Analysis for the roles of endocytosis in neuronal migration and morphological changes during cerebral cortical development

• Project/Area Number: 20700317
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Keio University
• Principal Investigator: KAWAUCHI Takeshi Keio University, 医学部, 講師 (60397544)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 神経発生 / 分化 / 異常 / 大脳皮質 / マウス / 神経細胞移動 / Dynamin / Cdk5

Research Abstract

Neuronal migration has essential roles for cerebral cortical development. Although many studies have focused on the important roles of cytoskeletal proteins, the requirement of membrane trafficking-related proteins has remained unclear. In this study, we revealed that Dynamin, a central regulator for endocytosis, is involved in cortical neuronal migration in vivo.

Research Products

• [Journal Article] Regulation of cell adhesion and migration in cortical neurons : not only Rho but also Rab family small GTPases (2011)
  • Author(s): Kawauchi T.
  • Journal Title: Small GTPases Vol.2(1) in press.
  • Peer Reviewed
• [Journal Article] Regulation of cell adhesion and migration in cortical neurons : not only Rho but also Rab family small GTPases (2011)
  • Author(s): Kawauchi T
  • Journal Title: Small GTPases Volume: 2 Pages: 36-40
  • Peer Reviewed
• [Journal Article] Role of dual leucine zipper-bearing kinase (DLK/MUK/ZPK) in axonal growth (2010)
  • Author(s): Eto K, Kawauchi T, Osawa M, Tabata H, Nakajima K.
  • Journal Title: Neurosci. Res. Vol.66(1) Pages: 37-45
  • Peer Reviewed
• [Journal Article] Role of dual leucine zipper-bearing kinase (DLK/MUK/ZPK) in axonal growth (2010)
  • Author(s): Eto K, Kawauchi T, Osawa M, Tabata H, Nakajima K.
  • Journal Title: Neurosci. Res. 66 Pages: 37-45
  • Peer Reviewed
• [Journal Article] Rac is involved in the interkinetic nuclear migration of cortical progenitor cells' (2009)
  • Author(s): Minobe S, Sakakibara A, Ohdachi T, Kanda R, Kimura M, Nakatani S, Tadokoro R, Ochiai W, Nishizawa Y, Mizoguchi A, Kawauchi T, Miyata T.
  • Journal Title: Neurosci. Res. Vol.63(4) Pages: 294-301
  • Peer Reviewed
• [Journal Article] 細胞内現象からみた大脳皮質形成のメカニズム-"in vivo細胞生物学"による神経発生研究の展開- (2008)
  • Author(s): 川内健史
  • Journal Title: 蛋白質核酸酵素 53 Pages: 1957-1967
  • NAID: 40016350944
  • Peer Reviewed
• [Journal Article] Rac is involved in the interkinetic nuclear migration of cor tical progenitor cells (2008)
  • Author(s): Minobe S, Sakakibara A, Ohdachi T, Kanda R, Kimura M, Nakatani S, Tadokoro R, Ochiai W, Nishizawa Y, Mizoguchi A, Kawauchi T, Miyata T.
  • Journal Title: Neurosci. Res 63 Pages: 294-301
  • Peer Reviewed
• [Presentation] 発生期大脳皮質における神経細胞の移動と成熟の分子機構 (2010)
  • Author(s): 川内健史
  • Organizer: 神戸大学大学院医学研究科大学院講義
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-03
• [Presentation] 発生期大脳皮質における神経細胞の移動と成熟の分子機構 (2010)
  • Author(s): 川内健史
  • Organizer: 神戸大学大学院医学研究科 大学院講義
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-03
• [Presentation] In vivo細胞生物学による神経細胞移動のメカニズムの解明 (2010)
  • Author(s): 川内健史
  • Organizer: 第28回脳科学グローバルCOE若手フォーラム
  • Place of Presentation: 東北大学、仙台
  • Year and Date: 2010-09-24
• [Presentation] In vivo細胞生物学による神経細胞移動のメカニズムの解明 (2010)
  • Author(s): 川内健史
  • Organizer: 第28回 脳科学グローバルCOE 若手フォーラム
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-24
• [Presentation] Molecular and cellular mechanisms for the radial glial fiber-dependent locomotion mode of cortical neuronal migration (2010)
