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Creation of the 2nd generation peptide aptamer which dynamically controls interface between biomolecule and inorganic surface.

Research Project

Project/Area Number 20681013
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Nanomaterials/Nanobioscience
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SANO Ken-ichi  The Institute of Physical and Chemical Research, 分子情報生命科学特別研究ユニット, 副ユニットリーダー (80321769)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2008: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Keywordsナノバイオ / 分子認識 / ナノ・マイクロデバイス / バイオテクノロジー / アプタマー / ソフト界面 / 蛋白質 / 動的分子素子 / アプタマ
Research Abstract

The present study aims at the creation of a biomolecular element that achieves the dynamic control of binding and the unbinding between an artificial peptide and an inorganic material. I succeeded in making the phage library where the Zn-finger motif frame to screen by combinatorial engineering for the 2nd generation peptide aptamer. I also successfully analyzed in a detailed interfacial structure between a peptide aptamer and inorganic surface. Based on this structure, the design synthesis of the artificial peptide and the artificial protein was carried out.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (50 results)

All 2010 2009 2008 Other

All Journal Article (13 results) (of which Peer Reviewed: 10 results) Presentation (29 results) Book (4 results) Remarks (4 results)

  • [Journal Article] バイオミネラリゼーション研究の新展開-ミネラルの形成と溶解を制御する2010

    • Author(s)
      佐野健一、芝清隆
    • Journal Title

      バイオサイエンスとインダストリー 68

      Pages: 336-340

    • NAID

      10026587058

    • Related Report
      2010 Final Research Report
  • [Journal Article] Autonomous Silica-Encapsulation and Sustained-Release of Anti-Cancer Protein2010

    • Author(s)
      Sano K., Minamisawa, T., Shiba K.
    • Journal Title

      Langmuir 26

      Pages: 2231-2234

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] バイオミネラリゼーション研究の新展開-ミネラルの形成と溶解を制御する2010

    • Author(s)
      佐野健一、芝清隆
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 68 Pages: 336-340

    • NAID

      10026587058

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Autonomous silica-encapsulation and sustained-release of anti-cancer protein2010

    • Author(s)
      Sano K., et al
    • Journal Title

      Langmuir 26(印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Critical amino acid residues for the specific binding of the Ti-recognizing recombinant ferritin with oxide surfaces of titanium and silicon2009

    • Author(s)
      Hayashi T., Sano K., Shiba K., Iwahori K., Yamashita I., Hara M.
    • Journal Title

      Langmuir 25

      Pages: 10901-10906

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hexagonal close-packed array formed by selective adsorption onto hexagonal patterns2009

    • Author(s)
      Matsukawa N., Nishio K., Sano K., Shiba K., Yamashita I.
    • Journal Title

      Langmuir 25

      Pages: 3327-3330

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hexagonal close-packed array formed by selective adsorption onto hexagonal patterns2009

    • Author(s)
      Matsukawa N., et al
    • Journal Title

      Langmuir 25

      Pages: 3327-3330

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Critical amino acid residues for the specific binding of the Ti-recognizing recombinant ferritin with oxide surfaces of titanium and silicon2009

    • Author(s)
      Hayashi T., et al
    • Journal Title

      Langmuir 25

      Pages: 10901-10906

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stepwise accumulation of layers of aptamer-ornamed ferritins using Biomimetic Layer-By-Layer2008

    • Author(s)
      Sano K., Shiba K.
    • Journal Title

      J Mater Res 23

      Pages: 3236-3240

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] In aqua manufacturing of three-dimensional nanostructure using a peptide aptamer2008

    • Author(s)
      Sano K., Shiba K.
    • Journal Title

      MRS Bulltein 33

      Pages: 524-529

    • Related Report
      2010 Final Research Report
  • [Journal Article] Stepwise accumulation of layers of aptamer-ornamed ferritins using Biomimetic Layer-By-Layer2008

    • Author(s)
      Ken-Ichi Sano, et al.
    • Journal Title

      Journal of Material Research 23

      Pages: 3236-3240

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In aqua manufacturing of three-dimensional nanostructure using a paptide aptamer2008

    • Author(s)
      Ken-Ichi Sano, et al.
    • Journal Title

      MRS Bulltein 33

      Pages: 524-529

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermoresponsive Microtubule Hydrogel with High Hierarchical Structure

    • Author(s)
      Sano K., Kawamura R., Tominaga T., Nakagawa H., Oda N., Ijiro K., Osada Y.
    • Journal Title

      Biomacromolecules 印刷中

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] Strengthening of the Hydrogel with Porous Inorganic Particles2010

