [Presentation] AmeloblastomaとAmeloblastic CareinomaにおけるNotchの発現 (2008)
  • Author(s): 中野敬介, 他
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢市, 石川県立音楽堂
  • Year and Date: 2008-05-16
• [Book] IMMUNOHISTOCHEMICAL BASIS FOR ORTHODONTIC TREATMENT (2010)
  • Author(s): Kawakami T, et al
  • Total Pages: 361
  • Publisher: Nova Science Publishers, New York, USA.
• [Book] Cell differentiation of neoplastic cells originating in the oral and craniofacial regions (New York, USA.) (2009)
  • Author(s): Kawakami T, Nagatsuka H
  • Publisher: Nova Science Publishers
• [Book] Cell differentiation research developments(in Ivanova LB Ed) (Chapter1 : Cell differentiation of neoplastic cells originating in the oral and craniofacial regions) (New York, USA) (2008)
  • Author(s): Kawakami T, Nagatsuka H, Nakano K, Shimizu T, Tsujigiwa H, Hasegawa H, Nagai N
  • Publisher: Nova Science Publishers
• [Book] Ivanova, LB ed : Cell Differentiation Reserach Developments(第1章分担)(ISBN 13:978-1-60021-938-2 ; 10:1-60021-938-1) (2008)
  • Author(s): Kawakami T, at al.
  • Total Pages: 276
  • Publisher: Nova Science Publishers, Co, New York, USA
• [Book] Cell Differntiation of Neoplastic Cells Originating in the Oral and Craniofacial Regions (ISBN 978-1-60456-932-2) (2008)
  • Author(s): Kawakami T, at al.
  • Publisher: Nova Science Publishers, Co, New York, USA
• [Book] eBook : Cell Differntiation of Neoplastic Cells Originating in the Oral and Caniofacial Regions (ISBN 978-60741-213-7) (2008)
  • Author(s): Kawakami T, at al.
  • Publisher: Nova Science Publishers, Co, New York, USA