  • Author(s): Takeshi Kawauchi
  • Organizer: Global COE Liaison Laboratory regular seminar
  • Place of Presentation: Kumamoto University, Kumamoto, Japan.
  • Year and Date: 2010-09-15
• [Presentation] Molecular and cellular mechanisms for the radial glial fiber-dependent locomotion mode of cortical neuronal migration (2010)
  • Author(s): Takeshi Kawauchi
  • Organizer: Global COE Liaison Laboratory regular seminar
  • Place of Presentation: 熊本
  • Year and Date: 2010-09-15
• [Presentation] エンドサイトーシス経路によるN-カドヘリンの細胞内輸送を介した神経細胞移動の制御機構 (2010)
  • Author(s): 川内健史、関根克敏、鹿内弥磨、富田憲司、久保健一郎、鍋島陽一、星野幹雄、仲嶋一範
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(BMB2010)、一般口頭発表「細胞の構造と機能/Cell Biology」
  • Place of Presentation: 神戸
• [Presentation] 大脳皮質形成過程におけるRap1依存的な神経細胞移動の局面 (2010)
  • Author(s): 関根克敏、川内健史、久保健一郎、本田岳夫、仲嶋一範
  • Organizer: 第33回日本神経科学大会・第53回日本神経科学会大会・第20回日本神経回路学会大会合同大会(Neuro2010)
  • Place of Presentation: 神戸
• [Presentation] Molecular mechanisms of Reelin-Dab1 signaling in the mammalian neocortical development (2010)
  • Author(s): Katsutoshi Sekine, Takeshi Kawauchi, Ken-ichiro Kubo, Takao Honda, Kazunori Nakajima
  • Organizer: Global COE program Center for Human Metabolomic Systems Biology Workshop
  • Place of Presentation: Yokohama
• [Presentation] エンドサイトーシス経路によるN-カドヘリンの細胞内輸送を介した神経細胞移動の制御機構 (2010)
  • Author(s): 川内健史、関根克敏、鹿内弥磨、富田憲司、久保健一郎、鍋島陽一、星野幹雄、仲嶋一範
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会(BMB2010)
  • Place of Presentation: 神戸
• [Presentation] Molecular mechanisms of Reelin-Dab1 signaling in the mammalian neocortical development (2010)
  • Author(s): Katsutoshi Sekine, Takeshi Kawauchi, Ken-ichiro Kubo, Takao Honda, Kazunori Nakajima
  • Organizer: Global COE program Center for Human Metabolomic Systems Biology Workshop
  • Place of Presentation: 横浜
• [Presentation] 大脳皮質形成の分子機構の解明に向けた"in vivo細胞生物学"的アプローチ (2009)
  • Author(s): 川内健史
  • Organizer: 名古屋大学大学院医学研究科基礎医学特論;名古屋大学大学院医学研究科
  • Place of Presentation: 愛知
  • Year and Date: 2009-06-05
• [Presentation] 大脳皮質形成の分子機構の解明に向けた"in vivo細胞生物学"的アプローチ (2009)
  • Author(s): 川内健史
  • Organizer: 名古屋大学大学院医学研究科基礎医学特論
  • Place of Presentation: 名古屋大学大学院医学研究科、愛知
  • Year and Date: 2009-06-05
• [Presentation] 細胞骨格関連分子による神経細胞の移動と形態変化の制御 (2009)
  • Author(s): 川内健史
  • Organizer: 2008年度発達障害研究所共同セミナー;愛知県心身障害者コロニー発達障害研究所
  • Place of Presentation: 愛知
  • Year and Date: 2009-01-09
• [Presentation] 細胞骨格関連分子による神経細胞の移動と形態変化の制御 (2009)
  • Author(s): 川内健史
  • Organizer: 2008年度発達障害研究所共同セミナー
  • Place of Presentation: 愛知県心身障害者コロニー発達障害研究所
  • Year and Date: 2009-01-09
• [Presentation] 大脳皮質2/3層錐体細胞の放射状移動を制御する新たなカルシウムエフェクターCaMKIαの機能解明 (2009)
  • Author(s): 安達-森島亜希, 竹本-木村さやか, 鈴木敢三, 上田(石原)奈津実, 野中美応, 岡村理子, 西村嘉晃, 川内健史, 仲嶋一範, 奥野浩行, 尾藤晴彦
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] A pivotal role of a CaMKK-Ca2+/calmodulin-dependent protein kinase I cascade in the radial migration of layer 2/3 cortical pyramidal neurons (2009)
  • Author(s): Takemoto-Kimura S., Adachi-Morishima A., Ageta-Ishihara N., Suzuki K., Nonaka M., Okamura M., Nishimura Y.V., Kawauchi T., Nakajima K., Okuno H., Bito H.
  • Organizer: Society for Neuroscience, Neuroscience 2009 Mceting (39th Annual Mceting)
  • Place of Presentation: Chicago, U.S.A.
• [Presentation] Cdk5-p27^<kip1> pathway in cortical neuronal migration and morphological changes (2009)
