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Genome-wide screening of functional ribozymes by in vivo selection

Research Project

Project/Area Number 20710167
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Living organism molecular science
Research InstitutionOsaka University

Principal Investigator

HAGIHARA Masaki  Osaka University, 産業科学研究所, 助教 (40403000)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsリボザイム / in vivoセレクション / ゲノム
Research Abstract

In vitro selection provides one of the most powerful strategies for obtaining functional RNA molecules. The selection and evolution technique has produced RNA aptamers that specifically bind small molecules or ribozymes that catalyze a variety of chemical reactions from randomized pools of RNA. In this project, we performed an in vitro selection aimed at isolating functional RNAs from the human genome RNA library.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (23 results)

All 2010 2009 2008 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Secondary Structure-Inducible Ligand Fluorescence Coupled with PCR2009

    • Author(s)
      Fumie Takei, Masako Igarashi, Masaki Hagihara, Yoshimi Oka, Yoshihiro Soya, Kazuhiko Nakatani
    • Journal Title

      Angewandte Chemie International Edition 4

      Pages: 7822-7824

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Small Molecule Affecting the Replication of Trinucleotide Repeat d(GAA)n2009

    • Author(s)
      Hanping He, Masaki Hagihara, Kazuhiko Nakatani
    • Journal Title

      Chemistry A European Journal 15

      Pages: 10641-10648

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unstable Tandem Repeats in Promoters Confer Transcriptional Evolvability2009

    • Author(s)
      Marcelo D. Vinces, Matthieu Legendre, Marina Caldara, Masaki Hagihara, Kevin J. Verstrepen
    • Journal Title

      Science 324

      Pages: 1213-1216

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] RNA aptamers that reversibly bind photoresponsive azobenzene-containing peptides2009

    • Author(s)
      Hayashi, Gosuke, Hagihara Masaki, Nakatani Kazuhiko
    • Journal Title

      Chemistry A European Journal 15

      Pages: 424-432

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unstable Tandem Repeats in Promoters Confer Transcriptional Evolvability2009

    • Author(s)
      Marcelo D.Vinces, Matthieu Legendre, Marina Caldara, Masaki Hagihara, Kevin J.Verstrepen
    • Journal Title

      Science 324巻

      Pages: 1213-1216

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Small Molecule Affecting the Replication of Trinucleotide Repeat d(GAA) n.2009

    • Author(s)
      Hanping He, Masaki Hagihara, Kazuhiko Nakatani
    • Journal Title

      Chemistry-A European Journal 15巻

      Pages: 10641-10648

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Secondary Structure-Inducible Ligand Fluorescence Coupled with PCR2009

    • Author(s)
      Fumie Takei, Masako Igarashi, Masaki Hagihara, Yoshimi Oka, Yoshihiro Soya, Kazuhiko Nakatani
    • Journal Title

      Angewandte Chemie International Edition 48巻

      Pages: 7822-7824

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA aptamers that reversibly bind photoresponsive azobenzene-containing peptides2009

    • Author(s)
      Hayashi, Gosuke, Hagihara Masaki, Nakatani Kazuhiko
    • Journal Title

      Chemistry 15

      Pages: 424-432

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ligand Binding to Tandem G-quadruplexes from Human Telomeric DNA2008

    • Author(s)
      Li-Ping Bai, Masaki Hagihara, Zhi-Hong Jiang, Kazuhiko Nakatani
    • Journal Title

      ChemBioChem 9

      Pages: 2583-2587

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genotyping by allele-specific l-DNA-tagged PCR2008

    • Author(s)
      Hayashi, Gosuke, Hagihara, Masaki; Nakatani, Kazuhiko
    • Journal Title

      Journal of Biotechnology 135

      Pages: 157-160

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Context-dependent fluorescence detection of a phosphorylated tyrosine residue by a ribonucleopeptide2008

    • Author(s)
      Hasegawa, Tetsuya; Hagihara, Masaki; Fukuda, Masatora; Nakano, Shun; Fujieda, Nobutaka; Morii, Takashi
    • Journal Title

      Journal of the American Chemical Society 130

      Pages: 8804-8812

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA Labeling by Ligand Inducible Secondary Structure2008

    • Author(s)
      Peng, Tao; He, Hanping; Hagihra, Masaki; Nakatani, Kazuhiko
    • Journal Title

      ChemBioChem 9

      Pages: 1893-1897

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA Labeling by Ligand Inducible Secondary Structure2008

    • Author(s)
      Peng, Tao ; He, Hanping ; Hagihra, Masaki ; Nakatani, Kazuhiko
    • Journal Title

      Chem Bio Chem 9

      Pages: 1893-1897

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Context-dependent fluorescence detection of a phosphorylated tyrosine residue by aribonucleopeptide2008

    • Author(s)
      Hasegawa, Tetsuya ; Hagihara, Masaki ; Fukuda, Masatora ; Nakano, Shun ; Fujieda, Nobutaka ; Morii, Takashi
    • Journal Title

      Journal of the American Chemical Society 130

      Pages: 8804-8812

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotyping by allele-specific 1-DNA-tagged PCR2008

    • Author(s)
      Hayashi, Gosuke, Hagihara, Masaki ; Nakatani, Kazuhiko
    • Journal Title

      Journal of Biotechnology 135

      Pages: 157-160

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ligand Binding to Tandem G-quadruplexes from Human Telomeric DNA2008

    • Author(s)
      Li-Ping Bai, Masaki Hagihara, Zhi-Hong Jiang and Kazuhiko Nakatani
    • Journal Title

      Chem Bio Chem 9

      Pages: 2583-2587

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] RNA四重鎖構造を介した遺伝子発現制御2010

    • Author(s)
      萩原正規、中谷和彦
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学(大阪府)
    • Year and Date
      2010-03-27
    • Related Report
      2009 Final Research Report
  • [Presentation] RNA四重鎖構造を介した遺伝子発現制御2010

    • Author(s)
      萩原正規、中谷和彦
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学 大阪
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] mRNAの非翻訳領域に存在する四重鎖構造2009

    • Author(s)
      萩原正規、中谷和彦
    • Organizer
      日本ケミカルバイオロジー研究会第4回年会
    • Place of Presentation
      神戸市産業振興センター(兵庫県)
    • Year and Date
      2009-05-19
    • Related Report
      2009 Final Research Report
  • [Presentation] mRNAの非翻訳領域に存在する四重鎖構造2009

    • Author(s)
      萩原正規、中谷和彦
    • Organizer
      日本ケミカルバイオロジー研究会 第4回年会
    • Place of Presentation
      神戸市産業振興センター
    • Year and Date
      2009-05-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] (3)萩原正規、中谷和彦、グアニンリッチRNA配列に構造変化を誘起する小分子化合物2008

    • Author(s)
      萩原正規、中谷和彦
    • Organizer
      第3回バイオ関連化学合同シンポジウム2008
    • Place of Presentation
      東京工業大学すずかけ台キャンパス(神奈川県)
    • Related Report
      2009 Final Research Report
  • [Presentation] グアニンリッチRNA配列に構造変化を誘起する小分子化合物2008

    • Author(s)
      萩原正規、中谷和彦
    • Organizer
      第3回バイオ関連化学合同シンポジウム2008
    • Place of Presentation
      東京工業大学すずかけ台キャンパス
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.sanken.osaka-u.ac.jp/labs/rbc/member/hagihara.html

    • Related Report
      2009 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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