Electrochemical sensing for bacterial toxins using magnetic microbeads

• Project/Area Number: 20750057
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: University of Toyama
• Principal Investigator: KURAMITZ Hideki University of Toyama, 大学院・理工学研究部(理学), 准教授 (70397165)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 毒素タンパク質 / 電気化学検出 / 磁性マイクロ粒子 / 電極活性物質 / 対流ボルタンメトリー / 磁気分離

Research Abstract

The electrochemical analytical method for protein without addition of any reagents were developed. The protein as an analyte can be highly concentrated by magnetic recovery which contributes high sensitively for the assay. The changes in electrochemical response of the magnetic micro-beads modified an electroactive compound and protein recognition molecule were observed by binding the target protein. The application for cholera toxin assay has also been achieved.

Research Products

• [Journal Article] (2009)
  • Author(s): K. Sugawara, A. Yugami, N. Terui, H. Kuramitz
  • Journal Title: Analytical Sciences 25 Pages: 1365-1368
• [Journal Article] (2009)
  • Author(s): K. Sugawara, A. Yugami, H. Kuramitz
  • Journal Title: Analytical and Bioanalytical Chemistry 395 Pages: 767-772
• [Journal Article] (2009)
  • Author(s): 倉光英樹, 佐澤和人, 七山泰昭, 工藤千春, 川上貴教, 波多宣子, 田口茂
  • Journal Title: 水環境学会誌 Vol.32,No.6 Pages: 309-314
• [Journal Article] (2009)
  • Author(s): H. Kuramitz
  • Journal Title: Analytical and Bioanalytical Chemistry 394 Pages: 61-69
• [Journal Article] (2009)
  • Author(s): K. Sugawara, M. Kawai, G. Hirabayashi, H. Kuramitz
  • Journal Title: Analytical Sciences 25 Pages: 105-108
• [Journal Article] Magnetic microbead-based electrochemical immunoassays (2009)
  • Author(s): H.Kuramitz
  • Journal Title: Analytical and Bioanalytical Chemistry 394 Pages: 61-69
  • Peer Reviewed
• [Journal Article] Sensitivity of a glassy carbon electrode covered with a chitin film improved by the addition of carbon powder (2009)
  • Author(s): K.Sugawara, M.Kawai, G.Hirabayashi, H.Kuramitz
  • Journal Title: Analytical Sciences 25 Pages: 105-108
  • Peer Reviewed
• [Journal Article] マイクロスケール藻類生長阻害試験を用いた銅の毒性に対する溶存有機物質に含まれる抑制成分の探索 (2009)
  • Author(s): 倉光英樹, 佐澤和人, 七山泰昭, 工藤千春, 川上貴教, 波多宣子, 田口茂
  • Journal Title: 水環境学会誌 32 Pages: 309-314
  • NAID: 10024904615
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): H. Kuramitz, A. Piruska, H.B. Halsall, C.J. Seliskar, W.R. Heineman.
  • Journal Title: Analytical Chemistry 80 Pages: 9642-9648
• [Journal Article] (2008)
  • Author(s): K. Murai, M. Okano, H. Kuramitz, N. Hata, T. Kawakami, S. Taguchi.
  • Journal Title: Analytical Sciences 24 Pages: 1455-1459
• [Journal Article] (2008)
  • Author(s): K. Murai, M. Okano, H. Kuramitz, N. Hata, T. Kawakami, S. Taguchi.
  • Journal Title: Water Science and Technology 58 Pages: 1055-1060
• [Journal Article] (2008)
  • Author(s): M. Nakayama, N. Hata, H. Kuramitz, S. Taguchi.
  • Journal Title: Journal of Environmental Chemistry 18(3) Pages: 353-360
• [Journal Article] (2008)
  • Author(s): N. Hata, S. Hieda, M. Yamada, R. Yasui, H. Kuramitz, S. Taguchi.
  • Journal Title: Analytical Sciences 24 Pages: 925-928
• [Journal Article] (2008)
  • Author(s): K. Sugawara, A. Senbongi, N. Kamiya, G. Hirabayashi, H. Kuramitz.
  • Journal Title: Analytical Sciences 24 Pages: 717-720
• [Journal Article] (2008)
  • Author(s): K. Sugawara, A. Terauchi, N. Kamiya, G. Hirabayashi, H. Kuramitz.
  • Journal Title: Analytical Sciences 24 Pages: 583-587
• [Journal Article] (2008)
  • Author(s): 関山沙織, 倉光英樹, 波多宣子, 田口茂.
  • Journal Title: 分析化学 57 Pages: 613-618
• [Journal Article] Simultaneously multiselective spectroelectrochemical sensing for the interaction between protein and its ligand using redoxdye-Nile Blue as a label (2008)
  • Author(s): H. Kuramitz, A. Piruska, H. B. Halsall, C. J. Seliskar, and, W. R. Heineman
  • Journal Title: Analytical Chemistry 80 Pages: 9642-9648
  • Peer Reviewed
