Functional analysis of RNA-binding protein Musashi2 in the nervous system

• Project/Area Number: 20700297
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Keio University
• Principal Investigator: KUWAKO Ken-ichiro Keio University, 医学部, 助教 (30468475)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: RNA結合蛋白質 / 神経回路形成 / 神経科学 / 発生分化 / 発生・分化

Research Abstract

We demonstrate that the expression of Slit receptor Robo3 is regulated by a neural RNA-binding protein Musashi (Msi). Msi binds to Robo3 mRNA and increases its translation. In Msi-deficient precerebellar neurons, Robo3 protein is dramatically reduced. Moreover, axonal midline crossing of precerebellar neurons are severely impaired in Msi-deficient mice.

Research Products

• [Journal Article] Neural RNA-binding protein Musashil controls midline crossing of precerebellar neurons through post-transcriptional regulation of Robo3/Rig-1 expression (2010)
  • Author(s): Kuwako K., Kakumoto K., Imai T., Igarashi M., Hamakubo T., Sakakibara S., Tessier-Lavigne M., Okano H.J., Okano H.
  • Journal Title: Neuron 67 Pages: 407-421
  • Peer Reviewed
• [Journal Article] RNA結合タンパク質MusashilによるRobo3の転写後発現制御を介した軸索誘導機構 (2010)
  • Author(s): 桑子賢一郎、岡野栄之
  • Journal Title: 文部科学省委託研究開発事業「統合データベースプロジェクト」ライフサイエンス新着論文レビュー(オンラインレビュー)
  • Peer Reviewed
• [Journal Article] Neural RNA-binding protein Musashil controls midline crossing of precerebellar neurons through post-transcriptional regulation of Robo3/Rig-1 expression. (2010)
  • Author(s): Kuwako K, et al.
  • Journal Title: Neuron Volume: 67 Pages: 407-421
  • Peer Reviewed
• [Presentation] Robo3転写後調節による新規軸索誘導機構 (2010)
  • Author(s): 桑子賢一郎
  • Organizer: 第33回日本神経科学会・第53回日本神経化学会(NEURO2010)
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-02
• [Presentation] A possible mechanism for post-transcriptional regulation in axon guidance (2010)
  • Author(s): 桑子賢一郎、岡野栄之
  • Organizer: 第4回神経発生討論会
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2010-03-20
• [Presentation] 神経軸索誘導における転写後調節の重要性 (2010)
  • Author(s): 桑子賢一郎、岡野栄之
  • Organizer: 第6回慶應義塾大学先端科学技術シンポジウムこころを生み出す神経基盤の解明
  • Place of Presentation: 東京・慶應義塾大学
  • Year and Date: 2010-01-26
• [Presentation] A role for RNA-binding protein Musashil in regulating the axonal midline crossing (2009)
  • Author(s): Ken-ichiro Kuwako, Hideyuki Okano
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto
  • Year and Date: 2009-07-30
• [Presentation] A role for RNA-binding protein Musashil in regulating the axonalmidline crossing (2009)
  • Author(s): Ken-ichiro Kuwako, Hideyuki Okano
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: Kyoto
  • Year and Date: 2009-07-30
• [Presentation] A possible mechanism for post-transcriptional regulation in the axon guidance (2009)
  • Author(s): 桑子賢一郎
  • Organizer: 第38回慶應ニューロサイエンス研究会
  • Place of Presentation: 東京・慶應義塾大学
  • Year and Date: 2009-03-14
• [Presentation] A possible mechanism for post-transcriptional regulation in the axon guidance (2009)
  • Author(s): Ken-ichiro Kuwako, Hideyuki Okano
  • Organizer: Keystone symposia 2009
  • Place of Presentation: Keystone, Colorado, USA
  • Year and Date: 2009-02-19
• [Presentation] A possible mechanism for post-transcriptional regulation in the axon guidance (2009)
  • Author(s): Ken-ichiro Kuwako, Hideyuki Okano
  • Organizer: Keystone symposia 2009 : Axonal Connections
  • Place of Presentation: Keystone, Corolado
  • Year and Date: 2009-02-19
• [Presentation] RNA-binding protein Musashi regulates the midline crossing of precerebellar neurons. (2009)
  • Author(s): 桑子賢一郎、岡野栄之
  • Organizer: JOINT FORUM(京大再生研、熊大発生研、慶應義塾大学・医、理化学研究所CDB)
  • Place of Presentation: 熊本大学発生研、グリーンピア南阿蘇
  • Year and Date: 2009-01-07
• [Presentation] RNA-binding protein Musashi regulates the midline crossing of precerebellar neurons. (2008)
  • Author(s): 桑子賢一郎、岡野栄之
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] RNA-binding protein Musashi regulates the midline crossing of Drecerebellar neurons (2008)
  • Author(s): 桑子賢一郎、岡野栄之
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] A possible mechanism for post-transcriptional regulation in axon guidance (2008)
  • Author(s): Ken-ichiro Kuwako
  • Organizer: The International Discussion Forum & the 242^<nd> Tokyo Discussion Meeting of the Physiological Society of Japan(第242回日本生理学会東京談話会)
  • Place of Presentation: Tokyo
  • Year and Date: 2008-10-26
• [Presentation] The role for RNA-binding protein Musashi in regulating the development of precerebellar neurons (2008)
  • Author(s): Ken-ichiro Kuwako, Hideyuki Okano
  • Organizer: Cold Spring Harbor Laboratory meeting 2008 "Axon Guidance, Synaptogenesis & Neural Plasticity"
  • Place of Presentation: Cold Spring Harbor Laboratory, NY, USA
  • Year and Date: 2008-09-12
• [Presentation] The role for RNA-binding protein Musashi in regulating the development of precerebellar neurons (2008)
  • Author(s): Ken-ichiro Kuwako, Hideyuki Okano
  • Organizer: Cold Spring Harbor Laboratory meeting 2008 "Axon Guidance, Synaptogenesis & Neural Plasticity"
  • Place of Presentation: Cold Spring Harbor Laboratory, NY
  • Year and Date: 2008-09-12
• [Presentation] 小脳におけるRNA結合蛋白質Musashi2の機能解析 (2008)
  • Author(s): 桑子賢一郎、芝田晋介、岡野ジェームス洋尚、岡野栄之
  • Organizer: 第31回日本神経科学会・第51回日本神経化学会(NEURO2008)
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Presentation] 小脳におけるRNA結合蛋白質Musashi2の機能解析 (2008)
  • Author(s): 桑子腎一郎、岡野栄之
  • Organizer: 第31回日本神経科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-09
• [Remarks] ホームページ等 なし