  • Author(s): Takeshi Kawauchi
  • Organizer: 平成21年度二国間交流事業協同セミナー「Cdk5 International Symposium」
  • Place of Presentation: Tokyo Metropolitan University, Tokyo, Japan.
• [Presentation] Cdk5-p27^<kip1> pathway in cortical neuronal migration and morphological changes (2009)
  • Author(s): Takeshi Kawauchi
  • Organizer: 2^<nd> Cdk5 International Symposium
  • Place of Presentation: 首都大学、東京
• [Presentation] 大脳皮質2/3層錐体細胞の放射状移動を制御する新たなカルシウムエフェクターCaMKIαの機能解明 (2009)
  • Author(s): 安達一森島亜希, 竹本一木村さやか, 鈴木敢三, 上田(石原), 奈津実, 野中美応, 岡村理子, 西村嘉晃, 川内健史, 仲嶋一範, 奥野浩行, 尾藤晴彦
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場など、神戸
• [Presentation] A pivotal role of a CaMKK-Ca2+/calmodulin-dependent protein kinase I caseade in the radial migration of layer 2/3 cortical pyramidal neurons (2009)
  • Author(s): Tahemoto-Kimura S., Adachi-Morishima A., Ageta-lshihara N., Suzuki K., Nonaka M., Okamura M., Nishimura Y.V., Kawauchi T., Nakajima K., Okuno H., Bito H.
  • Organizer: Society for Neuroscience, Neuroscience 2009 Meeting (39th Annual Meeting)
  • Place of Presentation: Chicago, U.S.A.
• [Presentation] In vivo cell biology for cerebral cortical development (2008)
  • Author(s): Takeshi Kawauchi
  • Organizer: RIKEN BSI (Brain Science Institute) Informal Seminar
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 2008-10-06
• [Presentation] In vivo cell biology for cerebral cortical development (2008)
  • Author(s): Kawauchi T
  • Organizer: RIKEN BSI Informal Seminar
  • Place of Presentation: 理研BSI(東京都)
  • Year and Date: 2008-10-06
• [Presentation] 神経細胞特異的な移動様式であるロコモーション移動を制御する分子機構の解析 (2008)
  • Author(s): 西村嘉晃、仲嶋一範、鍋島陽一、星野幹雄、川内健史
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 神戸
• [Presentation] Chemical inhibitor screening for molecules regulating morphological changes of locomoting neurons in the developing cerebral cortex (2008)
  • Author(s): Yoshiaki V.Nishimura, Kazunori Nakajima, Yo-ichi Nabeshima, Mikio Hoshino, Takeshi Kawauchi
  • Organizer: 第50回日本神経化学会大会、Oral Session「軸索輸送、細胞運動、細胞骨格」
  • Place of Presentation: 富山
• [Presentation] Search for molecules regulating morphological changes of migrating neurons using real-time imaging of cortical slices (2008)
  • Author(s): Yoshiaki V.Nishimura, Kazunori Nakajima, Mikio Hoshino, Yo-ichi Nabeshima, Takeshi Kawauchi
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] Possible involvement of Cdk5 and JNK in the migration and morphological changes of locomoting neurons during cerebral co rtical development (2008)
  • Author(s): Nishimura YV, Nakajima K, N abeshima YI, Hoshino M, Kaw auchi T
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 兵庫県神戸市
• [Presentation] Chemical inhibitor screening for molecules regulating morpho logical changes of locomoting neurons in the developing cere bral cortex (2008)
  • Author(s): Nishimura YV, Nakajima K, N abeshima YI, Hoshino M, Kaw auchi T
  • Organizer: 第50回日本神経化学会大会
  • Place of Presentation: 富山県
• [Presentation] Search for molecules regulating morphological changes of mig rating neurons using real-time imaging of cortical slices (2008)
  • Author(s): Nishimura YV, Nakajima K, oshino M, Nabeshima YL Kaw auchi T
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京都
• [Remarks] ホームページ等
  • URL: http://web.sc.itc.keio.ac.jp/anatomy/nakajima/page10/page17/page17.html
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