| Project/Area Number |
20710148
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎ゲノム科学
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| Research Institution | Keio University |
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Principal Investigator |
TATEYAMA Seiji Keio University, 理工学研究科, 特別研究講師 (90338202)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 転写制御因子 / DNA / 結合タンパク質 / 相互作用 / mRNAディスプレイ / 転写制御領域 / 発現制御機構 / 複合体 / モチーフ構造 / DNA結合タンパク質 |
|---|
| Research Abstract |
Since the specific interactions between cis-regulatory DNA elements and transcription factors are critical components of transcriptional regulatory networks, comprehensive analysis of DNA-transcription factor interactions is expected to provide a deep understanding of the mechanisms of cell proliferation, developmental processes in tissue morphogenesis, and disease. In this research program, I have shown for the first time that in vitro virus (IVV) selection system, an mRNA display technique, can be employed for the elucidation of new, biologically significant DNA-protein interactions. The IVV system described herein will facilitate the large-scale analysis of DNA-transcription factor interactions for mapping of transcriptional regulatory networks on a genome-wide scale.
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