    • Author(s)
      Hideyuki Mitomo, et al.
    • Organizer
      Pacifichem 2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高次の階層構造を持つ細胞骨格タンパク質の材料化2010

    • Author(s)
      佐野健一
    • Organizer
      新規材料創製を目指した合成生物学
    • Place of Presentation
      和光(招待講演)
    • Year and Date
      2010-11-19
    • Related Report
      2010 Final Research Report
  • [Presentation] Reversible gel-sol transition of covalently cross-linked actin hydrogel2010

    • Author(s)
      Ken-Ichi Sano, et al.
    • Organizer
      日本生物物理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高次の階層構造を持つ細胞骨格タンパク質ハイドロゲルの創発機能2010

    • Author(s)
      佐野健一
    • Organizer
      統合バイオナノシステム研究がもたらすイノベーション基盤の新展開
    • Place of Presentation
      和光
    • Year and Date
      2010-08-07
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Reversible Gel-Sol transition of Actin gel2010

    • Author(s)
      Sano K, Kawamura R., Tominaga T., Oda N., Ijiro K., Osada Y.
    • Organizer
      in Polymer Physics 2010
    • Place of Presentation
      Ji-nan
    • Year and Date
      2010-06-07
    • Related Report
      2010 Final Research Report
  • [Presentation] Reversible Gel-Sol Transition of Actin Gel2010

    • Author(s)
      Ken-Ichi Sano, et al.
    • Organizer
      Polymer Physics 2010
    • Place of Presentation
      Ji-nan, China
    • Year and Date
      2010-06-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Reversible Gel-Sol Transition of Actin Gel2010

    • Author(s)
      Ken-Ichi Sano, et al.
    • Organizer
      高分子学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2010-05-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNA gel cross-linked by intercalation2010

    • Author(s)
      Ken-Ichi Sano, et al.
    • Organizer
      高分子学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2010-05-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Strengthening of the Hydrogel with Inorganic Particles2010

    • Author(s)
      Hideyuki Mitomo, et al.
    • Organizer
      高分子学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2010-05-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高次の階層構造を持つ細胞骨格タンパク質の材料化2010

    • Author(s)
      佐野健一
    • Organizer
      新規材料創製を目指した合成生物学
    • Place of Presentation
      横浜
    • Related Report
      2010 Annual Research Report
  • [Presentation] Towards developing a novel biomachine with reversible 3D-crosslinked 'bio-hydrogel'2009

    • Author(s)
      Sano K, Kawamura R., Tominaga T., Oda N., Ijiro K., Osada Y.
    • Organizer
      in 4^<th> Conference on Artificial Muscles/5^<th> World Congress on Biomimetics, Artificial Muscles and Nano-Bio
    • Place of Presentation
      大阪
    • Year and Date
      2009-11-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Towards developing a novel biomachine with reversible 3D-crosslinked 'bio-hydrogel2009

    • Author(s)
      佐野健一, et al.
    • Organizer
      4^<th> Conference on Artificial Muscles / 5^<th> World Congress on Biomimetics, Artificial Muscles and Nano-Bio
    • Place of Presentation
      大阪府豊中
    • Year and Date
      2009-11-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 微小管による温度可逆的なハイドロゲルの創製2009

    • Author(s)
      川村隆三, 他
    • Organizer
      日本生物物理学会第47回年会
    • Place of Presentation
      徳島県徳島
    • Year and Date
      2009-11-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] 人工ペプチドの機能と半導体メモリーの接点2009

    • Author(s)
      佐野健一
    • Organizer
      CREST「生体超分子援用フロンティアプロセスによる高機能化ナノシステム」研修会
    • Place of Presentation
      湯河原(招待講演)
    • Year and Date
      2009-08-11
    • Related Report
      2010 Final Research Report
  • [Presentation] 人工ペプチドの機能と半導体メモリーの接点2009

    • Author(s)
      佐野健一
    • Organizer
      「生体超分子援用フロンティアプロセスによる高機能化ナノシステム」研修会
    • Place of Presentation
      神奈川県湯河原
    • Year and Date
      2009-08-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] ナノカーボン標的ペプチドアプタマーの自己組織化を利用した球状タンパク質の繊維化2009

    • Author(s)
      佐野健一, 他
    • Organizer
      理研シンポジウム「階層横断生命科学」
    • Place of Presentation
      (理化学研究所)埼玉県和光
    • Year and Date
      2009-08-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] ペプチドアプタマーの自己組織化を利用した球状タンパク質の繊維化2009

