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Quantification and real time observation of calcium in living calcareous foraminifera

Research Project

Project/Area Number 20740301
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Stratigraphy/Paleontology
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

TOYOFUKU Takashi  Japan Agency for Marine-Earth Science and Technology, 海洋・極限環境生物圏領域, チームリーダー (30371719)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsバイオミネラリゼーション / 石灰質有孔虫 / 原生生物 / 海洋酸性化 / 炭酸カルシウム / 飼育実験 / 古生物学 / 地球生物学 / バイオミネラリゼーシヨン / カルシウムイメージング / 生物源炭酸塩 / 古海洋学 / 海洋生物学 / 実験古生物学
Research Abstract

本研究では炭酸カルシウムを主成分とする殻を持つ,海洋に生息する原生生物である石灰質有孔虫の石灰化過程とカルシウムの取り込みについて検討した.市販されているカルシウムプローブ(Fluo-3AM)を海産無脊椎動物に初めて応用し,細胞内のカルシウムイオン分布を観察した.その結果,細胞内に取り込まれた海水からカルシウムを取り込んでいることが示唆された.また,チャンバー形成時にはカルシウムイオンが細胞質と共に石灰化部位に輸送される様子が明らかになった.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (26 results)

All 2009 2008

All Journal Article (13 results) (of which Peer Reviewed: 6 results) Presentation (13 results)

  • [Journal Article] Foraminifera promote calcification by elevating their intracellular pH2009

    • Author(s)
      de Nooijer, L.J., Toyofuku, T., Kitazato, H.
    • Journal Title

      Proc. Nat. Ac. Sci. 106

      Pages: 15374-15378

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 地球化学・地球生物学分野への分子イメージング手法の応用2009

    • Author(s)
      豊福高志, 小栗一将, de Nooijer, L.J., 野牧秀隆, 土屋正史, 北里洋
    • Journal Title

      第四回学際領域における分子イメージングフォーラム (CD-ROM)

    • NAID

      120006828812

    • Related Report
      2009 Final Research Report
  • [Journal Article] Foraminifera promote calcification by elevating their intracellular pH2009

    • Author(s)
      de Nooijer, L.J.
    • Journal Title

      Proc. Nat. Ac. Sci. 106

      Pages: 15374-15378

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 地球化学・地球生物学分野への分子イメージング手法の応用2009

    • Author(s)
      豊福高志
    • Journal Title

      JAXA SP(Special Publication) (CD-ROM)

    • NAID

      120006828812

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 地球化学・地球生物学分野への分子イメージング手法の応用2009

    • Author(s)
      豊福高志
    • Journal Title

      学際領域における分子イメージングフォーラム、JAXA SP(Special Publication) (受理)

    • NAID

      120006828812

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Intracellular pH distribution in foraminifera determined by the fluorescent probe HPTS2008

    • Author(s)
      de Nooijer, L.J., Toyofuku, T., Oguri, K., Nomaki, H., Kitazato, H.
    • Journal Title

      Limnol. Oceanogr. -Methods 6

      Pages: 610-618

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Real-time visualization of calcium ion activity in shallow benthic foraminiferal cells using the fluorescent indicator Fluo-3 AM2008

    • Author(s)
      Toyofuku, T., de Nooijer, L.J., Yamamoto, H., Kitazato, H.
    • Journal Title

      Geochem. Geophys. Geosyst. 9

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 有孔虫細胞内の環境可視化手法の開発と応用-バイオミネラリゼーションの解明を目指して-2008

    • Author(s)
      豊福高志, de Nooijer, L.J., 野牧秀隆, 小栗一将, 土屋正史, 北里洋
    • Journal Title

      月刊地球 30(7)

      Pages: 346-351

    • Related Report
      2009 Final Research Report
  • [Journal Article] Intracellular pH distribution in foraminifera determined by the fluorescent probe HPTS2008

    • Author(s)
      De Nooijer, L. J
    • Journal Title

      Limnology and Oceanography: Methods 6

      Pages: 610-618

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 深海低層生態系変動のリアルタイム観測2008

    • Author(s)
      北里 洋
    • Journal Title

      月刊地球 30

      Pages: 396-402

    • Related Report
      2008 Annual Research Report
  • [Journal Article] 有孔虫細胞内の環境可視化手法の開発と応用-バイオミネラリゼーションの解明を目指して-2008

    • Author(s)
      豊福 高志
    • Journal Title

      月刊地球 30

      Pages: 346-351

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Real-time visualization of calcium ion activity in shallow benthic foraminiferal cells using the fluorescent indicator Fluo-3 AM2008

    • Author(s)
      Toyofuku, T.
    • Journal Title

      Geochemistry Geophysics Geosystems 9

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assessing influence of diagenetic carbonate dissolution on planktonic foraminiferal Mg/Ca in the southeastern Arabian Sea over the past 450 ka:Comparison between Globigerinoides ruber and Globigerinoides sacculifer2008