• [Journal Article] Membrane solubilization technique for spectrophotometric determin ation of trace formaldehyde in rainwater (2008)
  • Author(s): K. Murai, M. Okano, H. Kur amitz, N. Hata, T. Kawakami, and, S. Taguchi
  • Journal Title: Analytical Sciences 24 Pages: 1455-1459
  • Peer Reviewed
• [Journal Article] Voltammetric evaluation of the binding between wheat germ agglutinin and thionine/glucose-modified magnetic microbead (2008)
  • Author(s): K. Sugawara, A. Senbongi, N. Kamiya, G Hirabayashi, and, H. Kuramitz
  • Journal Title: Analytical Sciences 24 Pages: 717-720
  • Peer Reviewed
• [Presentation] 液滴試料の対流ボルタンメトリーによるイムノアッセイとバイオアッセイの高度化 (2009)
  • Author(s): 倉光英樹
  • Organizer: 第40回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 岐阜大学
  • Year and Date: 2009-11-07
• [Presentation] (2009)
  • Author(s): H Kuramitz, Y. Nanayama, K. Sazawa, N. Hata, S. Taguchi
  • Organizer: Asia Young Analytical Chemist Session (AYACS) 2009
  • Place of Presentation: KL, Malaysia, AYACS13.
  • Year and Date: 2009-08-10
• [Presentation] (2009)
  • Author(s): H. Kuramitz, M. Takeuchi, K. Sazawa, M. Ikeuchi, N. Hata, S. Taguchi
  • Organizer: 10th Asian Conference on Analytical Sciences 2009 (ASIANALYSIA X)
  • Place of Presentation: KL, Malaysia, BEF32
• [Presentation] (2009)
  • Author(s): 福島正巳・石田洋輔・重松聡子・倉光英樹
  • Organizer: 第25回日本腐植物質学会講演会
  • Place of Presentation: 姫路県立大学(姫路市)
• [Presentation] (2009)
  • Author(s): 原夕貴・倉光英樹・波多宣子・田口茂
  • Organizer: 第25回日本腐植物質学会講演会
  • Place of Presentation: 姫路県立大学(姫路市)
• [Presentation] (2009)
  • Author(s): 王文〓・倉光英樹・波多宣子・田口茂
  • Organizer: 第40回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 岐阜大学(岐阜市)
• [Presentation] (2009)
  • Author(s): 倉光英樹
  • Organizer: 第40回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 岐阜大学(岐阜市)
• [Presentation] (2009)
  • Author(s): 波多宣子・安井理恵・橋本真之介・倉光英樹・田口茂
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] (2009)
  • Author(s): 中川和也・波多宣子・倉光英樹・田口茂・千葉元
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] (2009)
  • Author(s): 由上麻子・高橋哲史・照井教文・倉光英樹・菅原一晴
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] (2009)
  • Author(s): 佐澤和人・倉光英樹・波多宣子・田口茂
  • Organizer: 日本分析化学会第58年会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] (2009)
  • Author(s): 橋本真之介・安井理恵・波多宣子・倉光英樹・田口茂
  • Organizer: 第28回分析化学中部夏期セミナー
  • Place of Presentation: サンレイク美浜(静岡県浜松市)
• [Presentation] (2009)
  • Author(s): 佐澤和人・七山泰昭・倉光英樹・波多宣子・田口茂
  • Organizer: 第28回分析化学中部夏期セミナー
  • Place of Presentation: サンレイク美浜(静岡県浜松市)
• [Presentation] (2009)
  • Author(s): 波多宣子・安井理恵・寺島俊平・倉光英樹・田口茂
  • Organizer: 第70回分析化学討論会
  • Place of Presentation: 和歌山
• [Presentation] (2009)
  • Author(s): 菅原一晴・由上麻子・照井教文・倉光英樹
  • Organizer: 第70回分析化学討論会
  • Place of Presentation: 和歌山
• [Presentation] (2009)
  • Author(s): 佐澤和人・倉光英樹・菅原一晴・波多宣子・田口茂
  • Organizer: 第70回分析化学討論会
  • Place of Presentation: 和歌山
• [Presentation] (2009)
  • Author(s): 村居景太・倉光英樹・波多宣子・田口茂
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 東京
• [Presentation] 抗体修飾型電気化学イオンチャンネルセンサーの開発 (2008)
  • Author(s): 倉光英樹・上野英志・波多宣子・田口茂
  • Organizer: 第69回分析化学討論会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-05-15
• [Presentation] (2008)
  • Author(s): 工藤千春・倉光英樹・波多宣子・田口茂
  • Organizer: 平成20年度日本水環境学会中部支部学術集会
  • Place of Presentation: 富山
• [Presentation] (2008)
  • Author(s): 佐澤和人・倉光英樹・波多宣子・田口茂
  • Organizer: 平成20年度日本水環境学会中部支部学術集会
  • Place of Presentation: 富山
• [Presentation] (2008)
  • Author(s): 中川和也・波多宣子・倉光英樹・田口茂・千葉元
  • Organizer: 平成20年度日本水環境学会中部支部学術集会
  • Place of Presentation: 富山
• [Presentation] (2008)
  • Author(s): 村居景太・新美紗恵子・倉光英樹・波多宣子・田口茂・川崎清人・木戸瑞佳
  • Organizer: 平成20年度日本水環境学会中部支部学術集会
  • Place of Presentation: 富山
• [Book] 自然と経済から見つめる北東アジアの環境(第11章水環境の汚染) (2009)
  • Author(s): 倉光英樹, 和田直也, 今村弘子(編著)
  • Publisher: 富山大学出版会
• [Book] 自然と経済から見つめる北東アジアの環境(第14章環境修復) (2009)
  • Author(s): 倉光英樹, 和田直也, 今村弘子(編著)
  • Publisher: 富山大学出版会
• [Book] 自然と経済から見つめる北東アジアの環境 (2009)
  • Author(s): 倉光英樹(共著)
  • Total Pages: 311
  • Publisher: 富山大学出版会