    • Author(s)
      佐野健一, 長田義仁, 佐々木博之, 加瀬大介, 芝清隆
    • Organizer
      平成21年度繊維学会年次大会
    • Place of Presentation
      東京
    • Year and Date
      2009-06-11
    • Related Report
      2010 Final Research Report
  • [Presentation] ペプチドアプタマーの自己組織化を利用した球状タンパク質の繊維化2009

    • Author(s)
      佐野健一, 他
    • Organizer
      繊維学会年次大会
    • Place of Presentation
      東京江戸川区
    • Year and Date
      2009-06-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] In Aqua Miniaturization of a 3-Dimensional Nano-Structure and Semi-conductor Memory Device Using a Peptide Aptamer2008

    • Author(s)
      Ken-Ichi Sano
    • Organizer
      分子情報生命科学シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] 生体分子の自己組織化能を利用したデバイス開発における分子生物学の役割2008

    • Author(s)
      佐野健一
    • Organizer
      BMB2008シンポジウム"分子生物学からポストナノテクノロジーに向けて"
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 生体分子の自己組織化能を利用したデバイス開発における分子生物学の役割2008

    • Author(s)
      佐野 健一
    • Organizer
      BMB2008 シンポジウム 分子生物学からポストナノテクノロジーに向けて
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Utilization of Biological Molecular Recognition Enables Biomimetic Nanofabrication of Metal Oxide Semiconductor Transistor Device2008

    • Author(s)
      Sano K.
    • Organizer
      10th RIES symposium
    • Place of Presentation
      札幌(招待講演)
    • Year and Date
      2008-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Utilization of Biological Molecular Recognition Enables Biomimetic Nanofabrication of Metal Oxide Semiconductor Transistor Device2008

    • Author(s)
      Ken-Ichi Sano
    • Organizer
      10th RIES symposium
    • Place of Presentation
      札幌
    • Year and Date
      2008-12-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] マテリアルとバイオをインターフェイスするペプチドアプタマー2008

    • Author(s)
      佐野健一、芝清隆
    • Organizer
      日本生物工学会年会
    • Place of Presentation
      仙台(招待講演)
    • Year and Date
      2008-08-28
    • Related Report
      2010 Final Research Report
  • [Presentation] マテリアルとバイオをインターフェイスするペプチドアプタマー2008

    • Author(s)
      佐野 健一, 他
    • Organizer
      日本生物工学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2008-08-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 人工ペプチドの分子認識はデバイス開発にどこまで使えるか?2008

    • Author(s)
      佐野健一
    • Organizer
      分子研シンポジウム
    • Place of Presentation
      岡崎(招待講演)
    • Year and Date
      2008-08-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 人工ペプチドの分子認識はデバイス開発にどこまで使えるか?2008

    • Author(s)
      佐野 健一
    • Organizer
      分子研シンポジウム
    • Place of Presentation
      岡崎
    • Year and Date
      2008-08-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] ナノバイオテクノロジーにおけるQCM-D技術の利用2008

    • Author(s)
      佐野健一
    • Organizer
      QCM-Dユーザーミーティング
    • Place of Presentation
      東京(招待講演)
    • Year and Date
      2008-06-09
    • Related Report
      2010 Final Research Report
  • [Presentation] ナノバイオテクノロジーにおけるQCM-D技術の利用2008

    • Author(s)
      佐野 健一
    • Organizer
      QCM-D ユーザーミーティング
    • Place of Presentation
      東京
    • Year and Date
      2008-06-09
    • Related Report
      2008 Annual Research Report
  • [Book] 『バイオアクチュエータとしての細胞骨格トレッドミルマシン』未来を動かすソフトアクチュエータ-高分子・生体材料を中心とした研究開発-2010

    • Author(s)
      佐野健一、川村隆三、長田義仁
    • Publisher
      シーエムシー出版
    • Related Report
      2010 Final Research Report
  • [Book] 未来を動かすソフトアクチュエータ-高分子・生体材料を中心とした研究開発-2010

    • Author(s)
      佐野健一, 他
    • Publisher
      シーエムシー出版
    • Related Report
      2010 Annual Research Report
  • [Book] 『ゲノムからナノバイオへの取り組み』実践ゲノムの最前線2009

    • Author(s)
      佐野健一
    • Publisher
      六然社
    • Related Report
      2010 Final Research Report
  • [Book] 実践ゲノムの最前線(分担)ゲノムからナノバイオへの取り組み2009

    • Author(s)
      佐野 健一
    • Total Pages
      12
    • Publisher
      六然社
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.riken.jp/msls/member/Sano.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.riken.jp/msls/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.riken.jp/msls/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.riken.jp/msls/

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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