Bovine lactoferrin-based agents which promotes healing of tooth socket

• Project/Area Number: 20592433
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: NAKASHIMA Keisuke Kyushu Dental College, 歯学部, 准教授 (80227785)
• Co-Investigator(Kenkyū-buntansha): ABIKO Yoshihiro 北海道医療大学, 個体差医療科学センター, 教授 (90260819) ; TAKAYAMA Yoshiharu 農業・食品産業技術総合研究機構, 畜産草地研究所, 主任研究員 (00343989)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 外科 / 歯学 / 生体分子 / タンパク質 / 動物 / 蛋白質

Research Abstract

Effects of lactoferrin on healing of tooth socket were evaluated in this investigation. Right lower first molar of 4 weeks-old Wistar male rats was extracted under general anesthesia. Collagen sponge containing controlled-released bovine lactoferrin was inserted in the tooth socket. One week later, the rats were sacrificed. Healing of tooth socket was evaluated with 3-D images and histological images. Results of the investigation showed that controlled-released bovine lactoferrin decreases the number of neutrophils under the gingival epithelium and increase new bone formation.

Research Products

• [Journal Article] Lactoferrin promotes hyaluronan synthesis in human dermal fibroblasts (2011)
  • Author(s): Saito S, Takayama Y, Mizumachi K, Suzuki C
  • Journal Title: Biotechnol Lett 33(1) Pages: 33-39
  • Peer Reviewed
• [Journal Article] Lactoferrin promotes hyaluronan synthesis in human dermal fibroblasts (2011)
  • Author(s): Saito S, Takayama Y, Mizumachi K, Suzuki C
  • Journal Title: Biotechnol Lett Volume: 33(1) Pages: 33-39
  • Peer Reviewed
• [Journal Article] Involvement of adhesion molecule in in vitro plaque-like formation of macrophages stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide (2010)
  • Author(s): Tsutsumi, T., K.Nakashima, T. Isoda, M. Yokota, T. Nishihara
  • Journal Title: J Periodontal Res 45(4) Pages: 550-556
  • Peer Reviewed
• [Journal Article] Nicotine induces upregulated expression of beta defensin -2 via the p38MAPK pathway in the HaCaT human keratinocyte cell line (2010)
  • Author(s): Nakamura S, Saitoh M, Yamazaki M, Nishimura M, Kurashige Y, Arakawa T, Takuma T, Kaku T, Abiko Y
  • Journal Title: Med Mol Morphol 43 Pages: 204-210
  • NAID: 10027752317
  • Peer Reviewed
• [Journal Article] The G1 cell cycle arrest of macrophages infected with Aggregatibacter actinomycetemcomitans (2010)
  • Author(s): Kasai H, 中島啓介, Yokota M, Nishihara T
  • Journal Title: Oral Diseases 16 Pages: 305-309
  • Peer Reviewed
• [Journal Article] Involvement of adhesion molecule in in vitro plaque-like formation of macrophages stimulated with Aggregatibacter actinomycetemcomitans lipo polysaccharide (2010)
  • Author(s): Tsutsumi, T., K.Nakashima, T.Isoda, M.Yokota, T.Nishihara
  • Journal Title: J Periodontal Res Volume: 45(4) Pages: 550-556
  • Peer Reviewed
• [Journal Article] Nicotine induces upregulated expression of beta defensin-2 via the p38 MAPK pathway in the HaCaT human keratinocyte cell line (2010)
  • Author(s): Nakamura S, Saitoh M, Yamazaki M, Nishimura M, Kurashige Y, Arakawa T, Takuma T, Kaku T, Abiko Y.
  • Journal Title: Med Mol Morphol Volume: 43 Pages: 204-210
  • NAID: 10027752317
  • Peer Reviewed
• [Journal Article] The G1 cell cycle arrest of macrophages infected with Aggregatibacter actinomycetemcomitans (2010)
  • Author(s): Kasai H, Nakashima K, Yokota M, Nishihara T
  • Journal Title: Oral Diseases 16 Pages: 305-309
  • Peer Reviewed
• [Journal Article] Expression of claudin-4 and -7 in porcine gingival junctional epithelium (2009)
  • Author(s): Saitoh M, Kurashige Y, Nishimura M, Igarashi S, Kaku T, Abiko Y
  • Journal Title: Medical Molecular Morphology 42 Pages: 212-215
  • NAID: 10027269522
  • Peer Reviewed
• [Journal Article] Effect of lactoferrin-embedded collagen membrane on osteogenic differentiation of human osteoblast-like cells (2009)
  • Author(s): Yoshiharu Takayama, Koko Mizumachi
  • Journal Title: J Biosci Bioeng 107 Pages: 191-195
  • Peer Reviewed
• [Journal Article] Effect oflactoferrin-embedded collagen membrane on osteogenic differentiation of human osteoblast-like cells. (2009)
  • Author(s): Yoshiharu Takayama, Koko Mizumachi
  • Journal Title: J Biosci Bioeng 107 Pages: 191-195
  • Peer Reviewed
• [Journal Article] ヒトラクトフェリンによるエンドトキシン作用の抑制 (2008)
  • Author(s): 中島啓介, 湯本泰弘, 古市保志
  • Journal Title: 歯界展望 112 Pages: 726-727
• [Presentation] ヒトラクトフェジンによるエンドトキシン作用の抑制 (2008)
  • Author(s): 中島啓介, 湯本泰弘, 古市保志
  • Organizer: 第1回歯周病ラクトフェリンフォーラム
  • Place of Presentation: 埼玉
  • Year and Date: 2008-04-27