Development of RNA drugs based on artificial microRNAs

• Project/Area Number: 20710157
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: ゲノム情報科学
• Research Institution: Kyushu University
• Principal Investigator: SHIBATA Atsushi Kyushu University, 大学院・医学研究院, 学術研究員 (40467890)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: RNA医薬 / miRNA / 核酸 / 生体機能利用 / 発現制御 / miRN / ゲノム

Research Abstract

The artificial microRNAs (miRNAs) that can specifically distinguish and suppress two highly homologous genes, Smad2 and Smad3, were designed to study the potential of artificial miRNAs as therapeutic drugs. Additionally, a plasmid-based system for tissue-specific regulation system of miRNA expression was constructed, and this system was confirmed that it functioned in mouse embryonic stem (ES) cells. The transgenic mice having the miRNA regulation system generated from these ES cells are under analyses.

Research Products

• [Journal Article] Characterization of NaV1. 6-mediated Na+ currents in smooth muscle cells isolated from mouse vas deferens. (2010)
  • Author(s): Zhu HL, Shibata A, Inai T, Nomura M, Shibata Y, Brock JA, Teramoto N
  • Journal Title: Journal of Cell Physiology 223巻 Pages: 234-243
  • Peer Reviewed
• [Journal Article] Characterization of Na V1.6-mediated Na+ currents in smooth muscle cells isolated from mouse vas deferens. (2010)
  • Author(s): Zhu, et al.
  • Journal Title: Journal of Cell Physiology 223 Pages: 234-243
  • Peer Reviewed
• [Journal Article] Cole WC. ATP-sensitive K+ channels in pig urethral smooth muscle cells are heteromultimers of Kir6. 1 and Kir6. 2. (2009)
  • Author(s): Teramoto N, Zhu HL, Shibata A, Aishima M, Walsh EJ, Nagao M
  • Journal Title: American Journal of Physiology - Renal Physioligy 296巻 Pages: 107-117
  • Peer Reviewed
• [Journal Article] ATP-sensitive K+ channels in pig urethral smooth muscle cells are heteromultimers of Kir6.1 and Kir6.2 (2009)
  • Author(s): Teramoto, et.al.
  • Journal Title: American Journal of Physiology-Renal Physiology 296 Pages: 107-117
  • Peer Reviewed
• [Presentation] Stoichiometric Analysis of Short Hairpin RNA in Cultured Cells. (2009)
  • Author(s): 柴田篤志、金子沙世、寺本憲功、服巻保幸
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-12-12
• [Presentation] 統合失調症関連遺伝子Grm3ノックアウトマウスの作出と発現解析 (2009)
  • Author(s): 二井偉暢、岩城明子、佐々木達哉、柴田篤志、福吉由起、佐方功明、野村政壽、服巻保幸
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-12-12
• [Presentation] Stoichiometric Analysis of Short Hairpin RNA in Cultured Cells (2009)
  • Author(s): 柴田篤志
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-12-12
• [Presentation] miRNAクラスターの介在配列の長さに依存したmiRNAの非対称的発現. (2009)
  • Author(s): 柴田篤志、金子沙世、寺本憲功
  • Organizer: 日本薬理学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-03-17
• [Presentation] miRNAクラスターの介在配列の長さに依存したmiRNAの非対称的発現 (2009)
  • Author(s): 柴田篤志
  • Organizer: 日本薬理学会
  • Place of Presentation: 神奈川県横浜市
  • Year and Date: 2009-03-17
• [Presentation] Stoichiometric Analysis of Short Hairpin RNA in Cultured Cells. (2009)
  • Author(s): Shibata A, Kaneko S, Teramoto N
  • Organizer: Joint Symposium of the 5th Annual Meeting of the Oligonucleotide Therapeutics Society and the 19th Antisense Symposium.
  • Place of Presentation: 福岡県福岡市
• [Presentation] Stoichiometric Analysis of Short Hairpin RNA in Cultured Cells (2009)
  • Author(s): Atsushi Shibata
  • Organizer: Joint Symposium of the 5^<th> Annual Meeting of the Oligonucleotide Therapeutics Society and the 19^<th> Antisense Symposium
  • Place of Presentation: 福岡県福岡市