Genome-wide screening of functional ribozymes by in vivo selection

• Project/Area Number: 20710167
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: HAGIHARA Masaki Osaka University, 産業科学研究所, 助教 (40403000)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: リボザイム / in vivoセレクション / ゲノム

Research Abstract

In vitro selection provides one of the most powerful strategies for obtaining functional RNA molecules. The selection and evolution technique has produced RNA aptamers that specifically bind small molecules or ribozymes that catalyze a variety of chemical reactions from randomized pools of RNA. In this project, we performed an in vitro selection aimed at isolating functional RNAs from the human genome RNA library.

Research Products

• [Journal Article] Secondary Structure-Inducible Ligand Fluorescence Coupled with PCR (2009)
  • Author(s): Fumie Takei, Masako Igarashi, Masaki Hagihara, Yoshimi Oka, Yoshihiro Soya, Kazuhiko Nakatani
  • Journal Title: Angewandte Chemie International Edition 4 Pages: 7822-7824
  • Peer Reviewed
• [Journal Article] Small Molecule Affecting the Replication of Trinucleotide Repeat d(GAA)n (2009)
  • Author(s): Hanping He, Masaki Hagihara, Kazuhiko Nakatani
  • Journal Title: Chemistry A European Journal 15 Pages: 10641-10648
  • Peer Reviewed
• [Journal Article] Unstable Tandem Repeats in Promoters Confer Transcriptional Evolvability (2009)
  • Author(s): Marcelo D. Vinces, Matthieu Legendre, Marina Caldara, Masaki Hagihara, Kevin J. Verstrepen
  • Journal Title: Science 324 Pages: 1213-1216
  • Peer Reviewed
• [Journal Article] RNA aptamers that reversibly bind photoresponsive azobenzene-containing peptides (2009)
  • Author(s): Hayashi, Gosuke, Hagihara Masaki, Nakatani Kazuhiko
  • Journal Title: Chemistry A European Journal 15 Pages: 424-432
  • Peer Reviewed
• [Journal Article] Unstable Tandem Repeats in Promoters Confer Transcriptional Evolvability (2009)
  • Author(s): Marcelo D.Vinces, Matthieu Legendre, Marina Caldara, Masaki Hagihara, Kevin J.Verstrepen
  • Journal Title: Science 324巻 Pages: 1213-1216
  • Peer Reviewed
• [Journal Article] Small Molecule Affecting the Replication of Trinucleotide Repeat d(GAA) n. (2009)
  • Author(s): Hanping He, Masaki Hagihara, Kazuhiko Nakatani
  • Journal Title: Chemistry-A European Journal 15巻 Pages: 10641-10648
  • Peer Reviewed
• [Journal Article] Secondary Structure-Inducible Ligand Fluorescence Coupled with PCR (2009)
  • Author(s): Fumie Takei, Masako Igarashi, Masaki Hagihara, Yoshimi Oka, Yoshihiro Soya, Kazuhiko Nakatani
  • Journal Title: Angewandte Chemie International Edition 48巻 Pages: 7822-7824
  • Peer Reviewed
• [Journal Article] RNA aptamers that reversibly bind photoresponsive azobenzene-containing peptides (2009)
  • Author(s): Hayashi, Gosuke, Hagihara Masaki, Nakatani Kazuhiko
  • Journal Title: Chemistry 15 Pages: 424-432
  • Peer Reviewed
• [Journal Article] Ligand Binding to Tandem G-quadruplexes from Human Telomeric DNA (2008)
  • Author(s): Li-Ping Bai, Masaki Hagihara, Zhi-Hong Jiang, Kazuhiko Nakatani
  • Journal Title: ChemBioChem 9 Pages: 2583-2587
  • Peer Reviewed
• [Journal Article] Genotyping by allele-specific l-DNA-tagged PCR (2008)
  • Author(s): Hayashi, Gosuke, Hagihara, Masaki; Nakatani, Kazuhiko
  • Journal Title: Journal of Biotechnology 135 Pages: 157-160
  • Peer Reviewed
• [Journal Article] Context-dependent fluorescence detection of a phosphorylated tyrosine residue by a ribonucleopeptide (2008)
  • Author(s): Hasegawa, Tetsuya; Hagihara, Masaki; Fukuda, Masatora; Nakano, Shun; Fujieda, Nobutaka; Morii, Takashi
  • Journal Title: Journal of the American Chemical Society 130 Pages: 8804-8812
  • Peer Reviewed
• [Journal Article] DNA Labeling by Ligand Inducible Secondary Structure (2008)
  • Author(s): Peng, Tao; He, Hanping; Hagihra, Masaki; Nakatani, Kazuhiko
  • Journal Title: ChemBioChem 9 Pages: 1893-1897
  • Peer Reviewed
• [Journal Article] DNA Labeling by Ligand Inducible Secondary Structure (2008)
  • Author(s): Peng, Tao ; He, Hanping ; Hagihra, Masaki ; Nakatani, Kazuhiko
  • Journal Title: Chem Bio Chem 9 Pages: 1893-1897
  • Peer Reviewed
• [Journal Article] Context-dependent fluorescence detection of a phosphorylated tyrosine residue by aribonucleopeptide (2008)
  • Author(s): Hasegawa, Tetsuya ; Hagihara, Masaki ; Fukuda, Masatora ; Nakano, Shun ; Fujieda, Nobutaka ; Morii, Takashi
  • Journal Title: Journal of the American Chemical Society 130 Pages: 8804-8812
  • Peer Reviewed
• [Journal Article] Genotyping by allele-specific 1-DNA-tagged PCR (2008)
  • Author(s): Hayashi, Gosuke, Hagihara, Masaki ; Nakatani, Kazuhiko
  • Journal Title: Journal of Biotechnology 135 Pages: 157-160
  • Peer Reviewed
• [Journal Article] Ligand Binding to Tandem G-quadruplexes from Human Telomeric DNA (2008)
  • Author(s): Li-Ping Bai, Masaki Hagihara, Zhi-Hong Jiang and Kazuhiko Nakatani
  • Journal Title: Chem Bio Chem 9 Pages: 2583-2587
  • Peer Reviewed
• [Presentation] RNA四重鎖構造を介した遺伝子発現制御 (2010)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学(大阪府)
  • Year and Date: 2010-03-27
• [Presentation] RNA四重鎖構造を介した遺伝子発現制御 (2010)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学 大阪
  • Year and Date: 2010-03-27
• [Presentation] mRNAの非翻訳領域に存在する四重鎖構造 (2009)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: 日本ケミカルバイオロジー研究会第4回年会
  • Place of Presentation: 神戸市産業振興センター(兵庫県)
  • Year and Date: 2009-05-19
• [Presentation] mRNAの非翻訳領域に存在する四重鎖構造 (2009)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: 日本ケミカルバイオロジー研究会 第4回年会
  • Place of Presentation: 神戸市産業振興センター
  • Year and Date: 2009-05-19
• [Presentation] (3)萩原正規、中谷和彦、グアニンリッチRNA配列に構造変化を誘起する小分子化合物 (2008)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: 第3回バイオ関連化学合同シンポジウム2008
  • Place of Presentation: 東京工業大学すずかけ台キャンパス(神奈川県)
• [Presentation] グアニンリッチRNA配列に構造変化を誘起する小分子化合物 (2008)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: 第3回バイオ関連化学合同シンポジウム2008
  • Place of Presentation: 東京工業大学すずかけ台キャンパス
• [Remarks]
  • URL: http://www.sanken.osaka-u.ac.jp/labs/rbc/member/hagihara.html