| Project/Area Number |
20750133
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
MIZUNO Toshihisa Nagoya Institute of Technology, 大学院・工学研究科, 助教 (90345950)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ナノバイオ / 蛋白質 / バイオテクノロジー |
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| Research Abstract |
As a model system of protein-protein interaction, we chose the T7 RNA polymerase-T7 lysozyme pair in this research. By inserting the originally designed coiled-coil protein, which transform its tertiary structure from random coil to coiled-coil structure by the specific ligand peptide, into the T7 lysozyme, we succeeded in tuning the interaction between T7 RNA polymerase and T7 lysozyme by the binding of specific ligand peptide. On the other hand, we also succeeded in constructing the ligand-binding protein, which exhibits a reversible structural alteration following the specific ligand binding. Interestingly, this protein can work even in the in vivo circumstances. By combining this designed ligand-binding protein with various target natural proteins, ligand-binding tuning of protein-protein interactions should be possible in future.
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