Investigation of the mechanisms of osteogenic differentiation by RhoA in periodontal ligament cells for cell transplantation
| Project/Area Number |
20592429
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
YAMAMOTO Tadashi Okayama University, 岡山大学病院, 講師 (50432662)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASHIBA Shogo 岡山大学, 大学院・医歯薬学総合研究科, 教授 (50226768)
MINESHIBA Junji 岡山大学, 岡山大学病院, 講師 (00509383)
YAMASHIRO Keisuke 岡山大学, 大学院・医歯薬学総合研究科, 助教 (30581087)
NARUISHI Koji 岡山大学, 岡山大学病院, 講師 (00346446)
SHIOMI Nobuyuki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (90432632)
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| Co-Investigator(Renkei-kenkyūsha) |
SONOYAMA Wataru 岡山大学, 岡山大学病院, 助教 (40325121)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 歯根膜 / 細胞分化 / RhoA-ROCKシグナル / Y-27632 / 遺伝子導入 / 遺伝子発現 / RhoA / ROCK / Rho / Rho-kinase / ストレスファイバー |
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| Research Abstract |
In periodontal ligament cells (PDL cells), cytoskeletal signaling regulated by Rho-ROCK is critical in the differentiation process, and the expression of osteogenic genes such as BMP-4, Wnt3a, and Wnt5a. Meanwhile over-expression of RhoA or ROCK showed significant low cell proliferation, suggesting that PDL cells differentiation is coordinately regulated by Rho-ROCK induced cytoskeletal molecules, as well as soluble growth factors and extracellular matrix.
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Report
(4 results)
Research Products
(5 results)
