Screening of specific peptide for tumor cell by chemical biology method.
| Project/Area Number |
20611011
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Okayama University |
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Principal Investigator |
FUTAMI Midori Okayama University, 工学部, 講師 (10467748)
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| Co-Investigator(Kenkyū-buntansha) |
SISIDO Masahiko 岡山大学, 名誉教授 (60026268)
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| Co-Investigator(Renkei-kenkyūsha) |
KITAMATSU Mizuki 岡山大学, 自然科学研究科, 助教 (60379716)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | がん細胞 / ペプチドライブラリー / ペプチドスクリーニング / 蛍光性アミノ酸 / 2次元蛍光スペクトル / スクリーニング / 蛍光 / in vivo imaging |
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| Research Abstract |
Recent studies have revealed the existence of some tumor specific cell surface proteins. Specific peptides for these tumor specific cell surface proteins are available as diagnosis and drug delivery cargo for cancerous tissue. In this study, we have developed a novel efficient method for screening peptides that bind to a target cells. Cancer cells were used directly for screening target in this peptide screening method. During the 3 years we obtained the following results. (1) A431 (epithelial carcinoma cell line) binding peptide was acquired. (2) Tetracycline inducible PSMA (Prostate specific membrane antigen) expression cell line was established. (3) PSMA binding peptide sequence was narrowed down by peptide screening using PSMA expression cell line. These results would contribute for development of this screening method into versatile peptide screening technology.
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Report
(4 results)
Research Products
(14 results)