    • Author(s)
      Tachikawa, K.
    • Journal Title

      Geochemistry Geophysics Geosystems 9

    • Related Report
      2008 Annual Research Report
  • [Presentation] Internal environmental control within Foraminifera through calcification2009

    • Author(s)
      豊福高志, 小栗一将, de Nooijer, L.J., 野牧秀隆, 土屋正史, 北里洋
    • Organizer
      地球惑星科学合同大会
    • Place of Presentation
      千葉
    • Year and Date
      2009-05-19
    • Related Report
      2009 Final Research Report
  • [Presentation] Intracellular control on calcification in benthic foraminifera2009

    • Author(s)
      de Nooijer, L.J., Toyofuku, T., Langer, G., Nehrke, G., Kitazato, H., Bijma, J.
    • Organizer
      European Geosciences Union General Assembly 2009
    • Place of Presentation
      Vienna
    • Year and Date
      2009-04-21
    • Related Report
      2009 Final Research Report
  • [Presentation] Fluorescent observations of calcium ion activity in living benthic foraminifera2009

    • Author(s)
      Toyofuku, T. de Nooijer, L.J., Kitazato, H.
    • Organizer
      European Geosciences Union General Assembly 2009
    • Place of Presentation
      Vienna
    • Year and Date
      2009-04-21
    • Related Report
      2009 Final Research Report
  • [Presentation] Fluorescent observations of calcium ion activity in living benthic foraminifera2009

    • Author(s)
      Toyofuku, T
    • Organizer
      European Geosciences Union General Assembly 2009 (EGU2009)
    • Place of Presentation
      Austria Center Vienna,ウイーン
    • Year and Date
      2009-04-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] 環境自律制御システムを用いた有孔虫飼育実験2009

    • Author(s)
      豊福高志
    • Organizer
      MRC2009研究集会
    • Place of Presentation
      新宿区、国立科学博物館新宿分館
    • Year and Date
      2009-03-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] 底生有孔虫の石灰化過程とMg含有量2009

    • Author(s)
      豊福高志, de Nooijer, L.J., 北里洋
    • Organizer
      日本古生物学会第158回例会, 琉球大学
    • Place of Presentation
      琉球大学
    • Year and Date
      2009-01-31
    • Related Report
      2009 Final Research Report
  • [Presentation] 地球化学・地球生物学分野への分子イメージング手法の応用2008

    • Author(s)
      豊福高志, 小栗一将, de Nooijer, L.J., 野牧秀隆, 土屋正史, 北里洋
    • Organizer
      第四回学際領域における分子イメージングフォーラム
    • Place of Presentation
      早稲田大学
    • Year and Date
      2008-10-09
    • Related Report
      2009 Final Research Report
  • [Presentation] Real-time visualization of chemical environment in the living marine protist "foraminifera" by fluo-3 and HPTS2008

    • Author(s)
      Toyofuku, T., Nomaki., H., Oguri, K., Kitazto, H., de Nooijer, L. J
    • Organizer
      Japanese-German Joint Seminar -New Prospects of Molecular Imaging Technology for Interdisciplinary Research
    • Place of Presentation
      東北大学
    • Year and Date
      2008-09-03
    • Related Report
      2009 Final Research Report
  • [Presentation] カルシウム・pH蛍光指示薬を用いた観察から示唆される有孔虫の石灰化モデル2008

    • Author(s)
      豊福高志, de Nooijer, L.J., 野牧秀隆, 小栗一将, 北里洋
    • Organizer
      日本古生物学会2008年年会
    • Place of Presentation
      東北大学
    • Year and Date
      2008-07-06
    • Related Report
      2009 Final Research Report
  • [Presentation] カルシウム・pH蛍光指示薬を用いた観察から示唆される有孔虫の石灰化モデル2008

    • Author(s)
      豊福高志
    • Organizer
      日本古生物学会2008年年会
    • Place of Presentation
      仙台市、東北大学
    • Year and Date
      2008-07-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] Laboratory experimental evaluation of foraminiferal Mg/Ca2008

    • Author(s)
      Toyofuku, T.
    • Organizer
      地球惑星科学合同大会
    • Place of Presentation
      千葉市、幕張メッセ
    • Year and Date
      2008-05-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Foraminiferl calcification and the role of intracellular pH2008

    • Author(s)
      De Nooijer, L. J
    • Organizer
      the Micropaleontological Society's Foraminifera and Nanofossil Groups Joint Spring Meeting 2008
    • Place of Presentation
      チュービンゲン大学、チュービンゲン、ドイツ
    • Year and Date
      2008-05-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] A new model of foraminifera calcification pathways in shallow benthic foraminifera: miliolids vs hyaline species2008

    • Author(s)
      Toyofuku, T
    • Organizer
      the Micropaleontological Society's Foramini fera and Nanofossil Groups Joint Spring Meeting 2008
    • Place of Presentation
      チュービンゲン大学、チュービンゲン、ドイツ
    • Year and Date
      2008-05-15
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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