| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Research Abstract |
A new metamorphosis-enhancing macrodiolide, luminaolide, was isolated from the crustose coralline algae (CCA) Hydrolithon reinboldii. Its structure was determined by spectroscopic analysis. A fraction (1.30 μg/mL) eluted with 80% aqueous MeOH by ODS gel column chromatography of the same CCA extract induced larval metamorphosis (25.9±7.4%) against Leptastrea purpurea, and its metamorphosis-inducing activity was further enhanced to 92.6±2.9% with the addition of 1 (25.6 ng/mL).
Furthermore, eight bromotyrosine derivatives including 11-deoxyfistularine-3 were prepared and tested for their metamorphosis-inducing activity against larvae of scleractinian coral Pseudosiderastrea tayamai. The following structure-activity relationships were noted : (i) two spiroisoxazolin moieties are essential, tyramine derivetive between both spiroisoxazolin moieties is not essential for the activity. (ii) Two hydroxyl groups in each spiroisoxazolin moiety are important for the activity.
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