Development of a new method for chemical synthesis of aminoacylated tRNA.
| Project/Area Number |
20750129
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
OHKUBO Akihiro Tokyo Institute of Technology, 大学院・生命理工学研究科, 助教 (60376960)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | アミノシルtRNA / 核酸合成 / 保護基 / tRNA / 核酸 / 有機化学 / 高分子化学 |
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| Research Abstract |
We developed a new method for the thermolytic deprotection of amidine-type protecting groups on nucleosides and nucleotides under acidic conditions using HOBt and IMT. Moreover, our new strategy allowed the first synthesis of an RNA oligomer carrying a base-labile acyl group on adenine. These results have prompted us to study the introduction of various artificial aminoacyl groups into RNA oligomers. These additional studies are now underway.
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Report
(3 results)
Research Products
(35 results)
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[Journal Article] "Protected DNA Probes (PDP)" capable of strong hybridization without removal of base protecting groups2008
Author(s)
A. Ohkubo, R. Kasuya, K. Sakamoto, K. Miyata, H. Taguchi, H. Nagasawa, T. Tsukahara, T. Watanobe, Yoshiyuki Maki, Kohji Seio, Mitsuo Sekine
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Journal Title
Nucleic Acids Res. 36
Pages: 1952-1964
Related Report
Peer Reviewed
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[Journal Article] Protected DNA Probes' capable of strong hybridization without removal of base protecting groups2008
Author(s)
Ohkubo A, Kasuya R, Sakamoto K, Miyata K, Taguchi H, Nagasawa H, Tsukahara T, Watanobe T, Maki Y, Seio K, Sekine M.
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Journal Title
Nucleic Acids Research 36
Pages: 1952-1964
Related Report
Peer Reviewed
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