Screening of specific peptide for tumor cell by chemical biology method.

• Project/Area Number: 20611011
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Okayama University
• Principal Investigator: FUTAMI Midori Okayama University, 工学部, 講師 (10467748)
• Co-Investigator(Kenkyū-buntansha): SISIDO Masahiko 岡山大学, 名誉教授 (60026268)
• Co-Investigator(Renkei-kenkyūsha): KITAMATSU Mizuki 岡山大学, 自然科学研究科, 助教 (60379716)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: がん細胞 / ペプチドライブラリー / ペプチドスクリーニング / 蛍光性アミノ酸 / 2次元蛍光スペクトル / スクリーニング / 蛍光 / in vivo imaging

Research Abstract

Recent studies have revealed the existence of some tumor specific cell surface proteins. Specific peptides for these tumor specific cell surface proteins are available as diagnosis and drug delivery cargo for cancerous tissue. In this study, we have developed a novel efficient method for screening peptides that bind to a target cells. Cancer cells were used directly for screening target in this peptide screening method. During the 3 years we obtained the following results. (1) A431 (epithelial carcinoma cell line) binding peptide was acquired. (2) Tetracycline inducible PSMA (Prostate specific membrane antigen) expression cell line was established. (3) PSMA binding peptide sequence was narrowed down by peptide screening using PSMA expression cell line. These results would contribute for development of this screening method into versatile peptide screening technology.

Research Products

• [Journal Article] A novel method for screening peptides that bind to proteins by using multiple fluorescent amino acids as fluorescent tags. (2010)
  • Author(s): Kitamatsu M, Futami M, Sisido M
  • Journal Title: Chem Commun (Camb) 46 Pages: 761-763
  • Peer Reviewed
• [Journal Article] Quantitative screening of EGF receptor-binding peptides by using a peptide library with multiple fluorescent amino acids as fluorescent tags. (2010)
  • Author(s): Kitamatsu M, Yamamoto T, Futami M, Sisido M
  • Journal Title: Bioorg Med Chem Lett. 20 Pages: 5976-5978
  • NAID: 120003386547
  • Peer Reviewed
• [Journal Article] A novel method for screening peptides that bind to proteins by using multiple fluorescent amino acids as fluorescent tags. (2010)
  • Author(s): Kitamatsu M, Futami M, Sisido M.
  • Journal Title: Chem Commun (Camb) Volume: 46 Pages: 761-763
  • Peer Reviewed
• [Journal Article] Quantitative screening of EGF receptor-binding peptides by using a peptide library with multiple fluorescent amino acids as fluorescent tags. (2010)
  • Author(s): Kitamatsu M, Yamamoto T, Futami M, Sisido M.
  • Journal Title: Bioorg Med Chem Lett. Volume: 20 Pages: 5976-5978
  • NAID: 120003386547
  • Peer Reviewed
• [Presentation] Screening of the peptides binding specifically to cancer cells (2010)
  • Author(s): 福田隆之
  • Organizer: 第3回高度医療都市を創出する未来技術国際シンポジウム 抗がん剤・抗感染症創薬のための標的分子探求
  • Place of Presentation: 岡山大学大学院自然科学研究科棟
  • Year and Date: 2010-02-03
• [Presentation] Screening of the peptides binding specifically to cancer cells (2010)
  • Author(s): 福田隆之
  • Organizer: 第3回高度医療都市を創出する未来技術国際シンポジウム抗がん剤・抗感染症創薬のための標的分子探求
  • Place of Presentation: 岡山大学大学院自然科学研究科棟
• [Presentation] 蛍光スクリーニング法によるEGFR 結合ペプチドの探索 (2009)
  • Author(s): 山本貴博
  • Organizer: 日本ペプチド学会 第46回ペプチド討論会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2009-11-04
• [Presentation] 蛍光スクリーニング法によるEGFR結合ペプチドの探索 (2009)
  • Author(s): 山本貴博
  • Organizer: 日本ペプチド学会 第46回ペプチド討論会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2009-11-04
• [Presentation] 蛍光クグ法によるEGFR結合ペプチドの開発(3)-EGFR過剰発現細胞A431に対する標的化ペプチドのダイレクトスクリーニング- (2009)
  • Author(s): 福田隆之
  • Organizer: 日本化学会第89春季年会(2009)
  • Place of Presentation: 日本大学理工学部船橋キャンパス
  • Year and Date: 2009-03-27
• [Presentation] 多成分蛍光タグ法によるEGFR結合ペプチドの開発(1)-ペプチド検出のためのプロトコールの確立- (2009)
  • Author(s): 井上圭亮
  • Organizer: 日本化学会第89春季年会(2009)
  • Place of Presentation: 日本大学理工学部船橋キャンパス
  • Year and Date: 2009-03-27
• [Presentation] Screening for EGFR-Binding Peptides by Multi- Component Fluorescent Labeling Method (2008)
  • Author(s): T.Yamamoto
  • Organizer: 第45回ペプチド討論会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2008-10-29
• [Presentation] Multi-component Fluorescence labeling method for Efficient Positional Screening of Peptide Library (2008)
  • Author(s): M.KITAMATSU
  • Organizer: 30th European Peptide Symposium.
  • Place of Presentation: ヘルシンキ
  • Year and Date: 2008-08-31
• [Presentation] Multi-component Fluorescence labeling method for Efficient Positional Screening of Peptide Library (2008)
  • Author(s): M. KITAMATSU
  • Organizer: 30th European Peptide Symposium
  • Place of Presentation: ヘルシンキ
  • Year and Date: 2008-08-31
• [Presentation] 蛍光基を側鎖にもつ非天然アミノ酸群の合成 (2008)
  • Author(s): 北松瑞生
  • Organizer: 第57回高分子学会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2008-05-28