Chemical probe for amplification of genetic signal

• Project/Area Number: 20750146
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ABE Hiroshi The Institute of Physical and Chemical Research, 伊藤ナノ医工学研究室, 専任研究員 (80415067)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 増幅 / 遺伝子 / RNA / 蛍光 / 検出 / 蛍光発生化合物 / 遺伝子検出 / 核酸連結反応

Research Abstract

Our aim is to develop a new method for genetic detection which can amplify signal through multiple chemical reactions. The method will be applied for gene diagnosis in living cells.

Research Products

• [Journal Article] DNA templated nucleophilic aromatic substitution reactions for fluorogenic sensing of oligonucleotides (2009)
  • Author(s): Aya Shibata, Hiroshi Abe, Mika Ito, Yuko Kondo, Shigeru Shimizu, Kyoko Aikawa Yoshihiro Ito
  • Journal Title: Chemical Communication 43 Pages: 6586-6588
  • Peer Reviewed
• [Journal Article] Reduction-triggered Fluorescent Amplification Probe for Detection of endogenous RNAs in Living Human Cells (2009)
  • Author(s): Kazuhiro Furukawa, Hiroshi Abe, Kayoko Hibino, Yasushi Sako, Satoshi Tsuneda, Yoshihiro Ito
  • Journal Title: Bioconjugate Chemistry 20 Pages: 1026-1036
  • Peer Reviewed
• [Journal Article] Reduction-triggered red fluorescent probes for dual-color detection of oligonucleotide sequences (2009)
  • Author(s): Kazuhiro Furukawa, Hiroshi Abe, Jin Wang, Miwako Uda, Satoshi Tsuneda, Yoshihiro Ito
  • Journal Title: Organic Biomolecular Chemistry 7 Pages: 671-677
  • Peer Reviewed
• [Journal Article] DNA templated nucleophilic aromatic substitution reactions for fluorogenic sensing of oligonucleotides (2009)
  • Author(s): Aya Shibata et al.
  • Journal Title: Chemical Communication 43 Pages: 6586-6588
  • Peer Reviewed
• [Journal Article] Characterisation of a New Fluorogenic Substrate for Microsomal Glutathione Transferase 1 (2009)
  • Author(s): Johan Alander et al.
  • Journal Title: Analytical Biochemistry 390 Pages: 52-56
  • Peer Reviewed
• [Journal Article] Reduction-triggered Fluorescent Amplification Probe for Detection of end ogenous RNAs in Living Human Cells (2009)
  • Author(s): Kazuhiro Furukawa, et al.
  • Journal Title: Bioconjugate Chemistry 20 Pages: 1026-1036
  • Peer Reviewed
• [Journal Article] Rhodamine-based fluorogenic probe for imaging biological thiol (2008)
  • Author(s): Aya Shibata, et al.
  • Journal Title: Bio. Org. Med. Chem. Lett. 18 Pages: 2246-2249
  • Peer Reviewed
• [Presentation] 芳香族求核置換反応を引き金とした新規蛍光発生分子システムを導入した遺伝子検出プローブの開発 (2010)
  • Author(s): 伊藤美香, 柴田綾, 阿部洋, 中嶋裕子, 清水繁, 相川京子, 伊藤嘉浩
  • Organizer: 日本薬学会第130回年会
  • Place of Presentation: 岡山
• [Presentation] Chemical reaction triggered fluorogenic probe for RNA detection in living cells (2009)
  • Author(s): Hiroshi Abe
  • Organizer: BIT's 3^<rd> world congress of Gene-2009
  • Place of Presentation: Foshan, China
  • Year and Date: 2009-12-05
• [Presentation] RNA情報を釣り針とする微生物生細胞の分離・濃縮技術の開発 (2009)
  • Author(s): 古川 和寛, 阿部洋, 常田聡, 伊藤嘉浩
  • Organizer: 第61回日本生物工学会大会
  • Place of Presentation: 名古屋
• [Presentation] 細胞内RNA検出プローブの開発 (2008)
  • Author(s): 阿部洋 、古川和寛、王瑾、烏田美和子、常田聡、伊藤嘉浩
  • Organizer: 第18回アンチセンスシンポジウム
  • Place of Presentation: 岐阜
• [Presentation] 新規蛍光化合物を導入したプローブよる遺伝子シグナルの化学増幅 (2008)
  • Author(s): 阿部洋 、古川和寛、王瑾 、烏田美和子、常田聡、伊藤嘉浩
  • Organizer: 第34回反応と合成の進歩シンポジウム
  • Place of Presentation: 大阪、ポスター
• [Presentation] 新規蛍光化合物を導入した化学反応プローブによる遺伝子シグナルの増幅 (2008)
  • Author(s): 阿部洋
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 横浜
• [Book] 細胞機能制御高分子と再生医療高分子 (2009)
  • Author(s): 伊藤嘉浩、北嶋隆、阿部洋
• [Remarks]
  • URL: http://www.riken.go.jp/r-world/research/lab/wako/medical/index.html
• [Patent(Industrial Property Rights)] 蛍光発生分子 (2008)
  • Inventor(s): 阿部洋、伊藤嘉浩、古川和寛、王瑾
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Filing Date: 2008-01-09