Establishment of genome-wide analysis of post-translational modified proteins binding sites in mammalian cells
Project/Area Number |
20710150
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
基礎ゲノム科学
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
KUBOSAKI Atsutaka The Institute of Physical and Chemical Research, LSAシステム構築ユニット, 研究員 (30425673)
|
Project Period (FY) |
2008 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | ChIP-chip / 転写因子 / ユビキチン様蛋白質 / IRF8 / IFN刺激応答配列 / ISG15 / 翻訳後修飾 / 転写制御 |
Research Abstract |
ISG15, an ubiquitin-like molecule, knockdown THP-1 myelomonocytic leukemia cells were induced cell death following PMA stimulation. ISG15 gene promoter region contains an interferon response element. Since interferon regulatory factor 8 (IRF8) is a transcription factor expressed predominantly in THP-1 cells, IRF8 was thought to play a role of gene regulation of ISG15. To find the functional direct target genes of IRF8, the gene expression profiles of siRNA knockdown samples and genome-wide binding locations by ChIP-chip were analyzed in THP-1 cells. Consequently, ISG15 and other 84 genes were identified as functional direct targets of IRF8.
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Report
(3 results)
Research Products
(3 results)
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[Journal Article] The combination of gene perturbation assay and ChIP-chip reveals functional direct target genes for IRF8 in THP-1 cells.2010
Author(s)
Kubosaki, A., Lindgren, G., Tagami, M., Simon, C., Tomaru, Y., Miura, H., Suzuki, T., Arner, E., Forrest, A.R.R., Irvine, K.M., Schroder, K., Hasegawa, Y., Kanamori-Katayama, M., Rehli, M., Hume, D.A., Kawai, J., Suzuki, M., Suzuki, H., Hayashizaki, Y.
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Journal Title
Related Report
Peer Reviewed
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[Presentation] The combination of ChIP-chip and gene perturbation assay reveals high confidence target genes for ICSBP and PU. 1 in THP-1 cells.2009
Author(s)
Kubosaki, A., Tagami, M., Tomaru, Y., Simon, C., Suzuki, M., Suzuki, H., Hayashizaki, Y
Organizer
The 32nd Annual Meeting of MBSJ
Place of Presentation
Yokohama, Kanagawa, Japan
Year and Date
20091209-20091212
Related Report
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[Presentation] The combination of ChIP-chip and gene perturbation assay reveals high confidence target genes for ICSBP and PU.1 in THP-1 cells2009
Author(s)
Kubosaki, A., Tagami, M., Tomaru, Y., Simon, C., Suzuki, M., Suzuki, H., Hayashizaki, Y
Organizer
第32回日本分子生物学会年会
Place of Presentation
横浜
Year and Date
2009-12-12
Related Report