| Budget Amount *help |
¥104,000,000 (Direct Cost: ¥80,000,000、Indirect Cost: ¥24,000,000)
Fiscal Year 2012: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2011: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2010: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2009: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2008: ¥34,580,000 (Direct Cost: ¥26,600,000、Indirect Cost: ¥7,980,000)
|
|---|
| Research Abstract |
enomic imprinting is an epigenetic phenomenon in mammals, in which a subset of genes are mono-allelically expressed depending on their parental origin.To reveal identity of the epigenetic signature discriminating the parental origin and its deposition site within the H19-ICR sequence, transgenic mouse methodology was employed and following findings were demonstrated:
1) Paternal H19-ICR acquired methylation independently of its establishment in sperm
2) CTCF sites were not required to establish paternal-allele-specific methylation in pre-implantation embryos
3) Sequences in the H19-ICR corresponding to the small RNA sequences in oocytes were dispensable for methylation imprinting
4) Differential methylation of the H19-ICR was established by its paternal-allele-specific methylation and maternal-allele-specific protection-against-methylation
5) Oct-sox motif in the H19-ICR harbored protection-against-methylation activity in vivo after implantation
6) Chicken HS4 insulator did not protect the H19-ICR from differential DNA methylation.
|
